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公开(公告)号：US20160355861A1
公开(公告)日：2016-12-08
申请号：US15235311
申请日：2016-08-12
申请人： Ajinomoto Co., Inc.
发明人： Hiroyuki NOZAKI , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC分类号： C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
摘要： A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
摘要翻译： 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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公开(公告)号：US20180195103A1
公开(公告)日：2018-07-12
申请号：US15911731
申请日：2018-03-05
申请人： AJINOMOTO CO., INC.
发明人： Ayako SASAHARA , Takayuki ITO , Hiroyuki NOZAKI
CPC分类号： C12P21/02 , C07K5/0806 , C07K5/0819 , C12N9/93 , C12N15/09 , C12R1/19 , C12Y603/02002 , C12Y603/02003
摘要： A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDK) is reduced as an expression host, γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.
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3.发明申请MUTANT GAMMA-GLUTAMYLTRANSFERASE, AND A METHOD FOR PRODUCING GAMMA-GLUTAMYLVALYLGLYCINE OR A SALT THEREOF 有权突变GAMMA-GLUTAMYLTRANSFERASE，以及一种生产GAMMA-谷氨酰乙酰胆碱或其盐的方法公开(公告)号：US20140212920A1
公开(公告)日：2014-07-31
申请号：US14243458
申请日：2014-04-02
申请人： Ajinomoto Co., Inc.
发明人： Hiroyuki NOZAKI , Isao Abe , Jun Takakura , Rie Takeshita , Hideyuki Suzuki , Shunichi Suzuki
CPC分类号： C12P21/02 , C07K5/0215 , C07K5/0819 , C12N9/104 , C12N15/70 , C12Y203/02002 , Y02P20/52
摘要： A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
摘要翻译： 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰基供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。
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公开(公告)号：US20230399673A1
公开(公告)日：2023-12-14
申请号：US18454052
申请日：2023-08-22
申请人： AJINOMOTO CO., INC.
发明人： Ayako SASAHARA , Takayuki ITO , Hiroyuki NOZAKI
CPC分类号： C12P21/02 , C12N1/205 , C07K5/0806 , C07K5/0819 , C12N9/93 , C12N15/09
摘要： A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDK) is reduced as an expression host, γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.
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