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    Methods, Devices and Systems for Algae Lysis and Content Extraction
    4.
    发明申请
    Methods, Devices and Systems for Algae Lysis and Content Extraction 审中-公开
    藻类裂解和含量提取的方法，装置和系统

    公开(公告)号：US20160122701A1

    公开(公告)日：2016-05-05

    申请号：US14764906

    申请日：2014-01-31

    Applicant: CARNEGIE MELLON UNIVERSITY

    Inventor： C. Fred Higgs, III ,  Philip R. LeDuc ,  Kristin M. Warren ,  Jeremiah N. Mpagazehe

    IPC: C12M1/42 ,  C12N1/12 ,  G06F17/50 ,  C12N1/06

    CPC classification number: C12M35/04 ,  C12N1/066 ,  C12N1/12 ,  G06F17/5009

    Abstract: Described herein are devices, systems and methods for lysing algae cells, for production of a lysate product such as a biofuel. The systems and methods use a passive device that lyses the cells through flow configurations, geometries, and surfaces that would induce different stresses and negative pressure on the microalgae cells. When the stress is designed to exceed the mechanical strength of the microalgae cells, the cells are lysed, causing, e.g., lipid release which can be used to produce biofuels. Through an internally-created computational framework, the concept is validated and can be optimized for the lowest energy input with the highest level of lipid release. Also provided herein are computer-implemented methods for optimizing lysis in such systems and computer-readable media containing instructions for performing the computer-implemented methods.

    Abstract translation: 本文描述了用于裂解藻类细胞的装置，系统和方法，用于生产裂解产物如生物燃料。 系统和方法使用无源器件，其通过将在微藻细胞上诱导不同应力和负压的流动配置，几何形状和表面来裂解细胞。 当应力被设计成超过微藻细胞的机械强度时，细胞被裂解，引起例如可用于生产生物燃料的脂质释放。 通过内部创建的计算框架，该概念得到验证，并且可以针对具有最高脂质释放水平的最低能量输入进行优化。 本文还提供了用于优化这种系统中的裂解的计算机实现的方法和包含用于执行计算机实现的方法的指令的计算机可读介质。

    POLYCARBONATE HEAT MOLDING FOR SOFT LITHOGRAPHY
    5.
    发明申请
    POLYCARBONATE HEAT MOLDING FOR SOFT LITHOGRAPHY 有权

    公开(公告)号：US20220269168A1

    公开(公告)日：2022-08-25

    申请号：US17681676

    申请日：2022-02-25

    Applicant: CARNEGIE MELLON UNIVERSITY

    Inventor： Philip R. LeDuc ,  Utku Sonmez

    IPC: G03F7/00

    Abstract: A method of replicating master molds used in the fabrication of microsystems having micron to millimeter sized features. Master molds are replicating using a polymer sheet, which is heated and melted onto an elastomeric mold fabricated from the master mold. The copy molds accurately replicate the geometries of the master mold, such as high aspect ratio features, microposts, and channels with slender sidewalls. The polymer sheet encases the elastomeric mold without the application of an external force, permitting copying without deformation of the features.

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