公开(公告)号：US3762609A

公开(公告)日：1973-10-02

申请号：US3762609D

申请日：1972-06-12

申请人： BOEHRINGER MANNHEIM GMBH

发明人： HAGEN A ,  MEWES D ,  GRUBER W ,  BERGMEYER H

IPC分类号： G01N1/00 ,  G05D11/00 ,  B67D5/54

CPC分类号： G01N35/1097 ,  G05D11/001 ,  G05D11/003

摘要： A sample to be analyzed is injected into a stream of carrier liquid by coupled dual valves in two branches of the stream. One of the two valves is open and the other closed at any one time. The bore of the closed valve provides a metering cavity which is filled with the sample to be analyzed, and the sample is injected by simultaneously closing the previously open valve and opening the sample-bearing closed valve. The specific, dual valve arrangement disclosed has a single movable disc arranged between two stationary discs, the discs being provided with passages filled and emptied in the necessary sequence when the movable disc is rotated.

摘要翻译： 待分析的样品通过在流的两个分支中的耦合双阀注入载液流中。 两个阀门中的一个是打开的，另一个在任何一个时间都关闭。 关闭阀的孔提供了一个填充有待分析样品的计量腔，并且通过同时关闭先前打开的阀并打开带样本的封闭阀注入样品。 所公开的具体的双阀装置具有设置在两个固定盘之间的单个可移动盘，当可移动盘旋转时，盘设置有以必要顺序填充和排空的通道。

公开(公告)号：US3838011A

公开(公告)日：1974-09-24

申请号：US26176372

申请日：1972-06-12

申请人： BOEHRINGER MANNHEIM GMBH

发明人： HAGEN A ,  BERGMEYER H ,  GRUBER W ,  BEAUCAMP K ,  JAWOREK D

IPC分类号： G01N27/48 ,  B01L99/00 ,  C12Q1/00 ,  G01N33/487 ,  G01N31/14

CPC分类号： B01L3/0293 ,  C12Q1/00 ,  C12Q1/001 ,  Y10T137/86775 ,  Y10T436/117497

摘要： ENZYMATICALLY REACTIVE SUBSTANCES CONTAINED IN AQUEOUS SOLUTIONS ARE QUANTITATIVELY DETERMINED BY INTRODUCING A DEFINED AMOUNT OF A SOLUTION SAMPLE CONTAINING THE SUBSTANCE TO BE DETERMINED AND WHICH IS FREE OF AIR BUBBLES, INTO A STREAM OF BUFFER SOLUTION IN A BLOCK-LIKE FASHION, WHEREIN THE BUFFER SOLUTION CONTAINS THE REAGENTS NECESSARY FOR AN ENZYMATIC REACTION WITH SAID SUBSTANCES AND FLOWS WITH CONSTANT VELOCITY AND WHEREIN THE INTRODUCTION OF THE BLOCK SAMPLE DOES NOT SUBSTANTIALLY ALTER THE VELOCITY OF FLOW, CONTACTING THE RESULTING STREAM WITH THE APPROPRIATE ENZYME AND THEN PASSING THE STREAM OVER A DETECTOR TO INDICATE THE AMOUNT OF REACTION HAVING OCCURED, AND RELATING READINGS FROM THE DETECTOR TO THE AMOUNT OF THE SUBSTANCE TO BE DETERMINED CONTAINED IN THE SAMPLE.