-
1.发明申请Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol 审中-公开用于将木质纤维素生物质转化为乙醇的嗜热生物体公开(公告)号：US20090221049A1
公开(公告)日：2009-09-03
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。
-
公开(公告)号：US10066217B2
公开(公告)日：2018-09-04
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Kara Podkaminer , John Bardsley , David Anthony Hogsett
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
-
3.发明申请Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol 审中-公开用于将木质纤维素生物质转化为乙醇的嗜热生物体公开(公告)号：US20120077239A9
公开(公告)日：2012-03-29
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。
-
公开(公告)号：US20110177573A1
公开(公告)日：2011-07-21
申请号：US13055783
申请日：2009-08-03
申请人： Sarah All , David Anthony Hogsett
发明人： Sarah All , David Anthony Hogsett
IPC分类号： C12P7/06
摘要： Aspects of the present invention relate to methods of microbially treating lignocellulosic biomass using cellulose- and/or hemicellulose-degrading bacteria. In certain embodiments, the microbially treated material is then subjected to thermal and/or chemical pretreatment. In tandem with the microbial treatment the thermal and/or chemical pretreatment may result in the production of fewer degradation products, thereby allowing for higher overall yields of ethanol per ton of starting biomass.
摘要翻译： 本发明的方面涉及使用纤维素和/或半纤维素降解细菌微生物处理木质纤维素生物质的方法。 在某些实施方案中，微生物处理的材料然后进行热和/或化学预处理。 与微生物处理一起，热和/或化学预处理可能导致生产较少的降解产物，从而允许每吨起始生物质获得更高的乙醇总产率。
-
5.发明授权Gene knockout mesophilic and thermophilic organisms, and methods of use thereof 失效基因敲除嗜温和嗜热生物及其使用方法公开(公告)号：US08435770B2
公开(公告)日：2013-05-07
申请号：US12599458
申请日：2008-05-09
CPC分类号： C12P7/10 , C12N9/0006 , C12N9/1029 , C12N9/1205 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N9/92 , C12N15/74 , C12P7/065 , C12P2203/00 , C12Y101/01002 , C12Y101/01027 , C12Y101/02003 , C12Y203/01008 , C12Y207/01017 , C12Y207/02001 , C12Y503/01005 , Y02E50/16 , Y02E50/17
摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce ethanol as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of ethanol. Another aspect of the invention relates to a process for converting lignocellulosic biomass to ethanol, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.
摘要翻译： 本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，所述第一天然基因编码参与 有机酸或其盐的代谢产生，从而增加所述嗜热或嗜温微生物产生乙醇作为发酵产物的天然能力。 在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乙醇代谢产生的第一非天然酶。 本发明的另一方面涉及将木质纤维素生物质转化为乙醇的方法，包括使木质纤维素生物质与遗传修饰的嗜热或嗜温微生物接触。
-
6.发明申请GENE KNOCKOUT MESOPHILIC AND THERMOPHILIC ORGANISMS, AND METHODS OF USE THEREOF 失效基因克隆夫妇和热休克有机体及其使用方法公开(公告)号：US20100297721A1
公开(公告)日：2010-11-25
申请号：US12599458
申请日：2008-05-09
CPC分类号： C12P7/10 , C12N9/0006 , C12N9/1029 , C12N9/1205 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N9/92 , C12N15/74 , C12P7/065 , C12P2203/00 , C12Y101/01002 , C12Y101/01027 , C12Y101/02003 , C12Y203/01008 , C12Y207/01017 , C12Y207/02001 , C12Y503/01005 , Y02E50/16 , Y02E50/17
摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce ethanol as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of ethanol. Another aspect of the invention relates to a process for converting lignocellulosic biomass to ethanol, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.
摘要翻译： 本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，所述第一天然基因编码参与 有机酸或其盐的代谢产生，从而增加所述嗜热或嗜温微生物产生乙醇作为发酵产物的天然能力。 在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乙醇代谢产生的第一非天然酶。 本发明的另一方面涉及将木质纤维素生物质转化为乙醇的方法，包括使木质纤维素生物质与遗传修饰的嗜热或嗜温微生物接触。
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.031 秒)
