Rhizobial diagnostic probes and rhizobium trifolii nifH promoters
    1.
    发明授权
    Rhizobial diagnostic probes and rhizobium trifolii nifH promoters 失效
    根瘤菌诊断探针和根瘤菌trifolii nifH启动子

    公开(公告)号:US5137816A

    公开(公告)日:1992-08-11

    申请号:US701358

    申请日:1991-05-10

    摘要: This invention provides useful promoters from the R. trifolii nifH gene for the construction of recombinant molecules to regulate foreign genes for expression under desired conditions. In particular, the promoters act to control expression of the foreign genes within root nodules formed by rhizobial bacterial strains in symbiotic combination with host plants.A rhizobium diagnostic segment (RDS) is also provided comprising a DNA segment found at more than one location in rhizobia, the RDS being species-specific, and detectable by DNA hybridization under stringent conditions. A recombinant plasmid comprising a RDS and a bacterial strain containing the plasmid are provided. Methods are provided for identifying species and strains of field isolates of Rhizobium, using RDS's. One RDS exemplified comprises 5' sequences from the R. trifolii nifH gene.

    摘要翻译: 本发明提供了来自三叶草nifH基因的有用的启动子,用于构建重组分子以调节外来基因以在期望的条件下表达。 特别地,启动子用于控制与宿主植物共生组合的由根瘤菌细菌菌株形成的根结节内的外源基因的表达。 还提供了根瘤菌诊断片段(RDS),其包含在根瘤菌中多于一个位置处发现的DNA片段,RDS是物种特异性的,并且可以在严格条件下通过DNA杂交检测。 提供了包含RDS和含有该质粒的细菌菌株的重组质粒。 提供了使用RDS来鉴定根瘤菌的分离株的种类和菌株的方法。 一个RDS示例包括来自三叶草nifH基因的5'序列。

    Nodulation gene promoter
    3.
    发明授权
    Nodulation gene promoter 失效
    结瘤基因启动子

    公开(公告)号:US5484718A

    公开(公告)日:1996-01-16

    申请号:US875300

    申请日:1986-06-17

    摘要: The invention includes a consensus nucleotide sequence found in the promoter regions of those Rhizobium and Bradyrhizobium nodulation genes which are activated by chemical inducer(s) in legume exudate. A promoter comprising the consensus sequence is a legume exudate-inducible promoter. A recombinant gene comprising a structural gene and a legume exudate-inducible promoter is selectively expressed in the presence of legume exudate or chemical inducer(s) and a functional nodD gene. The consensus nucleotide sequence isATCCAYNNYGYRGATGNWYKYKATCSAAWCAATCRATTTTACCARWYYKNSRR whereN is A, G, C or TY is C or TR is A or GW is A or TK is G or T andS is C or G.

    摘要翻译: 本发明包括在由豆科渗出物中的化学诱导剂活化的那些根瘤菌和Bradyrhizobium结瘤基因的启动子区域中发现的共有核苷酸序列。 包含共有序列的启动子是豆科渗出物诱导型启动子。 在豆类渗出物或化学诱导剂和功能性nodD基因的存在下,选择性地表达包含结构基因和豆科渗出物诱导型启动子的重组基因。 共有核苷酸序列为ATCCAYNNYGYRGATGNWYKYKATCSAAWCAATCRATTTTACCARWYYKNSRR,其中N为A,G,C或T Y为C或T R为A或G W为A或T K为G或T,S为C或G。

    Nodulation inducing factors
    5.
    发明授权
    Nodulation inducing factors 失效
    结瘤诱导因素

    公开(公告)号:US5141745A

    公开(公告)日:1992-08-25

    申请号:US393050

    申请日:1989-08-08

    摘要: Nodulation gene-inducing factors have been isolated and identified. A number of substituted flavones, some of which are components of exudates and extracts of leguminous plants, have been found to induce expression of certain nodulation genes in Rhizobium strains. Three components of clover extract 7,4'-dihydroxyflavone, 7,4'-dihydroxy-3'methoxyflavone, and 4'-hydroxy-7-methoxyflavone have been identified as nodulation inducers of certain Rhizobium trifolii nod genes. A number of other flavonoids including flavones, flavanones, flavanols and dihydroflavanols, not necessarily found in legume exudates, were also found to induce expression of certain nodulation genes.

    摘要翻译: 已经分离和鉴定了结瘤基因诱导因子。 已经发现许多取代的黄酮,其中一些是渗出物的组分和豆科植物的提取物,以诱导根瘤菌菌株中某些结瘤基因的表达。 三叶草提取物7,4'-二羟基黄酮,7,4'-二羟基-3'-甲氧基黄酮和4'-羟基-7-甲氧基黄酮的三个成分已被鉴定为某些根瘤菌三叶od基因的结瘤诱导物。 还发现许多其他类黄酮,包括黄豆素,黄烷醇,黄烷醇和二氢黄烷醇,不一定在豆科渗出物中发现,诱导某些结瘤基因的表达。