公开(公告)号：US12221641B2

公开(公告)日：2025-02-11

申请号：US17285888

申请日：2019-10-14

Applicant: AMYRIS, INC.

Inventor： Gale A. Wichmann ,  Wenzong Li ,  Sean Lund ,  Shaina J. Jackson ,  Chantal V. Garcia De Gonzalo ,  Hailley Warbington ,  Yi Xiong ,  Svetlana Alekseyevna Borisova

IPC: C12P19/56 ,  C12N1/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12R1/865

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Stevia rebaudiana kaurenoic acid hydroxylase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US12065685B1

公开(公告)日：2024-08-20

申请号：US18368369

申请日：2023-09-14

Applicant: AMYRIS, INC.

Inventor： Sean Lund ,  Gale Wichmann

IPC: C12P19/56 ,  C12N15/81

CPC classification number: C12P19/56 ,  C12N15/81 ,  C12Y106/02004 ,  C12Y114/14 ,  C12Y205/01029 ,  C12Y402/03019 ,  C12Y505/01012

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of UGT76G1. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of rebaudioside M.