METHODS AND MICROORGANISMS FOR INCREASING THE BIOLOGICAL SYNTHESIS OF DIFUNCTIONAL ALKANES
    1.
    发明申请
    METHODS AND MICROORGANISMS FOR INCREASING THE BIOLOGICAL SYNTHESIS OF DIFUNCTIONAL ALKANES 审中-公开
    增加生物合成功能的方法和微生物

    公开(公告)号:US20130288320A1

    公开(公告)日:2013-10-31

    申请号:US13868213

    申请日:2013-04-23

    申请人: BIOAMBER INC.

    发明人: Man Kit Lau

    IPC分类号: C12P7/44

    摘要: A method of increasing the production a difunctional alkane in a microorganism that produces a difunctional alkane from alpha-keto acid by increasing the production of homocitrate in the cell relative to a wild-type or parent cell. The production of homocitrate may be obtained by engineering pathways that increase the production of alpha-ketoacid, such as alpha-ketoglutarate.

    摘要翻译: 通过增加细胞中相对于野生型或亲代细胞的高柠檬酸盐的产生,增加由α-酮酸产生双官能烷烃的微生物中的双官能烷烃的生产方法。 高产柠檬酸盐的生产可以通过增加α-酮酸生产的工程途径获得,例如α-酮戊二酸。

    Methods and Microorganisms for the Biological Synthesis of (S) -2-amino-6-hydroxypimelate, Hexamethylenediamine and 6-aminocaproate
    3.
    发明申请
    Methods and Microorganisms for the Biological Synthesis of (S) -2-amino-6-hydroxypimelate, Hexamethylenediamine and 6-aminocaproate 有权
    (S)-2-氨基-6-羟基庚酸酯,六亚甲基二胺和6-氨基己酸的生物合成的方法和微生物

    公开(公告)号:US20150203879A1

    公开(公告)日:2015-07-23

    申请号:US14419310

    申请日:2013-08-21

    申请人: BioAmber Inc.

    发明人: Man Kit Lau

    摘要: A chemical having the formula (S) 2-amino-6-hydroxypimelate. (S)-2-amino-6-hydroxypimelate can be made using a method comprising culturing a cell comprising an exogenous nucleic acid sequence encoding an enzyme that catalyzes the substrate to product conversion of (S)-2-amino-6-oxopimelate to (S)-2-amino-6-hydroxypimelate and separating the (S)-2-amino-6-hydroxypimelate. The cell may be a recombinant microorganism for producing aminocaproic acid or hexamethylenediamine from (S)-2-amino-6-hydroxypimelate comprising at least one exogenous nucleic acid sequence that expresses at least one polypeptide with substrate preference for homolysine, and amino acid decarboxylase with substrate preference for alpha-aminopimelate.

    摘要翻译: 具有式(S)2-氨基-6-羟基庚酸酯的化学式。 (S)-2-氨基-6-羟基庚二酸酯可以使用包括培养包含编码催化底物的酶的外源核酸序列的细胞的方法制备(S)-2-氨基-6-氧代庚酸成分转化为 (S)-2-氨基-6-羟基庚酸酯并分离(S)-2-氨基-6-羟基庚酸酯。 细胞可以是用于从(S)-2-氨基-6-羟基庚二酸生产氨基己酸或六亚甲基二胺的重组微生物,其包含至少一种外源核酸序列,其表达至少一种具有底物偏爱高赖氨酸的多肽,以及氨基酸脱羧酶与 底物优选α-氨基庚二酸酯。