公开(公告)号：US20120077239A9

公开(公告)日：2012-03-29

申请号：US12299070

申请日：2007-05-01

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

IPC分类号： C12P7/06 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。

公开(公告)号：US20100015678A1

公开(公告)日：2010-01-21

申请号：US12090745

申请日：2006-10-31

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

IPC分类号： C12P7/10 ,  C12N1/21 ,  C12N15/11 ,  C12N15/00

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 两种生物体产生近乎理论乙醇产量而不表达丙酮酸脱羧酶

公开(公告)号：US20090239277A1

公开(公告)日：2009-09-24

申请号：US12398876

申请日：2009-03-05

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin

IPC分类号： C12P7/06 ,  C12N1/21 ,  C12N15/11 ,  C12N15/00

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 两种生物体产生近乎理论乙醇产量而不表达丙酮酸脱羧酶

公开(公告)号：US20090221049A1

公开(公告)日：2009-09-03

申请号：US12299070

申请日：2007-05-01

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Mikhail V. Tyurin ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

IPC分类号： C12P7/06 ,  C12N1/21 ,  C12N15/11 ,  C12N15/00

CPC分类号： C12N9/1029 ,  C12N9/0006 ,  C12N9/1217 ,  C12P7/10 ,  C12R1/145 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

摘要翻译： 本文公开了消耗多种生物质衍生底物的突变嗜热生物。 本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。 此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。 涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。 生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。

公开(公告)号：US10066217B2

公开(公告)日：2018-09-04

申请号：US12299070

申请日：2007-05-01

申请人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

发明人： Arthur Josephus Shaw, IV ,  Sunil G. Desai ,  Lee R. Lynd ,  Kara Podkaminer ,  John Bardsley ,  David Anthony Hogsett

IPC分类号： C12N9/10 ,  C12N9/88 ,  C12N9/12 ,  C12P7/04 ,  C12P7/16 ,  C12N9/04 ,  C12P7/10 ,  C12R1/145

摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

公开(公告)号：US20110256601A1

公开(公告)日：2011-10-20

申请号：US12808764

申请日：2008-12-17

申请人： Arthur Josephus Shaw, IV ,  Lee Lynd ,  David A. Hogsett

发明人： Arthur Josephus Shaw, IV ,  Lee Lynd ,  David A. Hogsett

IPC分类号： C12P7/10 ,  C12N1/19 ,  C12N9/02 ,  C12P7/06 ,  C12N1/20 ,  C07H21/04 ,  C12N1/21 ,  C12N15/63

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0067 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

摘要： Bacteria consume a variety of biomass-derived substrates and produce ethanol. Hydrogenase genes have been inactivated m Thermoanaerobacterium saccharolyticum to generate mutant strains with reduced hydrogenase activities. One such mutant strain with both the ldh and hydtrA genes inactivated shows a significant increase in ethanol production. Manipulation of hydrogenase activities provides a new approach for enhancing substrate utilization and ethanol production by biomass-fermenting microorganisms.

摘要翻译： 细菌消耗各种生物质来源的底物并产生乙醇。 氢解酶基因已被灭活，从而产生具有降低的氢化酶活性的突变菌株。 一个这样的具有ldh和hydtrA基因失活的这种突变菌株显示出乙醇生产的显着增加。 氢化酶活性的操作提供了生物质发酵微生物增强底物利用和乙醇生产的新方法。