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    Processing of amplified DNA fragments for sequencing
    5.
    发明授权
    Processing of amplified DNA fragments for sequencing 有权
    加工扩增的DNA片段进行测序

    公开(公告)号：US09506110B2

    公开(公告)日：2016-11-29

    申请号：US13515953

    申请日：2010-12-15

    申请人： Masafumi Muratani ,  Huck Hui Ng

    发明人： Masafumi Muratani ,  Huck Hui Ng

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6846 ,  C12Q2525/179 ,  C12Q2525/131 ,  C12Q2521/313

    摘要： A processing method to trim ends of DNA fragments, exposing the internal DNA part to give original DNA sequence information enabling application of next generation sequencing for DNA samples to be amplified by DOP-PCR or other primer dependent amplification methods. Specifically, nucleic acids are amplified using primers comprising a recognition site for a restriction enzyme, for example Bpml or Mmel. Primer sequences are removed by cleavage with the restriction enzyme.

    摘要翻译： 缩短DNA片段末端的处理方法，暴露内部DNA部分，得到原始DNA序列信息，使得可以通过DOP-PCR或其他引物依赖性扩增方法扩增DNA样品的下一代测序。 具体地，使用包含限制酶的识别位点的引物（例如Bpml或Mmel）扩增核酸。 引物序列通过用限制酶切割除去。

    PROCESSING OF AMPLIFIED DNA FRAGMENTS FOR SEQUENCING
    7.
    发明申请
    PROCESSING OF AMPLIFIED DNA FRAGMENTS FOR SEQUENCING 有权
    用于测序的扩增DNA片段的处理

    公开(公告)号：US20120252702A1

    公开(公告)日：2012-10-04

    申请号：US13515953

    申请日：2010-12-15

    申请人： Masafumi Muratani ,  Huck Hui Ng

    发明人： Masafumi Muratani ,  Huck Hui Ng

    IPC分类号： C12P19/34 ,  C07H21/04 ,  C12Q1/68 ,  C40B50/06

    CPC分类号： C12Q1/6846 ,  C12Q2525/179 ,  C12Q2525/131 ,  C12Q2521/313

    摘要： A processing method to trim ends of DNA fragments, exposing the internal DNA part to give original DNA sequence information enabling application of next generation sequencing for DNA samples to be amplified by DOP-PCR or other primer dependent amplification methods. Specifically, nucleic acids are amplified using primers comprising a recognition site for a restriction enzyme, for example Bpml or Mmel. Primer sequences are removed by cleavage with the restriction enzyme.

    摘要翻译： 缩短DNA片段末端的处理方法，暴露内部DNA部分，得到原始DNA序列信息，使得可以通过DOP-PCR或其他引物依赖性扩增方法扩增DNA样品的下一代测序。 具体地，使用包含限制酶的识别位点的引物（例如Bpml或Mmel）扩增核酸。 引物序列通过用限制酶切割除去。

    NUCLEAR RECEPTOR AND MUTANT THEREOF AND THE USE OF THE SAME IN THE REPROGRAMMING OF CELLS
    8.
    发明申请
    NUCLEAR RECEPTOR AND MUTANT THEREOF AND THE USE OF THE SAME IN THE REPROGRAMMING OF CELLS 有权
    核受体及其突变体及其在细胞重编中的应用

    公开(公告)号：US20120189596A1

    公开(公告)日：2012-07-26

    申请号：US13499607

    申请日：2010-09-30

    申请人： Dominic Jian-Chien Heng ,  Huck Hui Ng

    发明人： Dominic Jian-Chien Heng ,  Huck Hui Ng

    IPC分类号： A61K35/12 ,  C12N5/071 ,  C12N15/85

    CPC分类号： C12N5/0696 ,  C12N15/85 ,  C12N2501/38 ,  C12N2501/602 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00

    摘要： According to the invention there is provided methods for inducing pluripotent stem cells in vitro, comprising introducing a gene or polypeptide of a nuclear receptor and one or more gene or polypeptide selected from the group consisting of Sox, Krüppel-like factor or the myc family, to cells in vitro. The present invention also provides vectors and compositions for producing the same and methods for using the induced pluripotent stem cell for treating a patient in need of a pluripotent stem cell treatment.

    摘要翻译： 根据本发明，提供了在体外诱导多能干细胞的方法，包括引入核受体的基因或多肽和选自Sox，Krüppel样因子或myc家族的一种或多种基因或多肽， 到体外细胞。 本发明还提供了用于制备其的载体和组合物以及使用诱导多能干细胞治疗需要多能干细胞治疗的患者的方法。

    Method for maintaining pluripotency of stem/progenitor cells
    9.
    发明申请
    Method for maintaining pluripotency of stem/progenitor cells 审中-公开
    保持干/祖细胞多能性的方法

    公开(公告)号：US20090018059A1

    公开(公告)日：2009-01-15

    申请号：US11661957

    申请日：2005-09-02

    申请人： Paul Robson ,  David Rodda ,  Huck Hui Ng

    发明人： Paul Robson ,  David Rodda ,  Huck Hui Ng

    IPC分类号： A61K38/17 ,  C12N5/08 ,  C12Q1/02 ,  A61P35/00 ,  C12Q1/68 ,  C40B30/06

    CPC分类号： C12N5/0606 ,  C12N15/113 ,  C12N2310/13 ,  C12N2310/14 ,  C12N2501/60 ,  C12N2510/00

    摘要： The present invention relates to a method for maintaining pluripotency and/or self-renewing characteristics of stem/progenitor cells. The invention also relates to a method for modulating gene expression in a cell. The methods include contacting at least two transcription factors, or a functional fragment thereof, with the promoter region of the nanog gene. One of the at least two transcription factors is selected from the POU- and homeo-domain-containing transcription factors. Another of the at least two transcription factors is selected from the HMG domain-containing transcription factors. The method further comprises allowing the at least two transcription factors to form a complex with a specific binding element within the nanog promoter. The complex thus formed regulates nanog gene expression by mediating transcriptional activation.

    摘要翻译： 本发明涉及一种用于保持干细胞/祖细胞的多能性和/或自我更新特性的方法。 本发明还涉及调节细胞中基因表达的方法。 所述方法包括将至少两个转录因子或其功能片段与纳米基因的启动子区域接触。 至少两种转录因子中的一种选自含POU和同源结构域的转录因子。 至少两种转录因子中的另一种选自含HMG结构域的转录因子。 该方法还包括允许至少两个转录因子与纳米启动子内的特异性结合元件形成复合物。 由此形成的复合物通过介导转录激活调节纳米基因表达。