公开(公告)号：US20120043208A1

公开(公告)日：2012-02-23

申请号：US13176704

申请日：2011-07-05

申请人： Jian Jin ,  Mark D. Biggin ,  Robert A. Nordmeyer ,  Ming Dong ,  Earl W. Cornell ,  Megan Choi ,  Halina Ewa Witkowski ,  Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Jian Jin ,  Mark D. Biggin ,  Robert A. Nordmeyer ,  Ming Dong ,  Earl W. Cornell ,  Megan Choi ,  Halina Ewa Witkowski ,  Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： G01N33/559 ,  C25B7/00 ,  G01N27/447

CPC分类号： G01N27/44756 ,  G01N27/44769

摘要： A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.

摘要翻译： 一种用于有效收集通过凝胶电泳分离的分子的多通道凝胶电泳装置，因此可以进一步分析，鉴定或用作试剂或药物。 该装置使用将内源性复合物的多维分离与其组成的质谱监测相结合的新型“无标记”策略。 在该方法中，基于通过多个正交分离步骤的多肽集合的共同迁移来鉴定推定的蛋白质复合物。 显示大部分大肠杆菌蛋白质通过三个色谱步骤在粗提取物分馏期间保持在稳定的络合物中。

公开(公告)号：US11708637B2

公开(公告)日：2023-07-25

申请号：US16987173

申请日：2020-08-06

申请人： Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： C23F1/18 ,  H01J37/20 ,  C01B32/194 ,  C23F1/08 ,  A61P35/00 ,  A61K9/19 ,  A61K9/51 ,  C07H19/10

CPC分类号： C23F1/18 ,  A61K9/19 ,  A61K9/5169 ,  A61P35/00 ,  C01B32/194 ,  C07H19/10 ,  C23F1/08 ,  H01J37/20 ,  C01B2204/02 ,  C01B2204/04 ,  H01J2235/183 ,  H01J2237/20 ,  H01J2237/26

摘要： This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.

公开(公告)号：US20230012465A9

公开(公告)日：2023-01-12

申请号：US16987173

申请日：2020-08-06

申请人： Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： C23F1/18 ,  H01J37/20 ,  C01B32/194 ,  C23F1/08

摘要： This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.

公开(公告)号：US20210047740A1

公开(公告)日：2021-02-18

申请号：US16987173

申请日：2020-08-06

申请人： Bong-Gyoon Han ,  Robert M. Glaeser

发明人： Bong-Gyoon Han ,  Robert M. Glaeser

IPC分类号： C23F1/18 ,  H01J37/20 ,  C01B32/194 ,  C23F1/08

摘要： This disclosure provides systems, methods, and apparatus related to graphene. In one aspect, a method includes submerging a frame support in an etching solution that is contained in a container. A growth substrate, a graphene sheet disposed on the growth substrate, and a primary support disposed on the graphene sheet is placed on a surface of the etching solution. The growth substrate is dissolved in the etching solution to leave the graphene sheet and the primary support floating on a surface of the etching solution. The etching solution in the container is replaced with a washing solution. The washing solution is removed from the container so that the graphene sheet becomes disposed on the frame support.