公开(公告)号：US20240174987A1

公开(公告)日：2024-05-30

申请号：US18525234

申请日：2023-11-30

Applicant: AMYRIS, INC.

Inventor： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC: C12N9/10 ,  A23L2/60 ,  A23L27/30 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017 ,  A23V2002/00

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20130316383A1

公开(公告)日：2013-11-28

申请号：US13870911

申请日：2013-04-25

Applicant: AMYRIS, INC.

Inventor： Sunil BAJAD ,  Michael LEAVELL

IPC: G01N27/62

CPC classification number: G01N27/62 ,  C12P5/007 ,  C12P7/04 ,  C12P23/00

Abstract: The present invention provides methods and compositions for monitoring cofactors and metabolites of a metabolic pathway of interest. The subject compositions and methods are particularly suited for monitoring the mevalonate pathway in a variety of cells. The invention also provides fermentation methods for the production of isoprenoids.

Abstract translation: 本发明提供了用于监测感兴趣的代谢途径的辅因子和代谢物的方法和组合物。 主题组合物和方法特别适用于监测各种细胞中的甲羟戊酸途径。 本发明还提供了用于生产类异戊二烯的发酵方法。

公开(公告)号：US20210355458A1

公开(公告)日：2021-11-18

申请号：US17382963

申请日：2021-07-22

Applicant: AMYRIS, INC.

Inventor： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US20190185826A1

公开(公告)日：2019-06-20

申请号：US16323756

申请日：2017-08-11

Applicant: AMYRIS, INC.

Inventor： Lishan ZHAO ,  Wenzong LI ,  Gale WICHMANN ,  Aditi KHANKHOJE ,  GARCIA DE GONZALO, Chantal GARCIA DE GONZALO ,  Tina MAHATDEJKUL-MEADOWS ,  Shaina JACKSON ,  Michael LEAVELL ,  Darren PLATT

IPC: C12N9/10 ,  C12N15/52 ,  C12P19/56 ,  C12P19/18 ,  A23L2/60 ,  A23L27/30

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.