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1.发明申请MUTANT GAMMA-GLUTAMYLTRANSFERASE, AND A METHOD FOR PRODUCING GAMMA-GLUTAMYLVALYLGLYCINE OR A SALT THEREOF 有权突变GAMMA-GLUTAMYLTRANSFERASE，以及一种生产GAMMA-谷氨酰乙酰胆碱或其盐的方法公开(公告)号：US20140212920A1
公开(公告)日：2014-07-31
申请号：US14243458
申请日：2014-04-02
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki NOZAKI , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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Patent Agency Ranking
2.发明授权公开(公告)号：US08753841B2
公开(公告)日：2014-06-17
申请号：US13713915
申请日：2012-12-13
Applicant: Ajinomoto Co., Inc.
Inventor： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
Abstract translation: 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。
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公开(公告)号：US09580696B2
公开(公告)日：2017-02-28
申请号：US14243458
申请日：2014-04-02
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki Nozaki , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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公开(公告)号：US09677106B2
公开(公告)日：2017-06-13
申请号：US15235311
申请日：2016-08-12
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki Nozaki , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
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公开(公告)号：US20160355861A1
公开(公告)日：2016-12-08
申请号：US15235311
申请日：2016-08-12
Applicant: Ajinomoto Co., Inc.
Inventor： Hiroyuki NOZAKI , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC classification number: C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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6.发明申请ENZYME THAT CATALYZES A PEPTIDE-FORMING REACTION FROM A CARBOXY COMPONENT AND AN AMINE COMPONENT, MICROBE PRODUCING THE SAME, AND A METHOD OF PRODUCING A DIPEPTIDE USING THE ENZYME OR MICROBE 有权催化由羧基组分和胺组分形成的肽形成反应的酶，以及生产它们的微生物以及使用酶或MICROBE生产肽的方法公开(公告)号：US20130122544A1
公开(公告)日：2013-05-16
申请号：US13713915
申请日：2012-12-13
Applicant: Ajinomoto Co., Inc.
Inventor： Seiichi HARA , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
Abstract translation: 公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。 制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞 与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。
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