利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

18 条记录 (耗时 0.024 秒)

    Nucleotides and aptamers containing boronic acid groups having biased binding to glycosylated proteins, and uses thereof
    1.
    发明授权
    Nucleotides and aptamers containing boronic acid groups having biased binding to glycosylated proteins, and uses thereof 有权
    含有与糖基化蛋白质有偏离结合的硼酸基团的核苷酸和适体及其用途

    公开(公告)号：US09096856B2

    公开(公告)日：2015-08-04

    申请号：US12669593

    申请日：2008-07-17

    申请人： Binghe Wang ,  Minyong Li ,  Zhen Huang ,  Na Lin

    发明人： Binghe Wang ,  Minyong Li ,  Zhen Huang ,  Na Lin

    IPC分类号： C12Q1/68 ,  C12N15/115 ,  C07H19/00 ,  C07H23/00 ,  C40B10/00 ,  G01N33/68 ,  C07H19/04 ,  C07H21/00 ,  C12N15/10 ,  G01N33/86

    CPC分类号： C12N15/115 ,  C07H19/04 ,  C07H21/00 ,  C07H23/00 ,  C12N15/1048 ,  C12N2310/16 ,  C12N2310/33 ,  G01N33/68 ,  G01N33/6893 ,  G01N33/86

    摘要： The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein. Other aspects of the disclosure encompass methods for the use of the modified aptamers to detect glycosylated species of a polypeptide or protein.

    摘要翻译： 本公开内容包括选择性结合靶糖基化多肽或蛋白质并且通过与核苷酸的核苷碱基连接的硼酸对聚糖具有偏向亲和力的寡核苷酸适体。 本公开还包括用于分离选择性结合靶糖基化多肽的适体的方法，其中从具有至少一个具有与碱基连接的硼酸标记的至少一个核苷酸的随机寡核苷酸群体中选择第一适配子亚群 与目标糖基化多肽或蛋白质结合。 然后在不使用硼酸修饰的TTP的情况下扩增该亚群，并且选择不与靶糖基化多肽或蛋白质结合的扩增产物。 然后使用硼酸修饰的TTP扩增适配体的第二亚群，以提供能够选择性结合靶多肽或蛋白质的糖基化位点的硼酸修饰的适配体群。 本公开的其它方面包括使用修饰的适体检测多肽或蛋白质的糖基化物种的方法。

    NUCLEOTIDES AND APTAMERS CONTAINING BORONIC ACID GROUPS HAVING BIASED BINDING TO GLYCOSYLATED PROTEINS, AND USES THEREOF
    2.
    发明申请
    NUCLEOTIDES AND APTAMERS CONTAINING BORONIC ACID GROUPS HAVING BIASED BINDING TO GLYCOSYLATED PROTEINS, AND USES THEREOF 有权
    含有具有粘合蛋白质的蛋白质的硼酸组合的核苷酸和APTAMER及其用途

    公开(公告)号：US20130184160A1

    公开(公告)日：2013-07-18

    申请号：US12669593

    申请日：2008-07-17

    申请人： Binghe Wang ,  Minyong Li ,  Zhen Huang ,  Na Lin

    发明人： Binghe Wang ,  Minyong Li ,  Zhen Huang ,  Na Lin

    IPC分类号： C12N15/115 ,  C07H23/00 ,  G01N33/86 ,  C12N15/10 ,  G01N33/68

    CPC分类号： C12N15/115 ,  C07H19/04 ,  C07H21/00 ,  C07H23/00 ,  C12N15/1048 ,  C12N2310/16 ,  C12N2310/33 ,  G01N33/68 ,  G01N33/6893 ,  G01N33/86

    摘要： The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein. Other aspects of the disclosure encompass methods for the use of the modified aptamers to detect glycosylated species of a polypeptide or protein.

    摘要翻译： 本公开内容包括选择性结合靶糖基化多肽或蛋白质并且通过与核苷酸的核苷碱基连接的硼酸对聚糖具有偏向亲和力的寡核苷酸适体。 本公开还包括用于分离选择性结合靶糖基化多肽的适体的方法，其中从具有至少一个具有与碱基连接的硼酸标记的至少一个核苷酸的随机寡核苷酸群体中选择第一适配子亚群 与目标糖基化多肽或蛋白质结合。 然后在不使用硼酸修饰的TTP的情况下扩增该亚群，并且选择不与靶糖基化多肽或蛋白质结合的扩增产物。 然后使用硼酸修饰的TTP扩增适配体的第二亚群，以提供能够选择性结合靶多肽或蛋白质的糖基化位点的硼酸修饰的适配体群。 本公开的其它方面包括使用修饰的适体检测多肽或蛋白质的糖基化物种的方法。

    APTAMER INHIBITION OF THROMBUS FORMATION
    3.
    发明申请
    APTAMER INHIBITION OF THROMBUS FORMATION 审中-公开
    APTAMER抑制血栓形成

    公开(公告)号：US20110144187A1

    公开(公告)日：2011-06-16

    申请号：US13058627

    申请日：2009-08-14

    申请人： Binghe Wang ,  Minyong Li ,  Nanting Ni ,  Yidan Liu

    发明人： Binghe Wang ,  Minyong Li ,  Nanting Ni ,  Yidan Liu

    IPC分类号： A61K48/00 ,  C07H21/00 ,  C12N15/63 ,  A61K31/7088 ,  A61P7/02

    CPC分类号： C12N15/115 ,  A61K31/7088 ,  C12N2310/16

    摘要： Boronic acid-modified DNA-based aptamers can be selected to recognize fibrinogen through binding at a glycosylation site and thus are useful for probing the effect of glycosylation pattern changes on the ability for fibrinogen to mediate blood coagulation. In addition, the aptamers of the disclosure also have anticoagulation effects due to their binding to fibrinogen and its cleavage product fibrin. The present disclosure, therefore, encompasses methods for inhibiting fibrin coagulation with an aptamer capable of specifically binding to a glycosylation site of fibrinogen or fibrin. The disclosure further provides oligonucleotide aptamers comprising at least one nucleotide having a boronic acid thereon, where the aptamer is capable of selectively binding to a glycosylation site of fibrinogen, or the derivative thereof.

    摘要翻译： 可以通过在糖基化位点结合来选择硼酸修饰的基于DNA的适体来识别纤维蛋白原，因此可用于探测糖基化模式变化对纤维蛋白原介导血液凝固的能力的影响。 此外，本公开的适体也由于其与纤维蛋白原及其裂解产物纤维蛋白的结合而具有抗凝作用。 因此，本公开内容包括用能够特异性结合纤维蛋白原或纤维蛋白的糖基化位点的适体抑制纤维蛋白凝结的方法。 本公开还提供了包含至少一个其上具有硼酸的核苷酸的寡核苷酸适体，其中适体能够选择性地结合纤维蛋白原的糖基化位点或其衍生物。

    Compositions for regulating or modulating quorum sensing in bacteria, methods of using the compounds, and methods of regulating or modulating quorum sensing in bacteria
    5.
    发明授权
    Compositions for regulating or modulating quorum sensing in bacteria, methods of using the compounds, and methods of regulating or modulating quorum sensing in bacteria 有权
    用于调节或调节细菌中群体感应的组合物，使用该化合物的方法，以及调节或调节细菌中群体感应的方法

    公开(公告)号：US08653258B2

    公开(公告)日：2014-02-18

    申请号：US12597825

    申请日：2008-06-06

    申请人： Binghe Wang ,  Nanting Ni ,  Junfeng Wang ,  Chung-Dar Lu ,  Han-Ting Chou ,  Minyong Li ,  Shilong Zheng ,  Yunfeng Cheng ,  Hanjing Peng

    发明人： Binghe Wang ,  Nanting Ni ,  Junfeng Wang ,  Chung-Dar Lu ,  Han-Ting Chou ,  Minyong Li ,  Shilong Zheng ,  Yunfeng Cheng ,  Hanjing Peng

    IPC分类号： C07F5/02 ,  C12Q1/02

    CPC分类号： C07D333/34 ,  C07F5/025

    摘要： The present disclosure encompasses compounds and compositions that are useful as specific AI-2 antagonists for the control of bacterial quorum sensing. Although the AI-2 antagonists according to the present disclosure may not have bactericidal effect, their ability to attenuate virulence, drug resistance, and/or biofilm formation have therapeutic benefits. In addition, the AI-2 antagonists of the present disclosure can also be used as tools to probe bacterial AI-2 functions. The present disclosure also encompasses methods for inhibiting or attenuating microbial virulence, biofilm formation, and drug resistance. The methods are suitable for preventing bacteria from accruing and forming extensive biofilms that may be a health or hygiene hazard or a physical issue, such as in the blockage of water or fuel lines.

    摘要翻译： 本公开涵盖可用作用于控制细菌群体感测的特异性AI-2拮抗剂的化合物和组合物。 虽然根据本公开的AI-2拮抗剂可能不具有杀菌作用，但是它们减轻毒力，耐药性和/或生物膜形成的能力具有治疗益处。 此外，本公开的AI-2拮抗剂也可以用作探针细菌AI-2功能的工具。 本公开还包括抑制或减弱微生物毒力，生物膜形成和耐药性的方法。 该方法适用于防止细菌累积和形成广泛的生物膜，这些生物膜可能是健康或卫生危害或物理问题，例如堵塞水或燃料管线。

    Chemosensors for hydrogen sulfide
    10.
    发明授权
    Chemosensors for hydrogen sulfide 有权
    化学传感器用于硫化氢

    公开(公告)号：US09175165B2

    公开(公告)日：2015-11-03

    申请号：US13997963

    申请日：2012-01-06

    申请人： Hanjing Peng ,  Binghe Wang

    发明人： Hanjing Peng ,  Binghe Wang

    IPC分类号： G01N33/00 ,  G01N31/02 ,  G01N31/00 ,  G01N21/33 ,  C09B56/20 ,  G01N21/64 ,  G01N31/22

    CPC分类号： C09B56/20 ,  G01N21/64 ,  G01N21/643 ,  G01N31/22 ,  G01N33/0044 ,  Y10T436/184

    摘要： Selective chemosensing agents for hydrogen sulfide are provided. The chemosensing agents can act fast under mild conditions, are chemically stable for long-term storage, are sensitive for detection under near physiological conditions, show a linear concentration-signal relationship within physiologically relevant hydrogen sulfide concentration ranges for easy quantitation, show minimal or no interference by other anions in the blood, and are functional in aqueous solutions and blood plasma.

    摘要翻译： 提供硫化氢的选择性化学感应剂。 化学感应剂可在温和条件下快速作用，对于长期储存具有化学稳定性，对近生理条件下的检测敏感，在生理相关硫化氢浓度范围内显示线性浓度 - 信号关系，易于定量，显示最小或无 其他阴离子在血液中的干扰，并且在水溶液和血浆中起作用。