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    Fluorescent sensor compounds for detecting saccharides
    3.
    发明授权
    Fluorescent sensor compounds for detecting saccharides 失效
    用于检测糖类的荧光传感器化合物

    公开(公告)号：US06916660B2

    公开(公告)日：2005-07-12

    申请号：US10437362

    申请日：2003-05-13

    申请人： Binghe Wang ,  Brent Weston ,  Wenqian Yang

    发明人： Binghe Wang ,  Brent Weston ,  Wenqian Yang

    IPC分类号： A61K31/69 ,  C07F5/02 ,  G01N33/574 ,  G01N33/66 ,  G01N21/64

    CPC分类号： C07F5/025 ,  A61K31/69 ,  G01N33/57492 ,  G01N33/66 ,  Y10T436/143333 ,  Y10T436/144444

    摘要： Fluorescent sensor compounds having the formula: wherein L is selected from the group consisting of alkyl, alkylene, aryl, cycloalkyl, alkoxy, aryloxy, arylalkyl, and arylalkyloxyl; each m, m′, n, n′, p, and p′ is independently an integer from 0 to 4, inclusive; and each R1, R′1, R2, R′2, R3 and R′3 is independently selected from the group consisting of hydrogen, alkyl, alkylene, aryl, cycloalkyl, alkoxy, aryloxy, arylalkyl, arylalkyloxyl, halo, substituted and unsubstituted amino, and substituted and unsubstituted thiol, are useful for the selective detection of saccharides such as glucose and sialyl Lewis X. The compounds find particular use in detecting saccharides in biological samples, and in detecting cancer cells that express cell surface polysaccharides such as sialyl Lewis X.

    摘要翻译： 具有下式的荧光传感器化合物：其中L选自烷基，亚烷基，芳基，环烷基，烷氧基，芳氧基，芳基烷基和芳基烷氧基; 每个m，m'，n，n'，p和p'独立地为0至4的整数，包括0和4; 和每个R 1，R'1，R 2，R'2，R 3， R 3'和R 3'独立地选自氢，烷基，亚烷基，芳基，环烷基，烷氧基，芳氧基，芳基烷基，芳基烷氧基，卤素，取代和未取代的氨基， 未经取代的硫醇可用于选择性检测糖类如葡萄糖和唾液酸路易斯X.这些化合物特别用于检测生物样品中的糖类，以及检测表达细胞表面多糖的癌细胞，如唾液酸路易斯X.

    Nucleotides and aptamers containing boronic acid groups having biased binding to glycosylated proteins, and uses thereof
    7.
    发明授权
    Nucleotides and aptamers containing boronic acid groups having biased binding to glycosylated proteins, and uses thereof 有权
    含有与糖基化蛋白质有偏离结合的硼酸基团的核苷酸和适体及其用途

    公开(公告)号：US09096856B2

    公开(公告)日：2015-08-04

    申请号：US12669593

    申请日：2008-07-17

    申请人： Binghe Wang ,  Minyong Li ,  Zhen Huang ,  Na Lin

    发明人： Binghe Wang ,  Minyong Li ,  Zhen Huang ,  Na Lin

    IPC分类号： C12Q1/68 ,  C12N15/115 ,  C07H19/00 ,  C07H23/00 ,  C40B10/00 ,  G01N33/68 ,  C07H19/04 ,  C07H21/00 ,  C12N15/10 ,  G01N33/86

    CPC分类号： C12N15/115 ,  C07H19/04 ,  C07H21/00 ,  C07H23/00 ,  C12N15/1048 ,  C12N2310/16 ,  C12N2310/33 ,  G01N33/68 ,  G01N33/6893 ,  G01N33/86

    摘要： The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein. Other aspects of the disclosure encompass methods for the use of the modified aptamers to detect glycosylated species of a polypeptide or protein.

    摘要翻译： 本公开内容包括选择性结合靶糖基化多肽或蛋白质并且通过与核苷酸的核苷碱基连接的硼酸对聚糖具有偏向亲和力的寡核苷酸适体。 本公开还包括用于分离选择性结合靶糖基化多肽的适体的方法，其中从具有至少一个具有与碱基连接的硼酸标记的至少一个核苷酸的随机寡核苷酸群体中选择第一适配子亚群 与目标糖基化多肽或蛋白质结合。 然后在不使用硼酸修饰的TTP的情况下扩增该亚群，并且选择不与靶糖基化多肽或蛋白质结合的扩增产物。 然后使用硼酸修饰的TTP扩增适配体的第二亚群，以提供能够选择性结合靶多肽或蛋白质的糖基化位点的硼酸修饰的适配体群。 本公开的其它方面包括使用修饰的适体检测多肽或蛋白质的糖基化物种的方法。

    APTAMER INHIBITION OF THROMBUS FORMATION
    8.
    发明申请
    APTAMER INHIBITION OF THROMBUS FORMATION 审中-公开
    APTAMER抑制血栓形成

    公开(公告)号：US20110144187A1

    公开(公告)日：2011-06-16

    申请号：US13058627

    申请日：2009-08-14

    申请人： Binghe Wang ,  Minyong Li ,  Nanting Ni ,  Yidan Liu

    发明人： Binghe Wang ,  Minyong Li ,  Nanting Ni ,  Yidan Liu

    IPC分类号： A61K48/00 ,  C07H21/00 ,  C12N15/63 ,  A61K31/7088 ,  A61P7/02

    CPC分类号： C12N15/115 ,  A61K31/7088 ,  C12N2310/16

    摘要： Boronic acid-modified DNA-based aptamers can be selected to recognize fibrinogen through binding at a glycosylation site and thus are useful for probing the effect of glycosylation pattern changes on the ability for fibrinogen to mediate blood coagulation. In addition, the aptamers of the disclosure also have anticoagulation effects due to their binding to fibrinogen and its cleavage product fibrin. The present disclosure, therefore, encompasses methods for inhibiting fibrin coagulation with an aptamer capable of specifically binding to a glycosylation site of fibrinogen or fibrin. The disclosure further provides oligonucleotide aptamers comprising at least one nucleotide having a boronic acid thereon, where the aptamer is capable of selectively binding to a glycosylation site of fibrinogen, or the derivative thereof.

    摘要翻译： 可以通过在糖基化位点结合来选择硼酸修饰的基于DNA的适体来识别纤维蛋白原，因此可用于探测糖基化模式变化对纤维蛋白原介导血液凝固的能力的影响。 此外，本公开的适体也由于其与纤维蛋白原及其裂解产物纤维蛋白的结合而具有抗凝作用。 因此，本公开内容包括用能够特异性结合纤维蛋白原或纤维蛋白的糖基化位点的适体抑制纤维蛋白凝结的方法。 本公开还提供了包含至少一个其上具有硼酸的核苷酸的寡核苷酸适体，其中适体能够选择性地结合纤维蛋白原的糖基化位点或其衍生物。