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1.发明申请Fermentive Production of Isobutanol Using Highly Effective Ketol-Acid Reductoisomerase Enzymes 审中-公开使用高效酮酯酸重排酶酶的异丁醇发酵生产公开(公告)号：US20160319307A1
公开(公告)日：2016-11-03
申请号：US15209841
申请日：2016-07-14
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/88 , C12N15/52 , C12P7/42 , C12Y101/01008 , C12Y101/01086 , C12Y401/01001 , C12Y401/01072 , C12Y401/02009 , C12Y402/01009 , Y02E50/10 , Y02P20/52
摘要： Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.
摘要翻译： 已经鉴定了酮醇酸还原异构酶，其在细菌和酵母中作为异丁醇生物合成途径的步骤在体内提供高效力。 这些KARI是通过称为SLSL分子的分子系统发育分析鉴定的进化枝的成员。
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2.发明申请Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof 审中-公开具有Fe-S簇蛋白的重组酵母宿主细胞及其使用方法公开(公告)号：US20160222370A1
公开(公告)日：2016-08-04
申请号：US15016759
申请日：2016-02-05
发明人： Larry Cameron ANTHONY , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous bacterial, fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源细菌，真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5 ），异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US10308964B2
公开(公告)日：2019-06-04
申请号：US15340084
申请日：2016-11-01
发明人： Dennis Flint , Brian James Paul , Rick W. Ye
IPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , G01N33/573 , C12N15/81
摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
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公开(公告)号：US10287566B2
公开(公告)日：2019-05-14
申请号：US15406876
申请日：2017-01-16
发明人： Kristen J. Kelly , Rick W. Ye
摘要： Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
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公开(公告)号：US20140273116A1
公开(公告)日：2014-09-18
申请号：US14207823
申请日：2014-03-13
发明人： Kristen J. Kelly , Rick W. Ye
摘要： Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
摘要翻译： 公开了显示增加的DHAD活性的二羟基脱水酶（DHAD）变体的筛选方法以及通过这些方法鉴定的DHAD变体。 这样的酶可导致需要生物合成途径的DHAD生成化合物。 还公开了编码DHAD变体的分离的核酸，包含分离的核酸分子的重组宿主细胞和生产丁醇的方法。
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公开(公告)号：US09909149B2
公开(公告)日：2018-03-06
申请号：US15485735
申请日：2017-04-12
CPC分类号： C12P7/16 , C07K2319/00 , C12N9/88 , C12N15/00 , C12P7/40 , C12Y402/01009 , Y02E50/10
摘要： Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
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公开(公告)号：US09580705B2
公开(公告)日：2017-02-28
申请号：US14207823
申请日：2014-03-13
发明人： Kristen J. Kelly , Rick W. Ye
摘要： Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
摘要翻译： 公开了显示增加的DHAD活性的二羟基脱水酶（DHAD）变体的筛选方法以及通过这些方法鉴定的DHAD变体。 这样的酶可导致需要生物合成途径的DHAD生成化合物。 还公开了编码DHAD变体的分离的核酸，包含分离的核酸分子的重组宿主细胞和生产丁醇的方法。
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公开(公告)号：US20170101653A1
公开(公告)日：2017-04-13
申请号：US15340084
申请日：2016-11-01
发明人： Dennis Flint , Brian James Paul , Rick W. Ye
IPC分类号： C12P7/16 , C12N15/81 , C07K14/395 , C12N9/88
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12N15/81 , C12P7/40 , C12P13/02 , C12P13/04 , C12Q1/527 , C12Y402/01009 , G01N33/573 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
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