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公开(公告)号：US10344317B2
公开(公告)日：2019-07-09
申请号：US15518760
申请日：2014-10-13
发明人： Hongyan Han , Chunyu Geng , Guanying Guo , Wenwei Zhang , Hui Jiang , Yuan Jiang
IPC分类号： C12P19/34 , C12Q1/6806 , C12Q1/6855 , C12Q1/686
摘要： Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5′-end extension primer, wherein the single-stranded 5′-end extension primer comprises a sequencing platform adaptor sequence of a 5′ end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3′-end adaptor sequence to the 3′ end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.
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2.发明授权公开(公告)号：US10087216B2
公开(公告)日：2018-10-02
申请号：US15515696
申请日：2014-09-30
发明人： Zhilong Lin , Bo Wen , Ting Tong , Jie Liu , Chaoqin Du , Fen Mo , Chao Peng , Qiong Shi
摘要： Disclosed are a conotoxin polypeptide κ-CPTx-bt101, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 18 amino acids, has a molecular weight of 1872.72 daltons, and has the full sequence KCCTMSVCQPPPVCTCCA (SEQ. ID NO. 1).
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公开(公告)号：US10023906B2
公开(公告)日：2018-07-17
申请号：US15510904
申请日：2014-10-14
发明人： Chunyu Geng , Rongrong Guo , Ruoying Chen , Lingyu He , Wenwei Zhang , Hui Jiang
IPC分类号： C12Q1/68 , C12N9/22 , C12Q1/6853 , C12N15/10 , C40B40/08 , C12N11/06 , C12N9/00 , C12Q1/686 , C40B50/06 , C40B50/14 , C12Q1/6806 , C12N15/66
CPC分类号： C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
摘要： Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5′ end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
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公开(公告)号：US20180195060A1
公开(公告)日：2018-07-12
申请号：US15567963
申请日：2016-04-14
发明人： Ou WANG , Cankun CHANG , Lin LIN , Hui JIANG , Wenwei ZHANG
CPC分类号： C12N15/1093 , C12N15/10 , C40B40/06 , C40B50/06 , C12Q2525/191 , C12Q2563/179
摘要： Disclosed is a method for constructing a long fragment DNA library, comprising the following steps: 1) breaking a long fragment DNA into target fragments of 3-10 kb by transposase, then amplifying the target fragments, and obtaining target fragment amplification products containing dUTP; 2) amplifying the dUTP in the products by removing the target fragments, fragmenting the target fragments secondarily into DNA short fragments of 300-1200 bp; 3) connecting both ends of the DNA short fragments with sequencing linker single chains A and sequencing linker single chains B respectively; and obtaining connecting sequencing linker products; and 4) PCR amplifying the connecting sequencing linker products, to obtain amplification products.
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公开(公告)号：US09890375B2
公开(公告)日：2018-02-13
申请号：US15510877
申请日：2014-09-12
发明人： Chunyu Geng , Dennis G. Ballinger , Yanyan Zhang , Shujin Fu , Lingyu He , Wenwei Zhang , Hui Jiang
CPC分类号： C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
摘要： Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5′-end nucleotide of the first strand has a phosphate group, and the 3′-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5′-end nucleotide of the second strand does not have a phosphate group, and the 3′-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.
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6.发明申请公开(公告)号：US20170349893A1
公开(公告)日：2017-12-07
申请号：US15529867
申请日：2014-11-26
发明人： Yuan JIANG , Xia ZHAO , Andrei ALEXEEV , Radoje DRMANAC , Wenwei ZHANG , Hui JIANG
IPC分类号： C12N15/10
摘要： A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase is provided on a primer sequence; using USER enzyme to cleave the first product and cyclizing to produce a gap; or, a nicking enzyme recognition sequence is also provided on the primer sequence, using the USER enzyme to cleave the first product, cyclizing and using a nicking enzyme for nicking to produce a nick; performing a restrictive nick/gap translation reaction from the nick or the gap; removing by digestion any portion that did not undergo the restrictive nick/gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and using a mediated sequence for cyclization of a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments and obviates the need for gel extraction; the single-strand nucleic acid molecule can be cyclized directly when denatured with heat.
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7.发明申请公开(公告)号：US20170305979A1
公开(公告)日：2017-10-26
申请号：US15515687
申请日：2014-09-30
发明人： Zhilong Lin , Bo Wen , Ting Tong , Jie Liu , Chaoqin Du , Fen Mo , Chao Peng , Qiong Shi
IPC分类号： C07K14/435 , C07H21/04 , C12N15/09 , C07K14/00 , A61K38/00
CPC分类号： C07K14/43504 , A61K38/00 , A61K38/10 , C07H21/04 , C07K1/00 , C07K7/08 , C07K14/00 , C12N15/09
摘要： Disclosed are a conotoxin polypeptide κ-CPTx-bt102, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 15 amino acids, has a molecular weight of 1660.61 daltons, and has the full sequence RCRCEQTCGTCVPCC (SEQ. ID NO. 1).
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8.发明申请公开(公告)号：US20170298103A1
公开(公告)日：2017-10-19
申请号：US15515658
申请日：2014-09-30
发明人： Zhilong Lin , Bo Wen , Ting Tong , Jie Liu , Chaoqin Du , Fen Mo , Chao Peng , Qiong Shi
IPC分类号： C07K14/435 , C07H21/04 , C12N15/09 , C07K14/00 , A61K38/00
CPC分类号： C07K14/43504 , A61K38/00 , C07H21/04 , C07K1/14 , C07K1/18 , C07K7/08 , C07K14/00 , C12N15/09
摘要： Disclosed are a conotoxin polypeptide κ-CPTx-bt105, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 16 amino acids, has a molecular weight of 1626.62 daltons, and has the full sequence GICCVDDTCTTHSGCL (SEQ. ID NO. 1).
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公开(公告)号：US20170275609A1
公开(公告)日：2017-09-28
申请号：US15510877
申请日：2014-09-12
发明人： Chunyu Geng , Dennis G. Ballinger , Yanyan Zhang , Shujin Fu , Lingyu He , Wenwei Zhang , Hui Jiang
CPC分类号： C12Q1/6853 , B01J19/0046 , B01J2219/00529 , C12N9/22 , C12N9/93 , C12N11/06 , C12N15/10 , C12N15/1065 , C12N15/1082 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q1/6811 , C12Q1/6837 , C12Q1/6855 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2565/537 , C40B40/06 , C40B40/08 , C40B50/06 , C40B50/14 , C40B60/14 , C12Q2521/525 , C12Q2535/122 , C12N15/1089 , C12Q2525/186 , C12Q2521/101
摘要： Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5′-end nucleotide of the first strand has a phosphate group, and the 3′-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5′-end nucleotide of the second strand does not have a phosphate group, and the 3′-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.
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公开(公告)号：US20170227528A1
公开(公告)日：2017-08-10
申请号：US15515501
申请日：2014-09-30
发明人： Qiang Feng , Zhipeng Liu , Nan Meng , Jun Wang
CPC分类号： G01N33/5091 , G01N30/02 , G01N30/62 , G01N30/72 , G01N33/48 , G01N2030/027 , G01N2800/324
摘要： The present invention relates to a disease-specific metabolite profile, and particularly to a biomarker composition obtained by screening from blood plasma-specific profiles of coronary heart disease subjects. The present invention also relates to a use of the biomarker compositions in risk assessment, diagnosis, early diagnosis, or pathological staging of coronary heart disease, and to a method for risk assessment, diagnosis, early diagnosis, or pathological staging of coronary heart disease. The biomarker composition as provided by the present invention can be used for early diagnosis of coronary heart disease and has high sensitivity, good specificity and good application prospects.
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