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公开(公告)号：US5023180A
公开(公告)日：1991-06-11
申请号：US61848
申请日：1987-06-11
IPC分类号： C07K14/195 , C12N15/74
CPC分类号： C07K14/195 , C12N15/743 , C07K2319/00 , C07K2319/61 , Y10S435/878
摘要： Nodulation regulatory genes (nodD genes) of Bradyrhizobium japonicum strains have been isolated and sequenced. Recombinant DNA molecules and vectors containing these regulatory genes are described. These genes, molecules and vectors are useful in the genetic engineering of Rhizobium and Bradyrhizobium strains. A method for selective expression of structural genes in response to the application of chemical factors which induce B. japonicum nod genes which employs these genes is described.
摘要翻译： 已经分离并测序了Bradyrhizobium japonicum菌株的结瘤调节基因（nodD基因）。 描述了重组DNA分子和含有这些调节基因的载体。 这些基因，分子和载体可用于根瘤菌和Bradyrhizobium菌株的遗传工程。 描述了应用这些诱导使用这些基因的日本粳稻节点基因的化学因子的应用来选择性表达结构基因的方法。
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公开(公告)号：US5001061A
公开(公告)日：1991-03-19
申请号：US109868
申请日：1987-10-16
申请人： Barry G. Rolfe , John Shine , Kieran F. Scott , Hauke Hennecke , Kieran F. Scott
发明人： Barry G. Rolfe , John Shine , Kieran F. Scott , Hauke Hennecke , Kieran F. Scott
IPC分类号： C07K14/325 , C07K14/825 , C12N9/02 , C12N15/74 , C12N15/90
CPC分类号： C12N9/0095 , C07K14/325 , C07K14/825 , C12N15/743 , C12N15/90 , C12Y118/06001
摘要： The nifD promoter regions of Bradyrhizobium japonicum and Bradyhizobium sp. (Parasponia) have been sequenced and found to be significantly homologous. Recombinant DNA molecules comprising foreign genes under the control of such promoters are provided. Rhizobial species containing such recombinant constructions, either in plasmids or integrated into the genome, are provided. These are especially useful for expressing desired foreign genes within root nodules.
摘要翻译： 日本Bradyrhizobium japonicum和Bradyhizobium sp。的nifD启动子区 （Parasponia）已被测序并发现是显着同源的。 提供了包含这种启动子控制下的外源基因的重组DNA分子。 提供了含有质粒或整合到基因组中的重组构建物的根瘤菌物种。 这些对于在根结节内表达所需的外来基因特别有用。
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3.发明授权L-phenylalanyl-tRNA synthetase mutants, a process for the preparation thereof and the use thereof for the in vivo incorporation of non-proteinogenous amino acids into peptides or proteins 失效L-苯丙氨酰-tRNA合成酶突变体，其制备方法及其用于将非蛋白质氨基酸体内掺入肽或蛋白质的用途
公开(公告)号：US5536652A
公开(公告)日：1996-07-16
申请号：US286262
申请日：1994-08-05
申请人： Hauke Hennecke , Peter Kast
发明人： Hauke Hennecke , Peter Kast
IPC分类号： A61K38/43 , C07K14/62 , C12N1/21 , C12N9/00 , C12N15/09 , C12R1/19 , C12N9/10 , C12N1/13 , C12N15/54 , C12N15/63
CPC分类号： C12N9/93 , C07K14/62 , C07K2319/00 , C07K2319/75
摘要： L-Phenylalanyl-tRNA synthetase mutants, a process for the preparation thereof and the use thereof for the in vivo incorporation of non-proteinogenous amino acids into peptides or proteins.The invention relates to L-phenylalanyl-tRNA synthetases from microorganisms which, by reason of a modification generated by genetic engineering, have an altered substrate selectivity, and to their preparation and use for the in vivo incorporation of non-proteinogenous amino acids into peptides or proteins.
摘要翻译： L-苯丙氨酰-tRNA合成酶突变体，其制备方法及其用于将非蛋白质性氨基酸体内掺入肽或蛋白质中的用途。 本发明涉及来自微生物的L-苯丙氨酰-tRNA合成酶，由于由遗传工程产生的修饰，底物选择性有所改变，以及它们的制备和用途将非蛋白质氨基酸体内掺入肽或 蛋白质。
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4.发明授权L-phenylalanyl-tRNA synthetase mutants, a process for the preparation thereof and the use thereof for the in vivo incorporation of non-proteinogenous amino acids into peptides or proteins 失效L-苯丙氨酰-tRNA合成酶突变体，其制备方法及其用于将非蛋白质氨基酸体内掺入肽或蛋白质的用途
公开(公告)号：US5370995A
公开(公告)日：1994-12-06
申请号：US822275
申请日：1992-01-21
申请人： Hauke Hennecke , Peter Kast
发明人： Hauke Hennecke , Peter Kast
IPC分类号： A61K38/43 , C07K14/62 , C12N1/21 , C12N9/00 , C12N15/09 , C12R1/19 , C12N9/10 , C12N15/12 , C12N15/54 , C12P21/00
CPC分类号： C12N9/93 , C07K14/62 , C07K2319/00 , C07K2319/75
摘要： L-Phenylalanyl-tRNA synthetase mutants, a process for the preparation thereof and the use thereof for the in vivo incorporation of non-proteinogenous amino acids into peptides or proteins.The invention relates to L-phenylalanyl-tRNA synthetases from microorganisms which, by reason of a modification generated by genetic engineering, have an altered substrate selectivity, and to their preparation and use for the in vivo incorporation of non-proteinogenous amino acids into peptides or proteins.
摘要翻译： L-苯丙氨酰-tRNA合成酶突变体，其制备方法及其用于将非蛋白质性氨基酸体内掺入肽或蛋白质中的用途。 本发明涉及来自微生物的L-苯丙氨酰-tRNA合成酶，由于由遗传工程产生的修饰，底物选择性有所改变，以及它们的制备和用途将非蛋白质氨基酸体内掺入肽或 蛋白质。
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公开(公告)号：US5059533A
公开(公告)日：1991-10-22
申请号：US219738
申请日：1988-07-15
IPC分类号： C07K14/79
CPC分类号： C07K14/79 , Y10S435/878
摘要： Rhizobial ferredoxin genes and proteins are provided. Rhizobial ferredoxins are useful to enhance the nitrogenase systems of rhizobia. Useful rhizobial ferredoxin diagnostic segments are also provided comprising DNA sequences encoding the characteristic ferredoxin cysteine residue patterns. Exemplied rhizobial ferredoxin genes are fixX of Rhizobium trifolii and Rhizobium meliloti Between fixC and nifA, fixY of Rhizobium meliloti down stream from nifB, and frxA of Bradyrhizobium japonicum.
摘要翻译： 提供了根瘤菌铁氧还蛋白基因和蛋白质。 根瘤菌铁氧还蛋白有助于增强根瘤菌的氮酶体系。 还提供了有用的根瘤菌铁氧还蛋白诊断片段，其包含编码特征性铁氧还蛋白半胱氨酸残基模式的DNA序列。 示例性根瘤菌铁氧还蛋白基因是根瘤菌和根瘤菌在FixC和nifA之间的固定物，来自nifB的下游根瘤菌的固定物，以及日本的Bradyrhizobium japonicum的frxA。
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公开(公告)号：US5008194A
公开(公告)日：1991-04-16
申请号：US109869
申请日：1987-10-16
申请人： Barry G. Rolfe , John Shine , Kieran F. Scott , Hauke Hennecke
发明人： Barry G. Rolfe , John Shine , Kieran F. Scott , Hauke Hennecke
IPC分类号： C07K14/325 , C07K14/825 , C12N9/02 , C12N15/00 , C12N15/74 , C12N15/90
CPC分类号： C12N9/0095 , C07K14/325 , C07K14/825 , C12N15/00 , C12N15/743 , C12N15/90 , C12Y118/06001
摘要： The nifH promoter regions of Bradyrhizobium japonicum and Bradyrhizobium sp. (parasponia) have been sequenced and found to be significantly homologous. Recombinant DNA molecules comprising foreign genes under the control of such promoters are provided. Rhizobial species containing such recombinant constructions, either in plasmids or integrated into the genome, are provided. These are especially useful for expressing desired foreign genes within root nodules.
摘要翻译： 日本Bradyrhizobium japonicum和Bradyrhizobium sp。的nifH启动子区 （parasponia）已被测序并发现是显着同源的。 提供了包含这种启动子控制下的外源基因的重组DNA分子。 提供了含有质粒或整合到基因组中的重组构建物的根瘤菌物种。 这些对于在根结节内表达所需的外来基因特别有用。
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