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公开(公告)号：US20080014634A1
公开(公告)日：2008-01-17
申请号：US11637395
申请日：2006-12-11
摘要： The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
摘要翻译： 本发明提供了选择性克隆同源双链核酸分子的方法，特别是通过使用含有条件表达和/或条件活性失配识别酶的宿主细胞株，例如编码基因的基因的温度敏感变体 来自噬菌体T4的核酸内切酶VII。 使用该宿主菌株，本发明的特征在于选择缺少PCR产生突变的PCR产物的新型克隆方法。
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公开(公告)号：US20050186676A1
公开(公告)日：2005-08-25
申请号：US11088322
申请日：2005-03-23
摘要： The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
摘要翻译： 本发明提供了选择性克隆同源双链核酸分子的方法，特别是通过使用含有条件表达和/或条件活性失配识别酶的宿主细胞株，例如编码基因的基因的温度敏感变体 来自噬菌体T4的核酸内切酶VII。 使用该宿主菌株，本发明的特征在于选择缺少PCR产生突变的PCR产物的新型克隆方法。
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公开(公告)号：US20070072297A1
公开(公告)日：2007-03-29
申请号：US11523422
申请日：2006-09-18
申请人： Henry Ji , Alan Greener , Joseph Sorge , John Bauer , Richard Gibbs , Carsten-Peter Carstens
发明人： Henry Ji , Alan Greener , Joseph Sorge , John Bauer , Richard Gibbs , Carsten-Peter Carstens
摘要： The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.
摘要翻译： 本发明提供共价连接核酸分子的方法和分子克隆方法。 所述方法提供通过拓扑异构酶和DNA连接酶连接或同时连接侧翼或载体核酸分子与核酸插入物分子。 该方法提供核酸分子的定向和非定向共价连接和克隆。
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公开(公告)号：US07109178B2
公开(公告)日：2006-09-19
申请号：US10057050
申请日：2002-01-25
申请人： Henry Ji , Alan Greener , Joseph A. Sorge , John Bauer , Richard Gibbs , Carsten-Peter Carstens
发明人： Henry Ji , Alan Greener , Joseph A. Sorge , John Bauer , Richard Gibbs , Carsten-Peter Carstens
IPC分类号： A61K31/711
摘要： The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.
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公开(公告)号：US20070122827A1
公开(公告)日：2007-05-31
申请号：US11546675
申请日：2006-10-11
CPC分类号： C12Q1/6816 , C12Q1/6844 , C12Q2537/155 , C12Q2521/101 , C12Q2525/307 , C12Q2521/501 , C12Q2525/155
摘要： Provided herein are methods, compositions and kits for detecting a target nucleic acid. A target nucleic acid can be produced, for example, by a cleavage reaction in an assay for detection of biological samples. In one aspect, a described method comprises the following steps: hybridizing the target nucleic acid having a 5′ end and a 3′ end to a probe to form a circular hybridization complex; forming a covalent circular target nucleic acid using the probe as a template for nucleic acid synthesis; and detecting the covalent circular target nucleic acid.
摘要翻译： 本文提供了用于检测靶核酸的方法，组合物和试剂盒。 靶核酸可以例如通过用于检测生物样品的测定中的裂解反应产生。 一方面，所述方法包括以下步骤：将具有5'末端和3'末端的靶核酸与探针杂交以形成环状杂交复合物; 使用探针作为核酸合成的模板形成共价环状靶核酸; 并检测共价环状靶核酸。
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公开(公告)号：US20060166332A1
公开(公告)日：2006-07-27
申请号：US11284343
申请日：2005-11-21
摘要： The invention provides cells and methods of circularizing linear DNA molecules. The cell is an isolated Escherichia coli cell which transiently expresses the Cre recombinase protein from an integrated Cre recombinase gene, and which is at least transiently repressed for RecBCD activity. The cells are used in a method of circularizing a linear DNA molecule comprising at least two loxP sites. The DNA molecule is introduced into the cells, and the linear DNA molecule is joined at said loxp sites.
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公开(公告)号：US20080050743A1
公开(公告)日：2008-02-28
申请号：US11821286
申请日：2007-06-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2565/201 , C12Q2521/301 , C12Q2521/337
摘要： The present invention provides methods, kits and compositions for the detection of an analyte. The invention is particularly suited for the detection and quantification of analytes in solution. In the methods of the invention a complex is formed between two or more analyte specific probes (ASP) and an analyte. The reactive moieties of the probes interact upon the binding of the analyte specific probes to the analyte. The reactive moieties generate a nucleic acid cleavage product which is detected and indicative of the presence of the analyte.
摘要翻译： 本发明提供了用于检测分析物的方法，试剂盒和组合物。 本发明特别适用于溶液中分析物的检测和定量。 在本发明的方法中，在两种或多种分析物特异性探针（ASP）和分析物之间形成复合物。 探针的反应性部分在分析物特异性探针与分析物结合时相互作用。 反应性部分产生核酸切割产物，其被检测并指示分析物的存在。
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8.发明申请Compositions and methods for the detection of a nucleic acid using circular probes in a cleavage reaction 审中-公开在切割反应中使用环状探针检测核酸的组合物和方法公开(公告)号：US20070248965A1
公开(公告)日：2007-10-25
申请号：US11603569
申请日：2006-11-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2537/157 , C12Q2525/307 , C12Q2521/301
摘要： The invention provides a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample by forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with a probe, nucleic acid polymerase, nuclease and optionally a primer. The target nucleic acid is extended and circularized and a cleavage structure is formed. The cleavage structure is cleaved with a nuclease to generate a nucleic acid fragment which is indicative of the presence of a target nucleic acid sequence in the sample.
摘要翻译： 本发明提供了一种生成指示样品中靶核酸序列存在的信号的方法，其通过形成切割结构，通过将包含靶核酸序列的样品与探针，核酸聚合酶，核酸酶和任选的引物 。 靶核酸被延伸和环化并形成裂解结构。 切割结构用核酸酶切割以产生指示样品中靶核酸序列存在的核酸片段。
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公开(公告)号：US20080213760A1
公开(公告)日：2008-09-04
申请号：US11773353
申请日：2007-07-03
申请人： Jeffrey C. Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
发明人： Jeffrey C. Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
IPC分类号： C12Q1/68
CPC分类号： C07H21/04
摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.
摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了由这些多核苷酸编码的嵌合蛋白质。 本发明还提供了使用本发明的多核苷酸检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。
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公开(公告)号：US20090075259A1
公开(公告)日：2009-03-19
申请号：US11897886
申请日：2007-08-31
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12N15/62 , G01N33/581 , G01N2333/9015 , G01N2458/10 , C12Q2563/125 , C12Q2537/125 , C12Q2521/107 , C12Q2521/501 , C12Q2521/519
摘要： The present invention provides methods, kits, and compositions for the detection of an analyte. The invention is particularly suited for the detection and quantification of an analyte in a sample. In the methods of the invention a complex is formed between an analyte specific binding agent and an analyte. The analyte specific agents are coupled to an enzyme possessing an activity that produces a PCR template indicative of the presence of the analyte. Amplification and detection of the PCR template yields a sensitive and quantitative measurement of analyte concentration.
摘要翻译： 本发明提供了用于检测分析物的方法，试剂盒和组合物。 本发明特别适用于检测和定量样品中的分析物。 在本发明的方法中，在分析物特异性结合剂和分析物之间形成复合物。 分析物特异性试剂与具有产生指示分析物存在的PCR模板的活性的酶偶联。 扩增和检测PCR模板产生敏感和定量的分析物浓度测量。
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