公开(公告)号：US20230366040A1

公开(公告)日：2023-11-16

申请号：US18021719

申请日：2021-08-26

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander GREEN ,  Kaiyue WU

IPC: C12Q1/70 ,  C12Q1/6886 ,  C12Q1/6897

CPC classification number: C12Q1/701 ,  C12Q1/6886 ,  C12Q1/6897 ,  C12Q2600/156 ,  C12Q1/6804

Abstract: Provided herein are methods and compositions for rapid, highly sensitive detection of a target nucleic acid in a sample, which may be indicative of the presence of a pathogen, disease condition, or disease predisposition. In particular, provided herein is a low-cost, portable method for detecting target nucleic acid sequences that rapidly provides reliable, visible test results and does not require elaborate biosafety precautions or sophisticated laboratory equipment.

公开(公告)号：US20230323487A1

公开(公告)日：2023-10-12

申请号：US18043012

申请日：2021-08-26

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander GREEN ,  Zhaoqing YAN ,  Anli TANG

IPC: C12Q1/70 ,  C12Q1/6825 ,  C12N15/115 ,  C12Q1/6818

CPC classification number: C12Q1/701 ,  C12Q1/6825 ,  C12N2310/16 ,  C12Q1/6818 ,  C12N15/115

Abstract: Provided herein are methods and compositions for rapid, highly sensitive detection of SARS-CoV-2, the causative agent of the COVID-19 pandemic or other target nucleic acids. The methods are low-cost and can be implemented in a portable format that does not require elaborate biosafety precautions or sophisticated laboratory equipment.

公开(公告)号：US20230212698A1

公开(公告)日：2023-07-06

申请号：US18001395

申请日：2021-06-11

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

Inventor： Alexander GREEN ,  Keith PARDEE ,  Margot KARLIKOW ,  Kaiyue WU ,  Masoud NOROUZI

IPC: C12Q1/6897 ,  C12Q1/70

CPC classification number: C12Q1/6897 ,  C12Q1/701

Abstract: Provided herein are methods and compositions for rapid, highly sensitive detection of SARS-CoV-2 in biological samples. In particular, provided herein is a rapid, low-cost method for detecting SARS-CoV-2 that provides reliable, visible test results and does not require PCR reagents, elaborate biosafety precautions, or sophisticated laboratory equipment.

公开(公告)号：US20190256898A1

公开(公告)日：2019-08-22

申请号：US16342722

申请日：2017-10-17

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander GREEN ,  Duo MA ,  Anli TANG

IPC: C12Q1/6825

Abstract: Methods and compositions for detecting the presence of a pathogen are described. In particular, this document provides a method of detecting a pathogen-associated nucleic acid in a biological sample of a subject, where the method comprises using one or more unimolecular aptamer-based sensors comprising an aptamer-fluorophore complex and an amplification step to detect the pathogen-associated nucleic acid. Methods specific for detecting the presence of malaria and other mosquito-borne virus infections are also provided.

公开(公告)号：US20230089497A1

公开(公告)日：2023-03-23

申请号：US17909345

申请日：2021-03-03

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Qi ZHANG ,  Xiao WANG ,  Alexander GREEN ,  Duo MA ,  Fuqing WU ,  Kylie STANDAGE-BEIER ,  Xingwen CHEN

IPC: C12N15/67

Abstract: The present invention provides a new class of RNA modules, referred to as degradation tuning RNAs (dtRNAs), which form stabilizing secondary structures. Also provided are methods of using dtRNAs to modulate the stability of RNAs. DNA constructs including a promoter that is operably connected to a sequence encoding the dtRNA are also provided.

公开(公告)号：US20190185856A1

公开(公告)日：2019-06-20

申请号：US16322799

申请日：2017-08-01

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander GREEN ,  Yu Zhou

IPC: C12N15/113 ,  H03K19/20

CPC classification number: C12N15/113 ,  C12N15/67 ,  C12N2310/122 ,  C12N2310/531 ,  H03K19/20

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.