公开(公告)号：US12221641B2

公开(公告)日：2025-02-11

申请号：US17285888

申请日：2019-10-14

Applicant: AMYRIS, INC.

Inventor： Gale A. Wichmann ,  Wenzong Li ,  Sean Lund ,  Shaina J. Jackson ,  Chantal V. Garcia De Gonzalo ,  Hailley Warbington ,  Yi Xiong ,  Svetlana Alekseyevna Borisova

IPC: C12P19/56 ,  C12N1/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12R1/865

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Stevia rebaudiana kaurenoic acid hydroxylase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US11866738B2

公开(公告)日：2024-01-09

申请号：US17382963

申请日：2021-07-22

Applicant: AMYRIS, INC.

Inventor： Lishan Zhao ,  Wenzong Li ,  Gale Wichmann ,  Aditi Khankhoje ,  Chantal Garcia De Gonzalo ,  Tina Mahatdejkul-Meadows ,  Shaina Jackson ,  Michael Leavell ,  Darren Platt

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

CPC classification number: C12N9/1051 ,  A23L2/60 ,  A23L27/36 ,  C12N15/52 ,  C12N15/81 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01017 ,  A23V2002/00

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

公开(公告)号：US11091743B2

公开(公告)日：2021-08-17

申请号：US16323756

申请日：2017-08-11

Applicant: AMYRIS, INC.

Inventor： Lishan Zhao ,  Wenzong Li ,  Gale Wichmann ,  Aditi Khankhoje ,  Chantal Garcia De Gonzalo ,  Tina Mahatdejkul-Meadows ,  Shaina Jackson ,  Michael Leavell ,  Darren Platt

IPC: C12N9/10 ,  A23L2/60 ,  C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12N15/81 ,  C12P19/18

Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.