公开(公告)号：US20210047740A1

公开(公告)日：2021-02-18

申请号：US16987173

申请日：2020-08-06

申请人： Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： C23F1/18 ,  H01J37/20 ,  C01B32/194 ,  C23F1/08

摘要： This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.

公开(公告)号：US20120043208A1

公开(公告)日：2012-02-23

申请号：US13176704

申请日：2011-07-05

申请人： Jian Jin ,  Mark D. Biggin ,  Robert A. Nordmeyer ,  Ming Dong ,  Earl W. Cornell ,  Megan Choi ,  Halina Ewa Witkowski ,  Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Jian Jin ,  Mark D. Biggin ,  Robert A. Nordmeyer ,  Ming Dong ,  Earl W. Cornell ,  Megan Choi ,  Halina Ewa Witkowski ,  Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： G01N33/559 ,  C25B7/00 ,  G01N27/447

CPC分类号： G01N27/44756 ,  G01N27/44769

摘要： A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.

摘要翻译： 一种用于有效收集通过凝胶电泳分离的分子的多通道凝胶电泳装置，因此可以进一步分析，鉴定或用作试剂或药物。 该装置使用将内源性复合物的多维分离与其组成的质谱监测相结合的新型“无标记”策略。 在该方法中，基于通过多个正交分离步骤的多肽集合的共同迁移来鉴定推定的蛋白质复合物。 显示大部分大肠杆菌蛋白质通过三个色谱步骤在粗提取物分馏期间保持在稳定的络合物中。

公开(公告)号：US11708637B2

公开(公告)日：2023-07-25

申请号：US16987173

申请日：2020-08-06

申请人： Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： C23F1/18 ,  H01J37/20 ,  C01B32/194 ,  C23F1/08 ,  A61P35/00 ,  A61K9/19 ,  A61K9/51 ,  C07H19/10

CPC分类号： C23F1/18 ,  A61K9/19 ,  A61K9/5169 ,  A61P35/00 ,  C01B32/194 ,  C07H19/10 ,  C23F1/08 ,  H01J37/20 ,  C01B2204/02 ,  C01B2204/04 ,  H01J2235/183 ,  H01J2237/20 ,  H01J2237/26

摘要： This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.

公开(公告)号：US20230012465A9

公开(公告)日：2023-01-12

申请号：US16987173

申请日：2020-08-06

申请人： Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： C23F1/18 ,  H01J37/20 ,  C01B32/194 ,  C23F1/08

摘要： This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.

公开(公告)号：US07737412B2

公开(公告)日：2010-06-15

申请号：US11571980

申请日：2005-07-12

申请人： Jian Jin ,  Robert M. Glaeser

发明人： Jian Jin ,  Robert M. Glaeser

IPC分类号： G21K5/04 ,  H01J37/24

CPC分类号： H01J37/26 ,  H01J2237/2614

摘要： A microfabricated electron phase shift element is used for modifying the phase characteristics of an electron beam passing though its center aperture, while not affecting the more divergent portion of an incident beam to selectively provide a ninety-degree phase shift to the unscattered beam in the back focal plan of the objective lens, in order to realize Zernike-type, in-focus phase contrast in an electron microscope. One application of the element is to increase the contrast of an electron microscope for viewing weakly scattering samples while in focus. Typical weakly scattering samples include biological samples such as macromolecules, or perhaps cells. Preliminary experimental images demonstrate that these devices do apply a ninety degree phase shift as expected. Electrostatic calculations have been used to determine that fringing fields in the region of the scattered electron beams will cause a negligible phase shift as long as the ratio of electrode length to the transverse feature-size aperture is about 5:1. Calculations are underway to determine the feasibility of aspect smaller aspect ratios of about 3:1 and about 2:1.

摘要翻译： 微量化电子相移元件用于改变通过其中心孔的电子束的相位特性，同时不影响入射光束的更多发散部分，以选择性地向背面的未散射光束提供九十度相移 物镜的焦点方案，以便在电子显微镜中实现Zernike型聚焦相位对比度。 该元件的一个应用是增加用于在聚焦时观察弱散射样品的电子显微镜的对比度。 典型的弱散射样品包括生物样品，如大分子，或许细胞。 初步的实验图像表明，这些设备确实应用了90度的相位偏移。 已经使用静电计算来确定，只要电极长度与横向特征尺寸孔径的比率为约5:1，散射电子束区域中的边缘场将引起可忽略的相移。 正在进行计算，以确定方面较小的纵横比为约3：1和约2：1的可行性。