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公开(公告)号：US06933143B2
公开(公告)日：2005-08-23
申请号：US10374295
申请日：2003-02-25
IPC分类号： G01N33/543 , C12M3/02
CPC分类号： G01N33/54326 , Y10S435/817
摘要： An electronic controller, such as a programmed microcontroller, controls a series of pumps to automatically sequence the pumping of the individual fluids required by the ELISA procedure into a cell (or cells) necessary to produce a reporter, including the addition of o-nitrophenyl beta-D galactopyranoside substrate of the enzyme (“ONP-GP”), control the positioning of the carrier of the reporter adjacent the reporter sensor, analyze the data obtained from the reporter sensor and display the concentration of the bioagent determined from the analysis of the foregoing data. Once started, the apparatus, governed by the program, conducts the test automatically in the shortest time known to date, without the necessity for human intervention.
摘要翻译： 诸如编程的微控制器之类的电子控制器控制一系列的泵来自动地将ELISA过程所需的各种流体的泵送顺序排列到产生报道员所需的细胞（或细胞）中，包括加入邻硝基苯基β -D的吡喃半乳糖苷底物（“ONP-GP”），控制报告基因传感器附近报道载体的位置，分析报告基因传感器获得的数据，并显示从分析中确定的生物标志物的浓度 前面的数据。 一旦开始，由程序管理的装置在迄今为止所知的最短时间内自动进行测试，而不需要人为干预。
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2.发明授权Automated computer controlled reporter device for conducting imunnoassay and molecular biology procedures 有权自动计算机控制报告器装置进行免疫测定和分子生物学程序公开(公告)号：US06562209B1
公开(公告)日：2003-05-13
申请号：US09837946
申请日：2001-04-19
IPC分类号： G01N27416
CPC分类号： G01N35/00594 , G01N27/48 , G01N35/00693 , G01N35/0098 , G01N35/08 , G01N2035/1025 , Y10S435/817
摘要： An electronic controller, such as a programmed microcontroller (33), controls a series of pumps (P1-P6) to automatically sequence the pumping of the individual fluids required by the ELISA procedure into a cell (or cells) necessary to produce a reporter, control the positioning of the carrier of the reporter (32, 35) adjacent the reporter sensor (28), analyze the data obtained from the reporter sensor and display (36) the concentration of the bioagent determined from the analysis of the foregoing data. The foregoing elements automate the ELISA procedure. Once started, the apparatus, governed by the program, conducts the test automatically without the necessity for human intervention. In accordance with a specific aspect, the foregoing components may be housed in a single package for easy portability; may be battery powered or line powered.
摘要翻译： 诸如编程的微控制器（33）的电子控制器控制一系列泵（P1-P6）以自动地将ELISA过程所需的各种流体的泵送顺序排列成产生报告员所必需的一个（或多个）细胞， 控制与报告传感器（28）相邻的报告器（32,35）的载体的位置，分析从报告传感器获得的数据，并显示（36）从上述数据的分析确定的生物标志物的浓度。 上述因素使ELISA程序自动化。 一旦开始，由程序管理的装置自动执行测试，而不需要人为干预。 根据具体方面，上述组件可以容纳在单个包装中，以便于携带; 可能是电池供电或线路供电。
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公开(公告)号：US20110177583A1
公开(公告)日：2011-07-21
申请号：US11508598
申请日：2006-08-22
CPC分类号： G01N33/569 , G01N33/54366
摘要： Testing of the foot soldier or other person on the battlefield for bioagent contamination is facilitated by a computer controlled portable testing unit that combines a sink or other receptacle (1), an automated ELISA tester (3), and means to transport fluids (10, 9, 11, 15) stemming from the soldier deposited in the receptacle to the automated ELISA tester in which the analysis is performed. For external contamination detection, operation is initiated by a sensor (25) detecting the presence of a persons hands under a spout (23) and for internal contamination detection operation is initiated by the operation of a momentary operate switch (32). External contamination detection begins by washing the soldier's hands and/or face in receptacle (1), while internal contamination detection begins with the soldier spitting, coughing or sneezing into the receptacle.
摘要翻译： 通过计算机控制的便携式测试单元，将沉没器或其他容器（1），自动化ELISA测试仪（3）和输送流体（10）的装置组合起来的计算机控制的便携式测试单元便于对战场上的脚部战士或其他人进行生物学污染的测试， 9,11,15），其是从沉积在容器中的士兵到实施分析的自动ELISA测试仪。 对于外部污染检测，由传感器（25）启动操作，所述传感器检测在喷口（23）下方的人员的存在，并且通过瞬时操作开关（32）的操作启动内部污染检测操作。 外部污染检测开始于将士兵的手和/或面部清洗在容器（1）中，而内部污染检测则以士兵吐痰，咳嗽或打喷嚏到容器中开始。
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公开(公告)号：US07713752B2
公开(公告)日：2010-05-11
申请号：US10374270
申请日：2003-02-25
IPC分类号： G01N33/553 , B05D7/00
CPC分类号： G01N33/54326
摘要： Mixing of a sample containing a suspect bioagent or antigen with 1° recognition molecule coated magnetic beads in the preliminary stage of an enzyme linked immunoassay (“ELISA”) procedure is emulated by first inserting the coated magnetic beads into a non-magnetic confinement region, generating a magnetic field B along the axis of said non-magnetic confinement region to agglomerate the coated magnetic beads into a porous mass; then percolating or otherwise forcing the sample solution through said porous mass; and withdrawing the magnetic field to de-agglomerate said coated magnetic beads. In seeping or otherwise flowing through the magnetic beads the molecules of the sample link to respective magnetic beads that are coated with recognition molecules.
摘要翻译： 通过首先将涂覆的磁珠插入非磁性限制区域中，在酶联免疫测定（“ELISA”）程序的初始阶段将含有可疑生物制剂或抗原的样品与1°识别分子包被的磁珠混合， 沿着所述非磁性限制区域的轴线产生磁场B，以将涂覆的磁珠凝聚成多孔质量块; 然后渗透或以其他方式强迫样品溶液通过所述多孔质量; 并且撤回磁场以使所述涂覆的磁珠去聚集。 在渗透或以其他方式流过磁珠时，样品的分子连接到被识别分子涂覆的相应的磁珠上。
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公开(公告)号：US07081366B2
公开(公告)日：2006-07-25
申请号：US10449898
申请日：2003-06-02
IPC分类号： G01N33/553
CPC分类号： G01N33/54333 , G01N33/54326 , Y10T436/24
摘要： A primary antibody bead suspension for an enzyme-linked immunoassay (“ELISA”) procedure is formed of a quantity of primary antibody coated magnetic beads (3) uniformly dispersed and held in suspension (9) by a thixotropic non-Newtonian fluid (1). To remove the thixotropic non-Newtonian fluid prior to application in the ELISA procedure, a magnet (12) is placed against the side of the non-magnetic vessel (9) holding the suspension to draw the magnetic beads against the side while the thixotropic fluid is washed away by pumping (14,16,15& 17) and replaced by a saline buffer solution.
摘要翻译： 通过触变非牛顿流体（1）均匀分散并保持在悬浮液（9）中的一定量的一级抗体包被的磁珠（3）形成用于酶联免疫测定（“ELISA”）程序的一级抗体珠悬浮液， 。 为了在施用ELISA程序之前去除触变非牛顿流体，将磁体（12）放置在非磁性容器（9）的保持悬浮液的侧面以将磁珠拉向侧面，而触变流体 通过泵送（14,16,15和17）洗去并用盐水缓冲溶液代替。
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公开(公告)号：US06803202B2
公开(公告)日：2004-10-12
申请号：US10055318
申请日：2001-10-23
IPC分类号： G01N33543
CPC分类号： G01N33/54313 , G01N33/53 , G06F19/00 , G16H50/80
摘要： The existence of any one of a plurality of 2N−1 bioagents in a sample (1) may be determined by dividing the sample into N parts and performing separate ELISA processes (TEST 1 & TEST 2) on each of the N parts concurrently, where N is an integer greater than 1, each of the separate ELISA processes possessing the capability to identify a bioagent from a combination of possible bioagents (3), and in which the combination of possible bioagents of any one of the separate ELISA processes is different (7) from the combination of possible bioagents of any other of the separate ELISA processes.
摘要翻译： 可以通过将样品分成N个部分并在N个部分中的每一个上进行单独的ELISA过程（TEST 1＆TEST 2）来确定样品（1）中多个2 N-1个生物体中任何一个的存在 同时，其中N是大于1的整数，每个单独的ELISA方法具有从可能的生物标签（3）的组合鉴定生物标签的能力，并且其中任何一个单独的ELISA过程的可能的生物标签的组合 与来自任何其他单独ELISA过程的可能的生物标记的组合不同（7）。
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7.发明授权Forwarding traffic to VLAN interfaces built based on subscriber information strings 有权将流量转发到基于用户信息字符串构建的VLAN接口公开(公告)号：US07808994B1
公开(公告)日：2010-10-05
申请号：US11540923
申请日：2006-09-29
申请人： Vitali Vinokour , Linda M. Cabeca , Karen Ruben , Steve Onishi , Christopher Roche , Brian M. Sullivan
发明人： Vitali Vinokour , Linda M. Cabeca , Karen Ruben , Steve Onishi , Christopher Roche , Brian M. Sullivan
CPC分类号： H04L12/4645 , H04L45/52
摘要： Techniques are described that allow a network device, such as a router, to forward data packets received from a subscriber device to an Ethernet virtual local area network (VLAN) interface within the network device where the VLAN interface has been dynamically built based on a subscriber information string. For example, a primary VLAN sub-interface (PVS) and a subscriber VLAN sub-interface (SVS) may each be dynamically built over a statically built VLAN major interface. In particular, the network device comprises a forwarding controller, where the forwarding controller receives a data packet over an Ethernet port. The network device accesses upper-layer protocol information within the data packet to determine an SVS within the network device to which to forward the data packet based on the upper-layer protocol information.
摘要翻译： 描述了允许诸如路由器的网络设备将从订户设备接收的数据分组转发到网络设备内的以太网虚拟局域网（VLAN）接口的技术，其中VLAN接口已经基于用户动态构建 信息字符串。 例如，主VLAN子接口（PVS）和用户VLAN子接口（SVS）可以分别通过静态构建的VLAN主接口动态构建。 具体地，网络设备包括转发控制器，转发控制器通过以太网端口接收数据分组。 网络设备访问数据包内的上层协议信息，以确定基于上层协议信息转发数据包的网络设备内的SVS。
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公开(公告)号：US20090205024A1
公开(公告)日：2009-08-13
申请号：US12030056
申请日：2008-02-12
申请人： Mathias Kokot , Sanjay Wadhwa , Brian M. Sullivan
发明人： Mathias Kokot , Sanjay Wadhwa , Brian M. Sullivan
CPC分类号： H04L63/08 , H04L12/2869 , H04L12/4641 , H04L12/4654
摘要： Methods and systems consistent with the present invention provide a dynamic mechanism to support wholesale access for broadband subscribers. This mechanism involves dynamically discovering a retail ISP for a subscriber, and dynamically cross-connecting a subscriber's connection to a logical connection corresponding to a retail ISP, and is equally applicable to static, PPP and DHCP-based subscribers. Furthermore, dynamic steering of subscribers can be performed at layer 2 or layer 3 of the OSI model.
摘要翻译： 与本发明一致的方法和系统提供了支持宽带用户的批发接入的动态机制。 该机制涉及动态地发现用户的零售ISP，并将用户的连接动态地交换到对应于零售ISP的逻辑连接，并且同样适用于静态，基于PPP和基于DHCP的用户。 此外，可以在OSI模型的层2或层3上执行用户的动态转向。
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公开(公告)号：US07319039B2
公开(公告)日：2008-01-15
申请号：US10985602
申请日：2004-11-09
申请人： Brian M. Sullivan
发明人： Brian M. Sullivan
IPC分类号： G01N21/76
CPC分类号： G01N21/6428 , G01N2001/022 , G01N2021/1793 , G01N2021/6432 , Y10T436/25875
摘要： The presence of an aerosol or cloud (3) of a toxic substance is detected by producing an aerosol (17, 5) of ligands (13) to the target substance in a region of sky that contains the target substance, permitting molecules the target substance to bind to the ligand, directing light (7, 21) of a first frequency into the revised aerosol; and then inspecting (9, 25) the revised aerosol for emissions of light of a second frequency. When light of the second frequency exists an alarm (15, 31) is initiated.
摘要翻译： 通过在包含目标物质的天空区域中产生与目标物质的配体（13）的气溶胶（17,5）来检测有毒物质的气溶胶或云（3）的存在，允许将目标物质分子 将第一频率的光（7,21）引导到修改的气溶胶中; 然后检查（9,25）用于第二频率的光的修正气溶胶。 当存在第二频率的光时，发起报警（15,31）。
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公开(公告)号：US20240193702A1
公开(公告)日：2024-06-13
申请号：US18538904
申请日：2023-12-13
申请人： Brian M. Sullivan
发明人： Brian M. Sullivan
IPC分类号： G06Q50/00
CPC分类号： G06Q50/01
摘要： A social media platform accessed online by platform user devices forming a collection of interactive media technologies that facilitate the creation and sharing of user created content of information, ideas, interests, and other forms of expression through virtual communities and networks of the user devices. The platform provides a predefined set of sentiment identifiers which are bindable to user defined content components of the user created content; wherein the bound content-sentiment pairings are indexable, searchable and analyzable by the platform or users on the platform. Platform AI systems index search and analyze the bound content-sentiment pairings of user content.
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