公开(公告)号：US11866738B2

公开(公告)日：2024-01-09

申请号：US17382963

申请日：2021-07-22

Applicant: AMYRIS, INC.

Inventor： Lishan Zhao ,  Wenzong Li ,  Gale Wichmann ,  Aditi Khankhoje ,  Chantal Garcia De Gonzalo ,  Tina Mahatdejkul-Meadows ,  Shaina Jackson ,  Michael Leavell ,  Darren Platt

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017 ,  A23V2002/00

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US12065685B1

公开(公告)日：2024-08-20

申请号：US18368369

申请日：2023-09-14

Applicant: AMYRIS, INC.

Inventor： Sean Lund ,  Gale Wichmann

IPC: C12P19/56 ,  C12N15/81

CPC classification number: C12P19/56 ,  C12N15/81 ,  C12Y106/02004 ,  C12Y114/14 ,  C12Y205/01029 ,  C12Y402/03019 ,  C12Y505/01012

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of UGT76G1. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of rebaudioside M.

公开(公告)号：US12264351B2

公开(公告)日：2025-04-01

申请号：US16637188

申请日：2018-08-10

Applicant: AMYRIS, INC.

Inventor： Gale Wichmann ,  Aditi Khankhoje ,  Tina Mahatdejkul-Meadows

IPC: C12N9/20 ,  C12N15/81 ,  C12P7/40 ,  C12P19/56 ,  C12N1/18 ,  C12R1/865

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Pisum sativum kaurene oxidase or its variant kaurene oxidase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US11091743B2

公开(公告)日：2021-08-17

申请号：US16323756

申请日：2017-08-11

Applicant: AMYRIS, INC.

Inventor： Lishan Zhao ,  Wenzong Li ,  Gale Wichmann ,  Aditi Khankhoje ,  Chantal Garcia De Gonzalo ,  Tina Mahatdejkul-Meadows ,  Shaina Jackson ,  Michael Leavell ,  Darren Platt

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.