公开(公告)号：US20190091699A1

公开(公告)日：2019-03-28

申请号：US16113793

申请日：2018-08-27

Applicant: ANCERA, INC.

Inventor： Hur KOSER

IPC: B03C1/28 ,  B03C1/033 ,  B01L3/00

Abstract: Systems, methods and devices are presented for extracting target particles within a ferrofluid medium. In some embodiments, a fluidic channel receives a flow of a mix of one or more types of target particles, where at least one magnetic field source is configured to react with the flow such that a force (indirect or direct) is placed on the particles of the mix, across the width and/or the height of the fluidic channel. An extraction opening placed on one wall is provided and configured to extract at least one type of target particle.

公开(公告)号：US20190120822A1

公开(公告)日：2019-04-25

申请号：US15982926

申请日：2018-05-17

Applicant: ANCERA, INC.

Inventor： Hur KOSER

IPC: G01N33/50 ,  B01L3/00 ,  C12M1/02 ,  C12M3/00 ,  C12M3/06 ,  C12Q1/02 ,  C12N13/00 ,  B01L7/04 ,  G01N1/40 ,  B03C1/32 ,  B03C1/28

Abstract: A system for determining drug effectiveness on a plurality of cells is described. The system includes flowing a ferrofluid mixed with a plurality of biological cells through an inlet portion of a cartridge, the cartridge comprising a plurality of microfluidic channels, the inlet is in communication with a portion of each of the plurality of channels, applying a magnetic field proximate at least one of the inlet portion and the plurality of micro-channels, wherein the magnetic field is configured to apply an indirect force on the mix, separating biologic cells according to at least a first type as the mix flows in a first direction; and directing at least the first type of cells toward a first sensor functionalized with receptors via at least one of the micro-channels, the sensor arranged proximate to a second portion of at least one of the micro-channels downstream from the first inlet portion.

公开(公告)号：US20190118190A1

公开(公告)日：2019-04-25

申请号：US16013793

申请日：2018-06-20

Applicant: Ancera, Inc.

Inventor： Hur KOSER

IPC: B03C1/32 ,  C12N13/00 ,  C02F1/44 ,  C02F1/48 ,  B03C1/253 ,  B03C1/033 ,  B03C1/023 ,  B03C1/28

Abstract: A device and method for extracting particles contained in a ferrofluid medium are provided. Such methods may comprise suspending particles of different sizes in a ferrofluid medium and containing the ferrofluid medium in a cylindrical reservoir, and applying a first magnetic field to at least a portion of the reservoir. The first magnetic field is configured to indirectly exert a force on at least a portion of the particles of a predetermined size, and direct the portion of particles in a desired direction.

公开(公告)号：US20160299132A1

公开(公告)日：2016-10-13

申请号：US14777512

申请日：2014-03-17

Applicant: ANCERA, INC.

Inventor： Hur KOSER

IPC: G01N33/543 ,  B03C1/32 ,  B03C1/01 ,  G01N15/14 ,  B01L3/00

CPC classification number: G01N33/54366 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/288 ,  B03C1/32 ,  B03C2201/18 ,  B03C2201/20 ,  B03C2201/26 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N27/44786 ,  G01N33/54313 ,  G01N35/0098 ,  G01N2015/1415 ,  G01N2015/1486 ,  G01N2015/149

Abstract: Some embodiments of the present disclosure are directed to systems and methods for separating, directing, and/or extracting a target molecule from a mix of molecules and may comprise a plurality of non-magnetic beads suspended in a ferro fluid, where the non-magnetic beads may be functionalized with at least one predetermined first molecule configured to bind with a target particle. A microfluidic device may be included which may comprise at least one microfluidic channel, the device configured to dynamically and/or statically receive an amount of the mix. Magnetic field means may be included and may be configured to apply a magnetic field to at least a portion of the at least one channel to exert an indirect force on the non-magnetic beads in the ferro fluid mix, and separate the non-magnetic beads from the ferrofluid. The beads may then be directed to at least one receptor region. At least one outlet may be provided which is arranged to be in communication with the at least one microfluidic channel, the at least one outlet may be configured to receive and extract the separated non-magnetic beads from the ferrofluid.

Abstract translation: 本公开的一些实施方案涉及用于从分子混合物分离，引导和/或提取靶分子的系统和方法，并且可以包括悬浮在铁流体中的多个非磁珠，其中非磁性 珠子可以被至少一个预定的第一分子功能化，其被配置为与目标颗粒结合。 可以包括微流体装置，其可以包括至少一个微流体通道，该装置被配置为动态地和/或静态地接收一定量的混合物。 可以包括磁场装置并且可以被配置为将磁场施加到至少一个通道的至少一部分，以在铁液流体混合物中的非磁珠上施加间接力，并将非磁珠 从铁磁流体。 然后将珠子引导至至少一个受体区域。 可以提供至少一个出口，其布置成与至少一个微流体通道连通，所述至少一个出口可以被配置为从铁磁流体接收和提取分离的非磁珠。

公开(公告)号：US20160299126A1

公开(公告)日：2016-10-13

申请号：US14777507

申请日：2014-03-17

Applicant: ANCERA, INC.

Inventor： Hur KOSER

IPC: G01N33/50 ,  B01L7/04 ,  C12M1/02 ,  C12M3/00 ,  C12M3/06 ,  B01L3/00 ,  G01N1/40

CPC classification number: G01N33/5008 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/04 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0864 ,  B01L2300/0877 ,  B01L2300/1883 ,  B01L2400/043 ,  B01L2400/0487 ,  B03C1/288 ,  B03C1/32 ,  B03C2201/26 ,  C12M23/16 ,  C12M23/42 ,  C12M41/18 ,  C12N13/00 ,  C12Q1/02 ,  G01N1/40 ,  G01N2001/4038

Abstract: A system for determining drug effectiveness on a plurality of cells is described. The system includes flowing a ferro-fluid mixed with a plurality of biological cells through an inlet portion of a cartridge, the cartridge comprising a plurality of micro-fluidic channels, the inlet is in communication with a portion of each of the plurality of channels, applying a magnetic field proximate at least one of the inlet portion and the plurality of micro-channels, wherein the magnetic field is configured to apply an indirect force on the mix, separating biologic cells according to at least a first type as the mix flows in a first direction; and directing at least the first type of cells toward a first sensor functionalized with receptors via at least one of the micro-channels, the sensor arranged proximate to a second portion of at least one of the micro-channels downstream from the first inlet portion.

Abstract translation: 描述了用于确定多个细胞上的药物效力的系统。 该系统包括使与多个生物细胞混合的铁液体流过药筒的入口部分，该药筒包括多个微流体通道，入口与多个通道中的每一个的一部分连通， 在所述入口部分和所述多个微通道中的至少一个附近施加磁场，其中所述磁场被配置为在所述混合物上施加间接力，当所述混合物流入所述混合物时，根据至少第一类型分离生物细胞 第一个方向 以及至少将所述第一类型的细胞引向通过至少一个所述微通道对受体进行功能化的第一传感器，所述传感器布置成邻近所述第一入口部分下游的至少一个所述微通道的第二部分。

公开(公告)号：US20180188246A1

公开(公告)日：2018-07-05

申请号：US15740288

申请日：2016-07-01

Applicant: Ancera, Inc.

Inventor： Hur KOSER

IPC: G01N33/543 ,  B03C1/023 ,  B03C1/033 ,  B03C1/28 ,  B03C1/32

CPC classification number: G01N33/54333 ,  B03C1/023 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/288 ,  B03C1/32 ,  B03C2201/18 ,  B03C2201/26

Abstract: Devices, methods, and systems are provided for extracting particles from a ferrofluid and for rapid affinity measurements. Such systems may comprise a fluidic channel or chamber configured to include a ferrofluid having a plurality of target particles and background particles. The systems may include a capture region configured to capture at least a portion of the plurality of target particles. In addition, the systems include a first magnetic field generator and a second magnetic field generator. The first magnetic field generator may be arranged proximate to the fluidic channel, the first magnetic field generator being configured to generate a first magnetic field configured to direct the plurality of target particles towards the capture region. The second magnetic field generator can be arranged to be proximate to the capture region, and is further configured to generate an affinity thresholding magnetic field configured to remove background particles from the capture region.

公开(公告)号：US20170285060A1

公开(公告)日：2017-10-05

申请号：US15623134

申请日：2017-06-14

Applicant: Ancera, Inc.

Inventor： Hur KOSER

IPC: G01N33/543 ,  B01L3/00 ,  B03C1/01 ,  B03C1/28 ,  B03C1/32 ,  G01N15/14 ,  G01N27/447 ,  G01N35/00

CPC classification number: G01N33/54366 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/288 ,  B03C1/32 ,  B03C2201/18 ,  B03C2201/20 ,  B03C2201/26 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N27/44786 ,  G01N33/54313 ,  G01N35/0098 ,  G01N2015/1415 ,  G01N2015/1486 ,  G01N2015/149

Abstract: Some embodiments of the present disclosure are directed to systems and methods for separating, directing, and/or extracting a target molecule from a mix of molecules and may comprise a plurality of non-magnetic beads suspended in a ferro fluid, where the non-magnetic beads may be functionalized with at least one predetermined first molecule configured to bind with a target particle. A microfluidic device may be included which may comprise at least one microfluidic channel, the device configured to dynamically and/or statically receive an amount of the mix. Magnetic field means may be included and may be configured to apply a magnetic field to at least a portion of the at least one channel to exert an indirect force on the non-magnetic heads in the ferro fluid mix, and separate the non-magnetic beads from the ferrofluid. The beads may then be directed to at least one receptor region. At least one outlet may be provided which is arranged to be in communication with the at least one microfluidic channel, the at least one outlet may be configured to receive and extract the separated non-magnetic beads from the ferrofluid.

公开(公告)号：US20160299052A1

公开(公告)日：2016-10-13

申请号：US14777511

申请日：2014-03-14

Applicant: ANCERA, INC.

Inventor： Hur KOSER

IPC: G01N15/10 ,  G01N33/543

CPC classification number: G01N15/1031 ,  G01N15/10 ,  G01N27/128 ,  G01N33/5438 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1075

Abstract: A device for determining the identity and concentration of target particles within a biocompatible ferrofluid medium is described. The system includes a fluidic channel that a sample of particles flow through; at least one magnetic field source configured to react repulsively with the particles; a channel wall with at least one receptor regions placed serially along the flow direction; and at least one thin electrode placed between the receptor regions to track changes in local impedance every time a target particle passes through the fluidic channel in close proximity.

Abstract translation: 描述了用于确定生物相容性铁磁流体介质内的靶颗粒的特性和浓度的装置。 该系统包括流体通道，颗粒样品流过; 至少一个磁场源被配置成与颗粒反应地反应; 通道壁，其具有沿流动方向串联放置的至少一个受体区域; 以及放置在受体区域之间的至少一个薄电极，以便每当目标颗粒通过紧邻的流体通道时跟踪局部阻抗的变化。

公开(公告)号：US20160296945A1

公开(公告)日：2016-10-13

申请号：US14777505

申请日：2014-03-14

Applicant: ANCERA, INC.

Inventor： Hur KOSER

IPC: B03C1/32 ,  C12N13/00 ,  C02F1/48 ,  B03C1/28 ,  C02F1/44

CPC classification number: B03C1/32 ,  B03C1/023 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/253 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  C02F1/44 ,  C02F1/488 ,  C12N13/00 ,  G01N35/0098

Abstract: A device and method for extracting particles contained in a ferrofluid medium are provided. Such methods may comprise suspending particles of different sizes in a ferrofluid medium and containing the ferrofluid medium in a cylindrical reservoir, and applying a first magnetic field to at least a portion of the reservoir. The first magnetic field is configured to indirectly exert a force on at least a portion of the particles of a predetermined size, and direct the portion of particles in a desired direction.

Abstract translation: 提供一种用于提取包含在铁磁介质中的颗粒的装置和方法。 这样的方法可以包括将不同尺寸的颗粒悬浮在铁磁流体介质中并将铁磁流体介质包含在圆柱形储存器中，以及向储存器的至少一部分施加第一磁场。 第一磁场被配置为间接地对至少一部分预定尺寸的颗粒施加力，并将颗粒的一部分引导到期望的方向。

公开(公告)号：US20160296944A1

公开(公告)日：2016-10-13

申请号：US14777504

申请日：2014-03-14

Applicant: ANCERA, INC.

Inventor： Hur KOSER

IPC: B03C1/28 ,  B01L3/00 ,  B03C1/033

CPC classification number: B03C1/288 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/043 ,  B03C1/0332 ,  B03C1/0335 ,  B03C2201/18 ,  B03C2201/26

Abstract: Systems, methods and devices are presented for extracting target particles within a ferrofluid medium. In some embodiments, a fluidic channel receives a flow of a mix of one or more types of target particles, where at least one magnetic field source is configured to react with the flow such that a force (indirect or direct) is placed on the particles of the mix, across the width and/or the height of the fluidic channel. An extraction opening placed on one wall is provided and configured to extract at least one type of target particle.

Abstract translation: 提出了用于提取铁磁流体介质中的目标颗粒的系统，方法和装置。 在一些实施例中，流体通道接收一种或多种类型的目标颗粒的混合物的流动，其中至少一个磁场源被配置为与流体反应，使得力（间接或直接）施加在颗粒上 的混合物，横跨流体通道的宽度和/或高度。 提供放置在一个壁上的提取开口并构造成提取至少一种类型的目标颗粒。