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1.发明申请NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYSIS APPARATUS, AND NUCLEIC ACID ANALYSIS METHOD 审中-公开核酸分析装置，核酸分析装置和核酸分析方法公开(公告)号：US20120064527A1
公开(公告)日：2012-03-15
申请号：US13322203
申请日：2010-05-24
CPC分类号： G01N21/6486 , C12Q1/6837 , G01N21/6452 , Y10T436/143333 , C12Q2565/513
摘要： The present invention relates to a nucleic acid analysis device in a nucleic acid analysis apparatus, whereby waste of reaction spots on the nucleic acid analysis device is eliminated and leakage of fluorescence excitation light to unobserved nucleic acid measurement regions is minimized. Specifically, the nucleic acid analysis device has a plurality of nucleic acid measurement regions, which are characterized in that one nucleic acid measurement region is disposed at a sufficient distance from the other nucleic acid measurement regions such that the other nucleic acid measurement regions do not enter an irradiation region.
摘要翻译： 本发明涉及核酸分析装置中的核酸分析装置，其中消除了核酸分析装置上的反应点的浪费，使荧光激发光向未观察到的核酸测量区域的泄漏最小化。 具体而言，核酸分析装置具有多个核酸测定区域，其特征在于，将一个核酸测定区域配置在距离其他核酸测定区域足够的距离，使得其他核酸测定区域不进入 照射区域。
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公开(公告)号：US08389959B2
公开(公告)日：2013-03-05
申请号：US13147147
申请日：2009-11-30
申请人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
发明人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01J3/10 , G01J3/14 , G01J3/36 , G01J3/4406 , G01N21/6452
摘要： The present invention has an object to provide a method for efficiently detecting an image with a smaller number of pixels.The invention relates to fluorescence analysis which uses a substrate having a plurality of regions for being capable of immobilizing biologically-related molecules in positions of lattice points of a lattice structure, and which causes the fluorescence from a certain lattice point to be wavelength-dispersed in a direction other than the direction toward the adjacent closest lattice point. According to an embodiment, for example, the number of pixels of a two-dimensional sensor required for fluorescence analysis of the regions with the biologically-related molecules immobilized can be set to several hundred times to fifty times smaller than that in the conventional case without degrading the measurement accuracy. This can achieve the improvement of throughput, reduction in price, and/or improvement of the operability of an analyzing device.
摘要翻译： 本发明的目的是提供一种用于有效检测具有较少像素数的图像的方法。 本发明涉及使用具有多个区域的基板的荧光分析，其能够将生物相关分子固定在格子结构的格点的位置，并且使得来自特定格子点的荧光波长分散在 除了朝向相邻最近格子点的方向之外的方向。 根据一个实施例，例如，具有生物相关分子固定的区域的荧光分析所需的二维传感器的像素数量可以设定为比常规情况下少几百次到五十次，而没有 降低测量精度。 这可以实现分析装置的吞吐量的提高，价格的降低和/或改善可操作性。
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公开(公告)号：US08004673B2
公开(公告)日：2011-08-23
申请号：US12292444
申请日：2008-11-19
IPC分类号： G01J3/30
CPC分类号： G01N21/648 , G01N21/6456 , G01N2021/6423
摘要： A metallic structure is provided on a surface of a substrate. A component having a longer wavelength than excitation light is detected from luminescence from fixation positions of biomolecules and emitted from a material other than the biomolecules, and is used for photometrical analysis. As the structure, usable is a particulate (a metallic structure of a size not larger than a wavelength of the excitation light), a minute protrusion, or a thin film with minute apertures, which are made of a metal such as gold, chrome, silver or aluminum. In the case of the particulate or the minute protrusion, photoluminescence of the structure is detected with a biomolecule being fixed thereon. In the case of the thin film with minute apertures, Raman scattered light of specimen solution around the biomolecules, and photoluminescence of the metallic structure near the biomolecules are detected with biomolecules being fixed in the apertures.
摘要翻译： 金属结构设置在基板的表面上。 从生物分子的固定位置的发光和从生物分子以外的材料发射的发光中检测到具有比激发光更长波长的成分，并用于光度分析。 作为结构，可以使用由金，铬等金属制成的颗粒（不大于激发光的波长的金属结构），微小突起或微小孔的薄膜， 银或铝。 在颗粒或微小突起的情况下，通过固定有生物分子来检测结构的光致发光。 在具有微小孔径的薄膜的情况下，拉曼散射生物分子周围的样品溶液的光，并且用生物分子固定在孔中来检测生物分子附近的金属结构的光致发光。
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公开(公告)号：US08680483B2
公开(公告)日：2014-03-25
申请号：US13141127
申请日：2009-12-22
IPC分类号： G01J1/58
CPC分类号： G01N21/6452 , G01N2021/6421 , G01N2021/6441 , G01N2021/6478
摘要： A fluorescence detector in which a sample substrate is provided with a structure unit comprising a prism or a diffraction grating. After excitation light falling on the sample substrate is totally reflected at a biomolecule-immobilized face that is located in the opposite side of the structure unit, the structure unit allows the emission of the reflected light therefrom. To ensure multiple visual field measurement, a sample substrate-driving unit is provided to scan the sample substrate.
摘要翻译： 一种荧光检测器，其中样品基板设置有包括棱镜或衍射光栅的结构单元。 落在样品基板上的激发光在位于结构单元的相对侧的生物分子固定面全面反射，结构单元允许从其发射反射光。 为了确保多个视野测量，提供了样品基板驱动单元以扫描样品基板。
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公开(公告)号：US20090128807A1
公开(公告)日：2009-05-21
申请号：US12292444
申请日：2008-11-19
IPC分类号： G01N21/31
CPC分类号： G01N21/648 , G01N21/6456 , G01N2021/6423
摘要： A metallic structure is provided on a surface of a substrate. A component having a longer wavelength than excitation light is detected from luminescence from fixation positions of biomolecules and emitted from a material other than the biomolecules, and is used for photometrical analysis. As the structure, usable is a particulate (a metallic structure of a size not larger than a wavelength of the excitation light), a minute protrusion, or a thin film with minute apertures, which are made of a metal such as gold, chrome, silver or aluminum. In the case of the particulate or the minute protrusion, photoluminescence of the structure is detected with a biomolecule being fixed thereon. In the case of the thin film with minute apertures, Raman scattered light of specimen solution around the biomolecules, and photoluminescence of the metallic structure near the biomolecules are detected with biomolecules being fixed in the apertures.
摘要翻译： 金属结构设置在基板的表面上。 从生物分子的固定位置的发光和从生物分子以外的材料发射的发光中检测到具有比激发光更长波长的成分，并用于光度分析。 作为结构，可以使用由金，铬等金属制成的颗粒（不大于激发光的波长的金属结构），微小突起或微小孔的薄膜， 银或铝。 在颗粒或微小突起的情况下，通过固定有生物分子来检测结构的光致发光。 在具有微小孔的薄膜的情况下，拉曼散射生物分子周围的样品溶液的光，并且用生物分子固定在孔中来检测生物分子附近的金属结构的光致发光。
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公开(公告)号：US20110272596A1
公开(公告)日：2011-11-10
申请号：US13141127
申请日：2009-12-22
IPC分类号： G01N21/64
CPC分类号： G01N21/6452 , G01N2021/6421 , G01N2021/6441 , G01N2021/6478
摘要： A fluorescence detector in which a sample substrate is provided with a structure unit comprising a prism or a diffraction grating. After excitation light falling on the sample substrate is totally reflected at a biomolecule-immobilized face that is located in the opposite side of the structure unit, the structure unit allows the emission of the reflected light therefrom. To ensure multiple visual field measurement, a sample substrate-driving unit is provided to scan the sample substrate.
摘要翻译： 一种荧光检测器，其中样品基板设置有包括棱镜或衍射光栅的结构单元。 落在样品基板上的激发光在位于结构单元的相对侧的生物分子固定面全面反射，结构单元允许从其发射反射光。 为了确保多个视野测量，提供了样品基板驱动单元以扫描样品基板。
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公开(公告)号：US20110284768A1
公开(公告)日：2011-11-24
申请号：US13147147
申请日：2009-11-30
申请人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
发明人： Satoshi Takahashi , Tsuyoshi Sonehara , Tomoyuki Sakai , Takanobu Haga , Hirokazu Kato , Nobutaka Kumazaki , Takuya Matsui
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01J3/10 , G01J3/14 , G01J3/36 , G01J3/4406 , G01N21/6452
摘要： The present invention has an object to provide a method for efficiently detecting an image with a smaller number of pixels.The invention relates to fluorescence analysis which uses a substrate having a plurality of regions for being capable of immobilizing biologically-related molecules in positions of lattice points of a lattice structure, and which causes the fluorescence from a certain lattice point to be wavelength-dispersed in a direction other than the direction toward the adjacent closest lattice point. According to an embodiment, for example, the number of pixels of a two-dimensional sensor required for fluorescence analysis of the regions with the biologically-related molecules immobilized can be set to several hundred times to fifty times smaller than that in the conventional case without degrading the measurement accuracy. This can achieve the improvement of throughput, reduction in price, and/or improvement of the operability of an analyzing device.
摘要翻译： 本发明的目的是提供一种用于有效检测具有较少像素数的图像的方法。 本发明涉及使用具有多个区域的基板的荧光分析，其能够将生物相关分子固定在格子结构的格点的位置，并且使得来自特定格子点的荧光波长分散在 除了朝向相邻最近格子点的方向之外的方向。 根据一个实施例，例如，具有生物相关分子固定的区域的荧光分析所需的二维传感器的像素数量可以设定为比常规情况下少几百次到五十次，而没有 降低测量精度。 这可以实现分析装置的吞吐量的提高，价格的降低和/或改善可操作性。
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公开(公告)号：US20120097864A1
公开(公告)日：2012-04-26
申请号：US13380251
申请日：2010-06-04
IPC分类号： G01N21/64
CPC分类号： G01N21/6452 , G01N21/6428 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441
摘要： Provided are a method and an apparatus for easily identifying and detecting fluorescent material types captured in respective reaction regions of a substrate, particularly, a method and an apparatus for identifying and measuring the fluorescence intensities of a plurality of fluorescent materials using a small pixel count. The fluorescence intensities of four or more types of fluorescent materials are divided by a dividing section at ratios different at least for each fluorescence maximum wavelength range, and are detected by at least one detector including pixels for detecting the light fluxes divided at the different ratios. The type of the fluorescent material is determined on the basis of the ratio of the detected fluorescence intensity of the same detection portion, and the fluorescence intensity is measured.
摘要翻译： 提供一种用于容易地识别和检测在基板的各个反应区域中捕获的荧光材料类型的方法和装置，特别是用于使用小像素数来识别和测量多种荧光材料的荧光强度的方法和装置。 四种或更多类型的荧光材料的荧光强度由至少对于每个荧光最大波长范围不同的比率除以分割部分，并且由至少一个检测器检测，包括用于检测以不同比率分割的光通量的像素。 基于检测出的相同检测部分的荧光强度的比例来测定荧光体的种类，测定荧光强度。
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公开(公告)号：US08680484B2
公开(公告)日：2014-03-25
申请号：US13380251
申请日：2010-06-04
IPC分类号： G01N21/64
CPC分类号： G01N21/6452 , G01N21/6428 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441
摘要： Provided are a method and an apparatus for easily identifying and detecting fluorescent material types captured in respective reaction regions of a substrate, particularly, a method and an apparatus for identifying and measuring the fluorescence intensities of a plurality of fluorescent materials using a small pixel count. The fluorescence intensities of four or more types of fluorescent materials are divided by a dividing section at ratios different at least for each fluorescence maximum wavelength range, and are detected by at least one detector including pixels for detecting the light fluxes divided at the different ratios. The type of the fluorescent material is determined on the basis of the ratio of the detected fluorescence intensity of the same detection portion, and the fluorescence intensity is measured.
摘要翻译： 提供一种用于容易地识别和检测在基板的各个反应区域中捕获的荧光材料类型的方法和装置，特别是用于使用小像素数来识别和测量多种荧光材料的荧光强度的方法和装置。 四种或更多类型的荧光材料的荧光强度由至少对于每个荧光最大波长范围不同的比率除以分割部分，并且由至少一个检测器检测，包括用于检测以不同比率分割的光通量的像素。 基于检测出的相同检测部分的荧光强度的比例来测定荧光体的种类，测定荧光强度。
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公开(公告)号：US20090168061A1
公开(公告)日：2009-07-02
申请号：US12339285
申请日：2008-12-19
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/6428
摘要： A fluorescent detection apparatus relates to an analysis technique for qualitatively detecting or quantifying biomolecules by producing an evanescent field on a surface of a substrate, exciting fluorescently labelled biomolecules on the substrate surface in the evanescent field, and detecting the resultant fluorescent light emitted from the biomolecules. The fluorescent detection apparatus has a configuration in which a well is provided in a surface opposing to a sample substrate of a prism, the well is filled with a matching liquid, and the matching liquid is filled between the sample substrate and the prism, thereby improving operability and providing a stable evanescent field.
摘要翻译： 荧光检测装置涉及用于通过在基底表面上产生ev逝场来激发生物分子进行定性检测或定量的分析技术，在ev逝场中激发荧光标记的生物分子，并检测从生物分子发射的荧光 。 荧光检测装置具有在与棱镜的样品基板相对的表面上设置有阱的构造，在该阱中填充有匹配液体，并且将匹配液体填充在样品基板与棱镜之间，由此提高 可操作性，并提供稳定的消逝场。
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