公开(公告)号：US20170342465A1

公开(公告)日：2017-11-30

申请号：US15608780

申请日：2017-05-30

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2521/107 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise.

公开(公告)号：US11932849B2

公开(公告)日：2024-03-19

申请号：US16677012

申请日：2019-11-07

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12N15/10 ,  C40B40/06 ,  C40B50/04 ,  C40B50/06 ,  C40B70/00

CPC classification number: C12N15/1096 ,  C12N15/1006 ,  C40B40/06 ,  C40B50/04 ,  C40B50/06 ,  C40B70/00

Abstract: Disclosed herein include systems, methods, compositions, and kits for whole transcriptome analysis (WTA) with random priming and extension (RPE). The RPE-based WTA method can comprise hybridizing random primers with a plurality of first strand barcoded polynucleotides associated with a solid support and extending the random primers to generate a plurality of extension products. The method can comprise amplifying the plurality of extension products to generate a sequencing library.

公开(公告)号：US11492660B2

公开(公告)日：2022-11-08

申请号：US16707780

申请日：2019-12-09

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12Q1/6837 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: Disclosed herein include methods and compositions for selectively amplifying and/or extending nucleic acid target molecules in a sample. The methods and compositions can, for example, reduce the amplification and/or extension of undesirable nucleic acid species in the sample, and/or allow selective removal of undesirable nucleic acid species in the sample.

公开(公告)号：US10202641B2

公开(公告)日：2019-02-12

申请号：US15608780

申请日：2017-05-30

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12Q1/68 ,  C12Q1/686

Abstract: Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise.

公开(公告)号：US20180216174A1

公开(公告)日：2018-08-02

申请号：US15875816

申请日：2018-01-19

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn K. Fu

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6832

CPC classification number: C12Q1/6853 ,  C12N15/1093 ,  C12N2310/3181 ,  C12Q1/6806 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q2563/159 ,  C12Q2525/186 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q2565/537

Abstract: Disclosed herein include methods and compositions for selectively amplifying and/or extending nucleic acid target molecules in a sample. The methods and compositions can, for example, reduce the amplification and/or extension of undesirable nucleic acid species in the sample, and/or allow selective removal of undesirable nucleic acid species in the sample.

公开(公告)号：US20180088112A1

公开(公告)日：2018-03-29

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  C12Q1/68 ,  G01N33/543 ,  C12N15/10

CPC classification number: G01N33/566 ,  B01J2219/00646 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07K2319/01 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/505 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  G06F19/00 ,  G16B25/10 ,  G16B99/00 ,  G16H10/20 ,  G16H20/13 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20170314067A1

公开(公告)日：2017-11-02

申请号：US15581914

申请日：2017-04-28

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu

IPC: C12Q1/68

Abstract: In accordance with some embodiments herein, compositions and methods for accurate barcoding of nucleic acids are described. The compositions and methods can involve a plurality of unique oligonucleotide species comprising unique molecule barcodes. In some embodiments, the molecule barcodes can have a relatively low G content, and can exhibit reduced bias in amplification and analysis.

公开(公告)号：US11525157B2

公开(公告)日：2022-12-13

申请号：US16232287

申请日：2018-12-26

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12P19/34 ,  C12Q1/686

Abstract: Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise.

公开(公告)号：US11319583B2

公开(公告)日：2022-05-03

申请号：US15875816

申请日：2018-01-19

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn K. Fu

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6832 ,  C12Q1/6806 ,  C12N15/10

Abstract: Disclosed herein include methods and compositions for selectively amplifying and/or extending nucleic acid target molecules in a sample. The methods and compositions can, for example, reduce the amplification and/or extension of undesirable nucleic acid species in the sample, and/or allow selective removal of undesirable nucleic acid species in the sample.

公开(公告)号：US10822643B2

公开(公告)日：2020-11-03

申请号：US15581914

申请日：2017-04-28

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: In accordance with some embodiments herein, compositions and methods for accurate barcoding of nucleic acids are described. The compositions and methods can involve a plurality of unique oligonucleotide species comprising unique molecule barcodes. In some embodiments, the molecule barcodes can have a relatively low G content, and can exhibit reduced bias in amplification and analysis.