公开(公告)号：US08367393B2

公开(公告)日：2013-02-05

申请号：US13130729

申请日：2009-11-20

申请人： Bärbel Hahn-Hägerdal ,  Oskar Bengtsson ,  Maurizio Bettiga ,  Rosa Garcia Sanchez ,  David Rundquist ,  Marie-Francoise Gorwa-Grauslund

发明人： Bärbel Hahn-Hägerdal ,  Oskar Bengtsson ,  Maurizio Bettiga ,  Rosa Garcia Sanchez ,  David Rundquist ,  Marie-Francoise Gorwa-Grauslund

IPC分类号： C12N1/20

CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/90 ,  C12P7/06 ,  C12Y101/01009 ,  Y02E50/17

摘要： The invention relates to an improved Saccharomyces strain displaying improved viability and growth during anaerobic fermentation of pentose carbon sources such as xylose and producing fermentation products such as ethanol.

摘要翻译： 本发明涉及一种改良的酵母属菌株，其在戊糖碳源（如木糖）的厌氧发酵过程中显示出改善的活力和生长，并产生发酵产物如乙醇。

公开(公告)号：US20110294180A1

公开(公告)日：2011-12-01

申请号：US13130729

申请日：2009-11-20

申请人： Bärbel Hahn-Hägerdal ,  Oskar Bengtsson ,  Maurizio Bettiga ,  Rosa Garcia Sanchez ,  David Rundquist ,  Marie-Francoise Gorwa-Grauslund

发明人： Bärbel Hahn-Hägerdal ,  Oskar Bengtsson ,  Maurizio Bettiga ,  Rosa Garcia Sanchez ,  David Rundquist ,  Marie-Francoise Gorwa-Grauslund

IPC分类号： C12P7/06 ,  C12N1/19

CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/90 ,  C12P7/06 ,  C12Y101/01009 ,  Y02E50/17

摘要： The invention relates to an improved Saccharomyces strain displaying improved viability and growth during anaerobic fermentation of pentose carbon sources such as xylose and producing fermentation products such as ethanol.

摘要翻译： 本发明涉及一种改良的酵母属菌株，其在戊糖碳源（如木糖）的厌氧发酵过程中显示出改善的活力和生长，并产生发酵产物如乙醇。

公开(公告)号：US20070155000A1

公开(公告)日：2007-07-05

申请号：US11560490

申请日：2006-11-16

申请人： Anneli Nilsson ,  Gunnar Lidén ,  Marie-Francoise Gorwa-Grauslund ,  Bärbel Hahn-Hägerdal ,  Carl Modig ,  Joao Moreira De Almeida

发明人： Anneli Nilsson ,  Gunnar Lidén ,  Marie-Francoise Gorwa-Grauslund ,  Bärbel Hahn-Hägerdal ,  Carl Modig ,  Joao Moreira De Almeida

IPC分类号： C12P7/06 ,  C12N1/18

CPC分类号： C12N9/0006 ,  C12N9/1205 ,  C12N9/92 ,  C12P7/10 ,  C12P17/04 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention relates to an ethanol producing microbial strain, such as Saccharomyces cerevisiae strain, being able to grow and produce ethanol from lignocellulosic hydrolysates comprising growth inhibiting compounds of the group furfural and 5-hydroxy-methyl furfural, in a batch, fed-batch or continuous fermentation, said microbial strain being tolerant to such inhibiting compounds, which strain is upregulated and/or over expressed with regard to one or more of the following genes: LAT1, ALD6, ADH5, ADH6, GDH3, OYE3, SER3, GND2, MDH2, IDP3, ADH7, AAD15, ERG27, HMG1, LYS5, SPS19, SGE1.

摘要翻译： 本发明涉及一种生产乙醇的微生物菌株，例如酿酒酵母菌株，能够从包含糠醛和5-羟甲基糠醛的生长抑制化合物的木质纤维素水解产物中分批生产和生产乙醇，补料分批 或连续发酵，所述微生物菌株对这种抑制化合物是耐受的，该菌株对于以下一个或多个基因：上调和/或过度表达：LAT1，ALD6，ADH5，ADH6，GDH3，OYE3，SER3，GND2， MDH2，IDP3，ADH7，AAD15，ERG27，HMG1，LYS5，SPS19，SGE1。

公开(公告)号：US20130023021A1

公开(公告)日：2013-01-24

申请号：US13545301

申请日：2012-07-10

申请人： Anneli Nilsson ,  Gunnar Lidén ,  Marie-Francoise Gorwa-Grauslund ,  Bärbel Hahn-Hägerdal ,  Carl Tobias Modig ,  Joao Ricardo Moreira De Almeida

发明人： Anneli Nilsson ,  Gunnar Lidén ,  Marie-Francoise Gorwa-Grauslund ,  Bärbel Hahn-Hägerdal ,  Carl Tobias Modig ,  Joao Ricardo Moreira De Almeida

IPC分类号： C12P7/10 ,  C12N1/16

CPC分类号： C12N9/0006 ,  C12N9/1205 ,  C12N9/92 ,  C12P7/10 ,  C12P17/04 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention relates to an ethanol producing microbial strain, such as Saccharomyces cerevisiae strain, being able to grow and produce ethanol from lignocellulosic hydrolysates comprising growth inhibiting compounds of the group furfural and 5-hydroxy-methyl furfural, in a batch, fed-batch or continuous fermentation, said microbial strain being tolerant to such inhibiting compounds, which strain is upregulated and/or over expressed with regard to one or more of the following genes: LAT1, ALD6, ADH5, ADH6, GDH3, OYE3, SER3, GND2, MDH2, IDP3, ADH7, AAD15, ERG27, HMG1, LYS5, SPS19, SGE1.

公开(公告)号：US07531348B2

公开(公告)日：2009-05-12

申请号：US10293255

申请日：2002-11-14

申请人： Ricardo Roman Cordero Otero ,  Bärbel Hahn-Hägerdal ,  Willhem Herber Van Zyl

发明人： Ricardo Roman Cordero Otero ,  Bärbel Hahn-Hägerdal ,  Willhem Herber Van Zyl

IPC分类号： C12N1/14 ,  C12N9/02 ,  C12N9/04 ,  C12N9/12 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N9/00 ,  C12N15/01 ,  C12Q1/00 ,  C12Q1/68 ,  C12P21/04

CPC分类号： C12N9/0006 ,  C12N9/1205 ,  C12P7/10 ,  Y02E50/16

摘要： The present invention relates to a method for obtaining a recombinant yeast of Saccharomyces cerevisiae, which ferments lignocellulose raw materials to ethanol, including introducing DNA into a yeast so as to cause the yeast to have introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.

摘要翻译： 本发明涉及一种获得酿酒酵母重组酵母的方法，其将木质纤维素原料发酵成乙醇，包括将DNA引入酵母中，以使酵母引入编码木糖还原酶，木糖醇脱氢酶和木酮糖激酶的基因。

公开(公告)号：US06475768B1

公开(公告)日：2002-11-05

申请号：US09545569

申请日：2000-04-07

申请人： Ricardo Román Cordero Otero ,  Márk Gárdonyi ,  Bärbel Hahn-Hägerdal ,  Willem Heber van Zyl ,  Eva Anna Viktoria Dackehag

发明人： Ricardo Román Cordero Otero ,  Márk Gárdonyi ,  Bärbel Hahn-Hägerdal ,  Willem Heber van Zyl ,  Eva Anna Viktoria Dackehag

IPC分类号： C12N990

CPC分类号： C12Y503/01005 ,  C12N9/92

摘要： The present invention relates to novel nucleic acid sequences which, upon expression in a procaryotic or eucaryotic cell, code for a polypeptide having an elevated specific xylose isomerase activity compared to the wildtype Thermus thermophilus xylose isomerase. They are selected from a) nucleic acid sequences shown in SEQ. ID. No 1; b) the complementary strand of the sequence defined in (a) above; c) nucleic acid sequences which hybridize to the sequences defined in (a) or (b) above; d) nucleic acid sequences which, but for the degeneracy of the genetic code, would hybridize to the sequences defined in (a), (b) or (c) above and which code for the same polypeptide as those defined in (a), (b) or (c) above. The present invention further provides a process of producing ethanol from xylose containing materials comprising contacting cells that express such nucleic acid sequences and novel modified xylose isomerases that advantageously can be applied in the production of fructose syrups.

摘要翻译： 本发明涉及新颖的核酸序列，其在原核或真核细胞中表达时编码与野生型嗜热栖热菌嗜热木糖木糖异构酶相比具有升高的特异性木糖异构酶活性的多肽。 它们选自a）SEQ ID NO： ID。 没有1; b）上文（a）中定义的序列的互补链; c）与上述（a）或（b）中定义的序列杂交的核酸序列; d）核酸序列，但是对于遗传密码的简并性，其将与上述（a），（b）或（c）中定义的序列杂交，并且与（a）中定义的相同多肽的编码， （b）或（c）。 本发明进一步提供了一种从含有木糖的材料生产乙醇的方法，包括使表达这种核酸序列的细胞接触和有利地可用于果糖糖浆生产中的新型修饰的木糖异构酶。

公开(公告)号：US06582944B1

公开(公告)日：2003-06-24

申请号：US09184965

申请日：1999-01-08

申请人： Johan Hallborn ,  Merja Penttilä ,  Heikki Ojamo ,  Mats Walfridsson ,  Ulla Airaksinen ,  Sirkka Keränen ,  Bärbel Hahn-Hägerdal

发明人： Johan Hallborn ,  Merja Penttilä ,  Heikki Ojamo ,  Mats Walfridsson ,  Ulla Airaksinen ,  Sirkka Keränen ,  Bärbel Hahn-Hägerdal

IPC分类号： C12P706

CPC分类号： C12P19/02 ,  C12N9/0004 ,  C12N9/0006 ,  C12N15/52 ,  C12N15/81 ,  C12N15/905 ,  C12P7/06 ,  C12P7/18 ,  C12P7/40 ,  Y02E50/17 ,  Y02P20/582

摘要： This invention relates to recombinant-DNA-technology. Specifically this invention relates to new recombinant yeast strains transformed with xylose reductase and/or xylitol dehydrogenase enzyme genes. A yeast strain transformed with the xylose reductase gene is capable of reducing xylose to xylitol and consequently of producing xylitol in vivo. If both of these genes are transformed into a yeast strain, the resultant strain is capable of producing ethanol on xylose containing medium during fermentation. Further, the said new yeast strains are capable of expressing the said two enzymes. Xylose reductase produced by these strains can be used in an enzymatic process for the production of xylitol in vitro.

摘要翻译： 本发明涉及重组DNA技术。 具体地说，本发明涉及用木糖还原酶和/或木糖醇脱氢酶基因转化的新的重组酵母菌株。 用木糖还原酶基因转化的酵母菌株能够将木糖还原成木糖醇，从而在体内产生木糖醇。 如果将这两个基因转化成酵母菌株，则所得菌株能够在发酵期间在含有木糖的培养基上产生乙醇。 此外，所述新酵母菌株能够表达所述两种酶。 由这些菌株产生的木糖还原酶可用于体外生产木糖醇的酶法。