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公开(公告)号：US20110191898A1
公开(公告)日：2011-08-04
申请号：US12997860
申请日：2009-06-30
申请人： James H. Crowley , Barry S. Goldman , Jintai Huang , Qungang Qi , Willam D. Rapp
发明人： James H. Crowley , Barry S. Goldman , Jintai Huang , Qungang Qi , Willam D. Rapp
CPC分类号： C12N9/1217 , C12N15/8251 , C12N15/8254
摘要： Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form β-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feed-back-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.
摘要翻译： 苏氨酸是人类和动物饲料工业中必不可少的氨基酸，其饲料配给水平可以显着影响重要肉源的生产成本，如猪和家禽。 苏氨酸以及必需氨基酸赖氨酸和甲硫氨酸均通过天冬氨酸家族途径合成。 天冬氨酸激酶（AK）是途径中的第一个酶，并催化天冬氨酸形成磷酸二酯的ATP依赖性磷酸化。 AK构成控制通过该途径的代谢通量的主要调节步骤，并且受到Lys和/或Thr的最终产物抑制。 本发明提供了一种在开发大豆植物和种子中通过反馈抗性AK酶的过表达产生转基因高游离苏氨酸大豆的方法。 这些修饰提供了增强植物氮代谢和作物生长性能的方法。
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2.发明授权Compositions and methods for enhanced amino acid levels in plants conferred by lysine and/or threonine feedback insensitive aspartate kinase proteins 有权赖氨酸和/或苏氨酸反馈不敏感天冬氨酸激酶蛋白质赋予植物氨基酸水平的组合物和方法公开(公告)号：US09090882B2
公开(公告)日：2015-07-28
申请号：US12997860
申请日：2009-06-30
CPC分类号： C12N9/1217 , C12N15/8251 , C12N15/8254
摘要： Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form β-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feedback-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.
摘要翻译： 苏氨酸是人类和动物饲料工业中必不可少的氨基酸，其饲料配给水平可以显着影响重要肉源的生产成本，如猪和家禽。 苏氨酸以及必需氨基酸赖氨酸和甲硫氨酸均通过天冬氨酸家族途径合成。 天冬氨酸激酶（AK）是途径中的第一个酶，并催化天冬氨酸形成磷酸二酯的ATP依赖性磷酸化。 AK构成控制通过该途径的代谢通量的主要调节步骤，并且受到Lys和/或Thr的最终产物抑制。 本发明提供了一种通过在开发大豆植物和种子中反馈抗性AK酶的过表达产生转基因高游离苏氨酸大豆的方法。 这些修饰提供了增强植物氮代谢和作物生长性能的方法。
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公开(公告)号：US08357833B2
公开(公告)日：2013-01-22
申请号：US13042433
申请日：2011-03-07
申请人： Scott Andersen , James H. Crowley , Stephen M. Duff , Bradon J. Fabbri , Qungang Qi , Bo-Xing Qiu , Steven E. Screen
发明人： Scott Andersen , James H. Crowley , Stephen M. Duff , Bradon J. Fabbri , Qungang Qi , Bo-Xing Qiu , Steven E. Screen
IPC分类号： A01H1/00 , C12N15/52 , C07H21/04 , C07K14/415
CPC分类号： C12N15/8251 , C12N9/93
摘要： The present invention relates to a corn plant and seed with enhanced levels of protein and amino acids. The invention also relates to DNA constructs that provide expression in transgenic corn cells of an asparagine synthetase enzyme. The DNA constructs are used in a method to produce transgenic corn plants and seeds and to select for plants and seeds with enhanced levels of protein and amino acids.
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公开(公告)号：US08816156B2
公开(公告)日：2014-08-26
申请号：US13151082
申请日：2011-06-01
申请人： Andrew J. Brown , James F. Byrne , Robert H. Cole , James H. Crowley , John A. Miklos , Robert C. Ripley , Simone Seifert-Higgins , Jiali Xie
发明人： Andrew J. Brown , James F. Byrne , Robert H. Cole , James H. Crowley , John A. Miklos , Robert C. Ripley , Simone Seifert-Higgins , Jiali Xie
CPC分类号： C12N15/8275 , A01N57/20
摘要： The invention provides plants comprising transgenic event MON 88302 that exhibit tolerance to glyphosate herbicide. The invention also provides seeds, plant parts, cells, commodity products, and methods related to the event. The invention also provides DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a Brassica napus plant.
摘要翻译： 本发明提供了包含对草甘膦除草剂具有耐受性的转基因事件MON 88302的植物。 本发明还提供与事件有关的种子，植物部分，细胞，商品和方法。 本发明还提供了事件独有的DNA分子，并通过将转基因DNA插入到欧洲油菜植物的基因组中产生。
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公开(公告)号：US20090100536A1
公开(公告)日：2009-04-16
申请号：US11879785
申请日：2007-07-18
申请人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
发明人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
CPC分类号： C12N15/8279 , A01D45/00 , A01N25/34 , A01N31/16 , A01N41/10 , A01N43/40 , A01N43/653 , A01N43/78 , A01N43/80 , A01N55/02 , C07K14/415 , C12N9/0071 , C12N15/8241 , C12N15/8243 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12Y114/12017 , A01N25/10 , A01N25/02 , A01N2300/00
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。
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公开(公告)号：US20110302667A1
公开(公告)日：2011-12-08
申请号：US13151082
申请日：2011-06-01
申请人： Andrew J. Brown , James F. Byrne , Robert H. Cole , James H. Crowley , John A. Miklos , Robert C. Ripley , Simone Seifert-Higgins , Jiali Xie
发明人： Andrew J. Brown , James F. Byrne , Robert H. Cole , James H. Crowley , John A. Miklos , Robert C. Ripley , Simone Seifert-Higgins , Jiali Xie
IPC分类号： A01H5/00 , A01H5/10 , C12N5/10 , A01H5/02 , A01H5/06 , A01H5/04 , A01H5/12 , C11B1/00 , A01N57/20 , A01P13/00 , A01H1/02 , C12Q1/68 , A23L1/36 , A21D2/00 , A23K1/00 , C07H21/04
CPC分类号： C12N15/8275 , A01N57/20
摘要： The invention provides plants comprising transgenic event MON 88302 that exhibit tolerance to glyphosate herbicide. The invention also provides seeds, plant parts, cells, commodity products, and methods related to the event. The invention also provides DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a Brassica napus plant.
摘要翻译： 本发明提供了包含对草甘膦除草剂具有耐受性的转基因事件MON 88302的植物。 本发明还提供与事件有关的种子，植物部分，细胞，商品和方法。 本发明还提供了事件独有的DNA分子，并通过将转基因DNA插入到欧洲油菜植物的基因组中产生。
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公开(公告)号：US20130074202A1
公开(公告)日：2013-03-21
申请号：US13470467
申请日：2012-05-14
申请人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K. R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
发明人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K. R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
CPC分类号： C12N15/8279 , A01D45/00 , A01N25/34 , A01N31/16 , A01N41/10 , A01N43/40 , A01N43/653 , A01N43/78 , A01N43/80 , A01N55/02 , C07K14/415 , C12N9/0071 , C12N15/8241 , C12N15/8243 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12Y114/12017 , A01N25/10 , A01N25/02 , A01N2300/00
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
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公开(公告)号：US20110258734A1
公开(公告)日：2011-10-20
申请号：US13046601
申请日：2011-03-11
申请人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
发明人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
CPC分类号： C12N15/8279 , A01D45/00 , A01N25/34 , A01N31/16 , A01N41/10 , A01N43/40 , A01N43/653 , A01N43/78 , A01N43/80 , A01N55/02 , C07K14/415 , C12N9/0071 , C12N15/8241 , C12N15/8243 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12Y114/12017 , A01N25/10 , A01N25/02 , A01N2300/00
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。
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