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    Method for Specific Covalent Coupling of Antibody Using a Photoactivable Protein G Variant
    1.
    发明申请
    Method for Specific Covalent Coupling of Antibody Using a Photoactivable Protein G Variant 审中-公开
    使用可光化蛋白G变体的抗体特异性共价偶联的方法

    公开(公告)号：US20110098197A1

    公开(公告)日：2011-04-28

    申请号：US12988722

    申请日：2008-05-27

    申请人： Bong Hyun Chung ,  Yong Won Jung ,  Jeong Min Lee

    发明人： Bong Hyun Chung ,  Yong Won Jung ,  Jeong Min Lee

    IPC分类号： C40B30/04 ,  C07K19/00 ,  C12N9/96 ,  C07K1/00 ,  C40B40/10 ,  C40B50/00

    CPC分类号： C07K14/315 ,  G01N33/531 ,  G01N33/533

    摘要： The present invention relates to a protein G variant comprising a mutated Fc binding domain, which is prepared by substituting cysteine for specific residues of the Fc-binding domain of protein G, and a method for preparing the same. Further, the present invention relates to a protein G variant comprising a cysteine mutated Fc binding domain that is site-selectively modified with a UV cross-linker. Further, the present invention relates to a method for UV cross-linking the protein G variant with antibody. The present invention relates to a protein G variant-antibody conjugate that is prepared by the above method. Further, the present invention provides a method for screening or analyzing antigens using the conjugate. Furthermore, the present invention provides a biochip or biosensor fabricated by linking the protein G variant to the surface of a solid support, and a method for fabricating the same. In addition, the present invention provides a method for immobilizing antibodies and analyzing antigens using the biochip or biosensor.

    摘要翻译： 本发明涉及包含通过用半胱氨酸代替蛋白G的Fc结合结构域的特异性残基制备的突变Fc结合结构域的蛋白G变体及其制备方法。 此外，本发明涉及包含用UV交联剂位点选择性修饰的半胱氨酸突变的Fc结合结构域的蛋白G变体。 此外，本发明涉及一种用抗体将蛋白G变体UV交联的方法。 本发明涉及通过上述方法制备的蛋白G变体 - 抗体缀合物。 此外，本发明提供了使用该缀合物筛选或分析抗原的方法。 此外，本发明提供了通过将蛋白G变体与固体支持物的表面连接而制造的生物芯片或生物传感器及其制造方法。 此外，本发明提供了使用生物芯片或生物传感器固定抗体和分析抗原的方法。

    Protein G-Oligonucleotide Conjugate
    2.
    发明申请
    Protein G-Oligonucleotide Conjugate 审中-公开
    蛋白G-寡核苷酸缀合物

    公开(公告)号：US20100203653A1

    公开(公告)日：2010-08-12

    申请号：US12665397

    申请日：2008-05-16

    申请人： Bong Hyun Chung ,  Yong Won Jung ,  Jeong Min Lee

    发明人： Bong Hyun Chung ,  Yong Won Jung ,  Jeong Min Lee

    IPC分类号： G01N33/543 ,  C07K14/00

    CPC分类号： C07K19/00 ,  C07K14/315 ,  G01N33/54393

    摘要： The present invention relates to a protein G conjugate, which is prepared by linking an N-terminal cysteine-tagged protein G variant with an oligonucleotide via a linker. The conjugate binds in a directional manner on the surface of a biochip and biosensor, thereby providing a biochip and biosensor having improved antibody immobilization ability.

    摘要翻译： 本发明涉及蛋白G缀合物，其通过将N-末端半胱氨酸标记的蛋白G变体与寡核苷酸通过接头连接来制备。 缀合物以有向的方式结合在生物芯片和生物传感器的表面上，从而提供具有改进的抗体固定能力的生物芯片和生物传感器。

    METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID
    3.
    发明申请
    METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID 有权
    使用肽混合物制备具有控制方向的抗体单体的方法

    公开(公告)号：US20100209945A1

    公开(公告)日：2010-08-19

    申请号：US12679982

    申请日：2007-10-18

    申请人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

    发明人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

    IPC分类号： G01N33/53 ,  C07K7/08 ,  C07K7/06 ,  G01N1/31

    CPC分类号： C07K17/00 ,  C07K7/02 ,  C07K7/08 ,  G01N33/54353 ,  G01N33/54393

    摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

    摘要翻译： 本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说是用于蛋白质固定的肽杂合物，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性 蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。 用于本发明的蛋白质固定的肽杂合物有助于控制抗体在各种固体表面上的取向，并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。 因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

    Method for preparing antibody monolayers which have controlled orientation using peptide hybrid
    5.
    发明授权
    Method for preparing antibody monolayers which have controlled orientation using peptide hybrid 有权
    使用肽杂交制备具有受控取向的抗体单层的方法

    公开(公告)号：US08236509B2

    公开(公告)日：2012-08-07

    申请号：US12679982

    申请日：2007-10-18

    申请人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

    发明人： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

    IPC分类号： G01N33/53

    CPC分类号： C07K17/00 ,  C07K7/02 ,  C07K7/08 ,  G01N33/54353 ,  G01N33/54393

    摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

    摘要翻译： 本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说，用于蛋白质固定的肽杂交体，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性 蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。 用于本发明的蛋白质固定化的肽杂合物有助于控制抗体在各种固体表面上的取向并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。 因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

    Electrode device for high frequency thermotherapy
    8.
    发明授权
    Electrode device for high frequency thermotherapy 有权
    高频热疗用电极装置

    公开(公告)号：US07354437B2

    公开(公告)日：2008-04-08

    申请号：US10546050

    申请日：2004-02-18

    申请人： Kyong Min Shin ,  Hyo Keun Lim ,  Hyun Chul Rhim ,  Jeong Min Lee

    发明人： Kyong Min Shin ,  Hyo Keun Lim ,  Hyun Chul Rhim ,  Jeong Min Lee

    IPC分类号： A61B18/18

    CPC分类号： A61N1/06 ,  A61B18/1477 ,  A61B18/1815 ,  A61B2018/00011 ,  A61B2018/00023 ,  A61B2018/00577 ,  A61B2018/1475 ,  A61B2218/002

    摘要： The object of the present invention is to provide an electrode device for high frequency thermotherapy, including a main casing, an electrode needle, a first engaging part, and a guide unit coupled to the engaging part to be longitudinally moved relative to the electrode needle. The guide unit has a guide tube to receive therein the electrode needle to expose a tip of the electrode needle to the outside of an end of the guide tube. Thus, the exposed length of the tip of the electrode needle is varied according to a position that the guide unit is coupled to the engaging part. The electrode device may further include a three-way valve -provided between the main casing and the guide unit to supply a liquid medicine or water t a desired part of a patient body.

    摘要翻译： 本发明的目的是提供一种用于高频热疗的电极装置，包括主壳体，电极针，第一接合部分和与相对于电极针纵向移动的接合部分的引导单元。 引导单元具有导管，用于在其中容纳电极针以将电极针的尖端暴露于引导管的端部的外侧。 因此，电极针的尖端的暴露长度根据引导单元联接到接合部的位置而变化。 电极装置还可以包括在主壳体和引导单元之间提供的三通阀，以向患者身体的期望部分供应液体药物或水。

    Cap assembly having storage chamber for secondary material with movable working member
    9.
    发明授权
    Cap assembly having storage chamber for secondary material with movable working member 有权
    盖组件具有用于具有可移动工作构件的辅助材料的储存室

    公开(公告)号：US08328010B2

    公开(公告)日：2012-12-11

    申请号：US12593681

    申请日：2007-03-27

    申请人： Jeong Min Lee ,  Seong Jae Lee

    发明人： Jeong Min Lee ,  Seong Jae Lee

    IPC分类号： B65D85/08

    CPC分类号： B65D51/2864 ,  B65D47/243

    摘要： The storage chamber for secondary material applicable to the discharging direction of contents stored in the container consists of body with storage space and inseparable operating section mounted around the globe. If above operating section moves up, opening and closing hole made or formed in the storage space of storage is opened and contents stored in the storage space is dropped and mixed with contents stored in the container. The mixed contents are discharged via a vent mounted on the top of operating section. A vent is not opened until the operating section is opened, and closing section of opening and closing hole is provided so that the opening and closing hole may not be not clogged after being opened.

    摘要翻译： 适用于储存在容器中的内容物的排放方向的二次材料储存室由具有储存空间的主体和安装在地球上的不可分离的操作部分组成。 如果上述操作部分向上移动，则打开储存空间中形成或形成的打开和关闭孔，并将存储在存储空间中的内容物与存储在容器中的内容物混合。 混合内容物通过安装在操作部分顶部的通风口排出。 在操作部分打开之前，排气口不会打开，并且设置打开和关闭孔的关闭部分，使得开启和关闭孔在打开之后不会被堵塞。

    APPARATUS AND METHOD FOR CONTROLLING THE TEMPERATURE OF A BLACK BODY
    10.
    发明申请
    APPARATUS AND METHOD FOR CONTROLLING THE TEMPERATURE OF A BLACK BODY 审中-公开
    用于控制黑体温度的装置和方法

    公开(公告)号：US20120073308A1

    公开(公告)日：2012-03-29

    申请号：US13375750

    申请日：2010-11-05

    申请人： Geon Hee Kim ,  Jeong Min Lee ,  Sun Choel Yang ,  Ki Soo Chang ,  Hyo Sik Kim ,  Moon Seop Yang

    发明人： Geon Hee Kim ,  Jeong Min Lee ,  Sun Choel Yang ,  Ki Soo Chang ,  Hyo Sik Kim ,  Moon Seop Yang

    IPC分类号： F25B21/02

    CPC分类号： G01J5/522 ,  G05D23/1919 ,  G05D23/27

    摘要： Provided is an apparatus for controlling temperature of a black body. The apparatus includes a black body emitting radiant energy of infrared rays; a first Peltier element adjacent to the black body, the first Peltier element having a 1a surface and a 1b surface that selectively emit or absorb heat; a copper plate adjacent to the 1b surface; a second Peltier element adjacent to the 2b surface, the second Peltier element having a 2a surface and a 2b surface that selectively emit or absorb heat; a heat transfer member comprising a bent surface adjacent to the 2a surface, and an arm extending toward both ends of the black body to be adjacent to the both ends of the black body and a control unit controlling the 2a surface to emit heat when the 1a surface emits heat, and the 2a surface to absorb heat when the 1a surface absorbs heat.

    摘要翻译： 提供一种用于控制黑体温度的装置。 该装置包括发射红外线辐射能的黑体; 与黑体相邻的第一珀耳帖元件，第一珀尔帖元件具有1a表面和1b表面，其选择性地发射或吸收热量; 邻近1b表面的铜板; 邻近2b表面的第二珀耳帖元件，第二珀耳帖元件具有2a表面和2b表面，其选择性地发射或吸收热量; 包括与2a表面相邻的弯曲表面的传热构件和朝向黑体的两端延伸以与黑体两端相邻的臂;以及控制单元，其控制2a表面在1a 表面发热，而2a表面在1a表面吸热时吸收热量。