公开(公告)号：US20190300846A1

公开(公告)日：2019-10-03

申请号：US16339164

申请日：2017-10-03

Applicant: Alma Mater Studiorum-Università di Bologna

Inventor： Stefania RAPINO ,  Francesco ZERBETTO ,  Gastone CASTELLANI ,  Stefano SALVIOLI ,  Beatrice FRABONI ,  Isabella ZIRONI ,  Maria CONTE ,  Claudio FRANCESCHI ,  Daniela SALVATORE ,  Maila BECCONI

IPC: C12N5/00 ,  C12N9/04 ,  C12N11/04 ,  C12N11/12 ,  C12N5/09 ,  C12N9/08 ,  C12N11/08 ,  G01N33/50

Abstract: The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.

公开(公告)号：US20240254436A1

公开(公告)日：2024-08-01

申请号：US18426809

申请日：2024-01-30

Applicant: Alma Mater Studiorum - Università di Bologna

Inventor： Stefania RAPINO ,  Francesco ZERBETTO ,  Gastone CASTELLANI ,  Stefano SALVIOLI ,  Beatrice FRABONI ,  Isabella ZIRONI ,  Maria CONTE ,  Claudio FRANCESCHI ,  Daniela SALVATORE ,  Maila BECCONI

IPC: C12N5/00 ,  C12N5/09 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  G01N33/50

CPC classification number: C12N5/0018 ,  C12N5/0068 ,  C12N5/0693 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0036 ,  C12N9/0053 ,  C12N9/0061 ,  C12N9/0065 ,  C12N9/0069 ,  C12N9/0093 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  C12Y101/03004 ,  C12Y106/03001 ,  C12Y109/03001 ,  C12Y110/03002 ,  C12Y111/01006 ,  C12Y113/12004 ,  C12Y117/03002 ,  G01N33/5008 ,  C12N2500/02 ,  C12N2501/71 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/74 ,  C12N2533/76 ,  C12N2533/78 ,  C12N2533/80 ,  C12N2537/10

Abstract: The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.