公开(公告)号：US20090221049A1

公开(公告)日：2009-09-03

申请号：US12299070

申请日：2007-05-01

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

IPC分类号： C12P7/06 ,  C12N1/21 ,  C12N15/11 ,  C12N15/00

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。

公开(公告)号：US20120077239A9

公开(公告)日：2012-03-29

申请号：US12299070

申请日：2007-05-01

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

IPC分类号： C12P7/06 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。

公开(公告)号：US10066217B2

公开(公告)日：2018-09-04

申请号：US12299070

申请日：2007-05-01

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

IPC分类号： C12N9/10 ,  C12N9/88 ,  C12N9/12 ,  C12P7/04 ,  C12P7/16 ,  C12N9/04 ,  C12P7/10 ,  C12R1/145

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

公开(公告)号：US20100015678A1

公开(公告)日：2010-01-21

申请号：US12090745

申请日：2006-10-31

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

IPC分类号： C12P7/10 ,  C12N1/21 ,  C12N15/11 ,  C12N15/00

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 两种生物体产生近乎理论乙醇产量而不表达丙酮酸脱羧酶

公开(公告)号：US20090239277A1

公开(公告)日：2009-09-24

申请号：US12398876

申请日：2009-03-05

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

IPC分类号： C12P7/06 ,  C12N1/21 ,  C12N15/11 ,  C12N15/00

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 两种生物体产生近乎理论乙醇产量而不表达丙酮酸脱羧酶

公开(公告)号：US20080280340A1

公开(公告)日：2008-11-13

申请号：US11579640

申请日：2005-05-06

申请人： Mikhail V. Tyurin ,  Lee R. Lynd ,  Charles R. Sullivan

发明人： Mikhail V. Tyurin ,  Lee R. Lynd ,  Charles R. Sullivan

IPC分类号： C12N13/00 ,  C12M1/00

CPC分类号： A61N1/0412 ,  A61N1/327 ,  C12M35/02 ,  C12N13/00

摘要： A method and apparatus for electroporation includes placing a mixture of bacterial suspension and transforming DNA into an electroporation cuvette. The resulting sample is subjected through a current-limiting device to a complex 5 waveform including a burst of high-voltage radio-frequency current, which in some embodiments is superimposed on a biphasic high-voltage DC pulse, and in other embodiments on a high-voltage lower-frequency AC burst. The total waveform has at least an initial portion greater than eleven thousand volts per centimeter of electrode spacing, and a later portion in some embodiments is reduced to less than thirty percent 10 of magnitude of the initial portion. Transformed bacteria are selected by culture in selective medium in an embodiment. The high-voltage radio-frequency current is between 3 and 125 MHz, and in an embodiment is 24 MHz.

摘要翻译： 用于电穿孔的方法和装置包括将细菌悬浮液和转化DNA的混合物放入电穿孔试管中。 将所得样品通过限流装置经受包括高压射频电流脉冲串的复合体5波形，其在一些实施例中叠加在双相高压DC脉冲上，而在其它实施例中为高电平 电压低频交流脉冲串。 总波形具有至少一个初始部分大于每千米电极间距大于11000伏特，并且在一些实施例中，稍后的部分被减小到初始部分大小的百分之十。 在一个实施方案中，通过在选择培养基中培养来选择转化的细菌 高压射频电流在3和125MHz之间，在一个实施例中是24MHz。

公开(公告)号：US08394611B2

公开(公告)日：2013-03-12

申请号：US12226763

申请日：2007-04-30

申请人： Bruce E. Dale ,  Lee R. Lynd ,  Mark Laser

发明人： Bruce E. Dale ,  Lee R. Lynd ,  Mark Laser

IPC分类号： C12P19/02 ,  C12P7/02 ,  C12P19/00 ,  C13K1/02 ,  C01C1/00

CPC分类号： C12P19/02 ,  C12P7/10 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  Y02E50/16

摘要： A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.

摘要翻译： 描述了使用具有或不具有无水氨添加的浓氢氧化铵来处理生物质以使结构碳水化合物更易于和/或消化的方法。 该方法优选使用蒸汽从生物质中除去氨以进行再循环。 来自结构碳水化合物的单糖的方法产率是好的，特别是通过结构碳水化合物的酶水解测量。 单糖用作乙醇生产的动物饲料和能源。

公开(公告)号：US20120264183A1

公开(公告)日：2012-10-18

申请号：US13498861

申请日：2010-09-28

申请人： Maria Sizova ,  Javier Izquierdo ,  Lee R. Lynd

发明人： Maria Sizova ,  Javier Izquierdo ,  Lee R. Lynd

IPC分类号： C12N1/20 ,  C12P7/54 ,  C12N9/24 ,  C12P7/10 ,  C12P7/06 ,  C12N1/21 ,  C12P7/56 ,  C12P7/40

CPC分类号： C12N9/2405 ,  C12N1/20 ,  C12P7/065 ,  C12P7/10 ,  C12P7/54 ,  C12R1/145 ,  Y02E50/16 ,  Y02E50/17

摘要： A new species of an anaerobic thermophilic cellulolytic and xylano lytic bacterium is disclosed. One particular strain of this new species has been deposited with the ATCC under Deposit No. PTA-10114. It is also provided a method for isolating, culturing and utilizing this novel bacterium for the conversion of biomass to bioconversion products, such as ethanol.

摘要翻译： 公开了一种新型的厌氧嗜热纤维素分解和木糖溶解细菌。 这种新物种的一种特定菌株已经保藏在保藏号PTA-10114的ATCC。 还提供了用于分离，培养和利用这种用于将生物质转化为生物转化产物如乙醇的新型细菌的方法。

公开(公告)号：US20110244539A1

公开(公告)日：2011-10-06

申请号：US13121383

申请日：2009-09-28

申请人： Nicolai Panikov ,  Maria Sizova ,  Lee R. Lynd

发明人： Nicolai Panikov ,  Maria Sizova ,  Lee R. Lynd

IPC分类号： C12P7/10 ,  C12N1/20

CPC分类号： C12N15/01 ,  C12N1/20 ,  C12N1/22 ,  C12P7/10 ,  C12R1/145 ,  Y02E50/16 ,  Y02E50/17

摘要： Methods for obtaining cellulolytic microbes with high growth rates are disclosed. For example, C. thermocellum colonies with growth rates higher than 0.17 hr″ 1 have been obtained by the present methods. In realizing higher growth rates, better bioprocessing efficiency can be achieved resulting in increased economy.

摘要翻译： 公开了获得高生长速度的纤维素分解微生物的方法。 例如，通过本发明的方法已经获得了生长速率高于0.17小时-1的热纤维梭菌菌落。 在实现更高增长率时，可以实现更好的生物处理效率，从而增加经济效益。

公开(公告)号：US20130302870A1

公开(公告)日：2013-11-14

申请号：US13704122

申请日：2011-06-20

申请人： Lee R. Lynd ,  Chaogong Liu ,  David A. Hogsett

发明人： Lee R. Lynd ,  Chaogong Liu ,  David A. Hogsett

IPC分类号： B01D61/14

CPC分类号： B01D61/14 ,  C08H8/00 ,  C10G1/002 ,  C10G1/02 ,  C10G1/04 ,  C10G2300/1011 ,  C10G2300/1014 ,  C10G2300/807 ,  C10L1/023 ,  C12P7/10 ,  C12P2201/00 ,  Y02E50/16 ,  Y02P30/20

摘要： A new and improved biomass conversion system is disclosed using high-temperature flow-though pretreatment and a nanoporous membrane to provide more digestible biomass for subsequent conversion to biofuels.

摘要翻译： 公开了一种新的和改进的生物质转化系统，其使用高温流通预处理和纳米多孔膜以提供更可消化的生物质用于随后转化为生物燃料。