公开(公告)号：US20150301033A1

公开(公告)日：2015-10-22

申请号：US14689791

申请日：2015-04-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun GUO ,  Young Joon Kim

IPC: G01N33/543 ,  C12Q1/68 ,  B01L7/00 ,  B01L3/00

CPC classification number: G01N33/54366 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2300/043 ,  B01L2300/047 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q2565/629 ,  C12Q2600/156

Abstract: Provided herein are cartridges, devices, and methods for carrying out multistep assays on a microfluidic scale. A cartridge includes a block frame comprising a well, wherein the well comprises an outlet, at least one inlet, and a bottom surface; a plurality of containers, wherein each container is connected to the well via a microchannel leading to the at least one inlet; and an openable cover, which cover when closed is configured to enclose an assay surface and form a gap between (i) the assay surface and the bottom surface of the well or (ii) the assay surface and the cover. The gap can be formed by a spacer that extends from the bottom surface of the well or from the outer edge of the cover. Methods include flowing liquid into the gap and/or through an opening adjacent to the assay surface.

Abstract translation: 本文提供了用于在微流控标尺上进行多步测定的盒，装置和方法。 盒包括包括井的块框架，其中井包括出口，至少一个入口和底表面; 多个容器，其中每个容器经由通向所述至少一个入口的微通道连接到所述孔; 和可打开的盖，其在关闭时被覆盖以配置以包围测定表面并在（i）测定表面和孔的底部表面之间形成间隙，或（ii）测定表面和盖。 该间隙可以由从井底表面或从盖的外边缘延伸的间隔件形成。 方法包括将液体流入间隙和/或通过与测定表面相邻的开口。

公开(公告)号：US20150093743A1

公开(公告)日：2015-04-02

申请号：US14479132

申请日：2014-09-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amir M. SADRI ,  Kun GUO ,  Daniel Y. CHU ,  Nenad KIRCANSKI ,  Paul J. PATT ,  Tal ROSENZWEIG

IPC: G01N15/14 ,  B01L3/00 ,  C12Q1/24

CPC classification number: G01N15/1459 ,  B01L3/0265 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/084 ,  C12Q1/24 ,  G01N15/1404 ,  G01N2015/1413 ,  G01N2015/149 ,  Y10T436/25 ,  Y10T436/25375

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

Abstract translation: 用于微流体操纵，分配和/或分选颗粒例如细胞和/或珠粒的系统，包括装置和方法。