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    Compositions and methods for enhanced amino acid levels in plants conferred by lysine and/or threonine feedback insensitive aspartate kinase proteins
    4.
    发明授权
    Compositions and methods for enhanced amino acid levels in plants conferred by lysine and/or threonine feedback insensitive aspartate kinase proteins 有权
    赖氨酸和/或苏氨酸反馈不敏感天冬氨酸激酶蛋白质赋予植物氨基酸水平的组合物和方法

    公开(公告)号：US09090882B2

    公开(公告)日：2015-07-28

    申请号：US12997860

    申请日：2009-06-30

    申请人： James H. Crowley ,  Barry S. Goldman ,  Jintai Huang ,  Qungang Qi ,  William D. Rapp

    发明人： James H. Crowley ,  Barry S. Goldman ,  Jintai Huang ,  Qungang Qi ,  William D. Rapp

    IPC分类号： C12N9/12 ,  C12N15/82

    CPC分类号： C12N9/1217 ,  C12N15/8251 ,  C12N15/8254

    摘要： Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form β-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feedback-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.

    摘要翻译： 苏氨酸是人类和动物饲料工业中必不可少的氨基酸，其饲料配给水平可以显着影响重要肉源的生产成本，如猪和家禽。 苏氨酸以及必需氨基酸赖氨酸和甲硫氨酸均通过天冬氨酸家族途径合成。 天冬氨酸激酶（AK）是途径中的第一个酶，并催化天冬氨酸形成磷酸二酯的ATP依赖性磷酸化。 AK构成控制通过该途径的代谢通量的主要调节步骤，并且受到Lys和/或Thr的最终产物抑制。 本发明提供了一种通过在开发大豆植物和种子中反馈抗性AK酶的过表达产生转基因高游离苏氨酸大豆的方法。 这些修饰提供了增强植物氮代谢和作物生长性能的方法。

    Compositions and Methods for Enhanced Amino Acid Levels in Plants
    6.
    发明申请
    Compositions and Methods for Enhanced Amino Acid Levels in Plants 有权
    植物中增强的氨基酸水平的组成和方法

    公开(公告)号：US20110191898A1

    公开(公告)日：2011-08-04

    申请号：US12997860

    申请日：2009-06-30

    申请人： James H. Crowley ,  Barry S. Goldman ,  Jintai Huang ,  Qungang Qi ,  Willam D. Rapp

    发明人： James H. Crowley ,  Barry S. Goldman ,  Jintai Huang ,  Qungang Qi ,  Willam D. Rapp

    IPC分类号： C12N15/87 ,  C07H21/04 ,  C12N9/12 ,  C12N5/10 ,  A01H5/00

    CPC分类号： C12N9/1217 ,  C12N15/8251 ,  C12N15/8254

    摘要： Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form β-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feed-back-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.

    摘要翻译： 苏氨酸是人类和动物饲料工业中必不可少的氨基酸，其饲料配给水平可以显着影响重要肉源的生产成本，如猪和家禽。 苏氨酸以及必需氨基酸赖氨酸和甲硫氨酸均通过天冬氨酸家族途径合成。 天冬氨酸激酶（AK）是途径中的第一个酶，并催化天冬氨酸形成磷酸二酯的ATP依赖性磷酸化。 AK构成控制通过该途径的代谢通量的主要调节步骤，并且受到Lys和/或Thr的最终产物抑制。 本发明提供了一种在开发大豆植物和种子中通过反馈抗性AK酶的过表达产生转基因高游离苏氨酸大豆的方法。 这些修饰提供了增强植物氮代谢和作物生长性能的方法。