公开(公告)号：US11279752B2

公开(公告)日：2022-03-22

申请号：US16559561

申请日：2019-09-03

申请人： City of Hope ,  Thomas Jefferson University

发明人： John C. Williams ,  Ulrich Rodeck ,  Kurt Jenkins

IPC分类号： C07K14/82 ,  C07K16/28 ,  C07K14/47 ,  C07K14/705 ,  C07K14/71 ,  C12N9/64

摘要： Provided herein are recombinant masking proteins and recombinant ligand proteins useful in treating cancer, neurodegenerative disease, and cardiovascular disease. The recombinant masking proteins provided herein may, inter alia, be used as non-covalent masks of antagonists of, for example, cellular growth factors (e.g., TNF) or cell surface proteins (e.g., CTLA-4).

公开(公告)号：US10414814B2

公开(公告)日：2019-09-17

申请号：US15322438

申请日：2015-07-02

申请人： City of Hope ,  Thomas Jefferson University

发明人： John C. Williams ,  Ulrich Rodeck ,  Kurt Jenkins

IPC分类号： C07K14/82 ,  C07K16/46 ,  C07K14/705 ,  C12N9/64 ,  C07K14/435 ,  C07K19/00 ,  C07K16/28 ,  C07K14/47 ,  C07K14/71

摘要： Provided herein are recombinant masking proteins and recombinant ligand proteins useful in treating cancer, neurodegenerative disease, and cardiovascular disease. The recombinant masking proteins provided herein may, inter alia, be used as non-covalent masks of antagonists of, for example, cellular growth factors (e.g., TNF) or cell surface proteins (e.g., CTLA-4).

公开(公告)号：US20230085485A1

公开(公告)日：2023-03-16

申请号：US17660603

申请日：2022-04-25

申请人： CITY OF HOPE ,  THOMAS JEFFERSON UNIVERSITY

发明人： John C. Williams ,  Cindy Zer ,  Kendra N. Avery ,  Ulrich Rodeck ,  Joshua M. Donaldson ,  Csaba Kari

IPC分类号： A61K47/55 ,  C07K16/28 ,  C07K16/30

摘要： In one embodiment, a masked monoclonal antibody (mAb) is provided, the mAb, encoded by a nucleic acid sequence or an amino acid sequence molecule comprising a signal sequence, a masking epitope sequence, a linker sequence that is cleavable by a protease specific to a target tissue; and an antibody or a functional fragment thereof. In another embodiment, a masked monoclonal antibody (mAb) is provided, which includes a therapeutic mAb and a mask, the mask comprising protein A and protein L attached by a protease cleavable linker.

公开(公告)号：US11311622B2

公开(公告)日：2022-04-26

申请号：US16460957

申请日：2019-07-02

申请人： CITY OF HOPE ,  Thomas Jefferson University

发明人： John C. Williams ,  Cindy Zer ,  Kendra N. Avery ,  Ulrich Rodeck ,  Joshua M. Donaldson ,  Csaba Kari

IPC分类号： A61K47/55 ,  C07K16/28 ,  C07K16/30 ,  C07K16/22

摘要： In one embodiment, a masked monoclonal antibody (mAb) is provided, the mAb, encoded by a nucleic acid sequence or an amino acid sequence molecule comprising a signal sequence, a masking epitope sequence, a linker sequence that is cleavable by a protease specific to a target tissue; and an antibody or a functional fragment thereof. In another embodiment, a masked monoclonal antibody (mAb) is provided, which includes a therapeutic mAb and a mask, the mask comprising protein A and protein L attached by a protease cleavable linker.

公开(公告)号：US10357571B2

公开(公告)日：2019-07-23

申请号：US14949648

申请日：2015-11-23

申请人： City of Hope ,  Thomas Jefferson University

发明人： John C. Williams ,  Cindy Zer ,  Kendra N. Avery ,  Ulrich Rodeck ,  Joshua M. Donaldson ,  Csaba Kari

IPC分类号： C07K16/28 ,  C07K16/30 ,  A61K47/48 ,  A61K47/55

摘要： In one embodiment, a masked monoclonal antibody (mAb) is provided, the mAb, encoded by a nucleic acid sequence or an amino acid sequence molecule comprising a signal sequence, a masking epitope sequence, a linker sequence that is cleavable by a protease specific to a target tissue; and an antibody or a functional fragment thereof. In another embodiment, a masked monoclonal antibody (mAb) is provided, which includes a therapeutic mAb and a mask, the mask comprising protein A and protein L attached by a protease cleavable linker.

公开(公告)号：US11186642B2

公开(公告)日：2021-11-30

申请号：US15439823

申请日：2017-02-22

申请人： City of Hope ,  Thomas Jefferson University

发明人： John C. Williams ,  Ulrich Rodeck ,  Joshua M. Donaldson ,  Csaba Kari

IPC分类号： C07K16/28 ,  C07K16/30 ,  C07K16/32

摘要： In one embodiment, a masked monoclonal antibody (mAb) is provided, the mAb, encoded by a nucleic acid sequence or an amino acid sequence molecule comprising a signal sequence, a masking epitope sequence, a linker sequence that is cleavable by a protease specific to a target tissue; and an antibody or a functional fragment thereof. In another embodiment, a cross-masked mAb heterodimer complex is provided, comprising a first masked mAb, comprising a first signal sequence, a first masking epitope sequence, a first linker that is cleavable by a protease specific to a target tissue, and a first antibody or fragment thereof; and a second masked mAb, comprising a second signal sequence, a second masking epitope sequence, a second linker that is cleavable by a protease specific to a target tissue, and a second antibody or fragment thereof.

公开(公告)号：US20240228629A1

公开(公告)日：2024-07-11

申请号：US18277218

申请日：2022-01-14

申请人： City of Hope

发明人： Alfur Hung ,  Miso Park ,  Yi-Chiu Kuo ,  John C. Williams

IPC分类号： C07K16/28 ,  A61K39/00 ,  A61P35/00

CPC分类号： C07K16/2863 ,  A61P35/00 ,  A61K2039/505 ,  C07K2317/565

摘要： Provided herein are, inter alia, novel antibodies that specifically bind to domain IV of EGFR and are able to effectively induce antibody dependent cell mediated cytoxicity (ADCC) against EGFR-expressing cells. In embodiments, the immunoglobulin and the Fab of the antibodies provided herein bind domain IV of EGFR with differential affinity. Thus, the antibodies provided herein provide for highly specific antibody therapeutics without adverse effects.

公开(公告)号：US20200164077A1

公开(公告)日：2020-05-28

申请号：US16460957

申请日：2019-07-02

申请人： CITY OF HOPE

发明人： John C. Williams ,  Cindy Zer ,  Kendra N. Avery ,  Ulrich Rodeck ,  Joshua M. Donaldson ,  Csaba Kari

IPC分类号： A61K47/55 ,  C07K16/30 ,  C07K16/28

摘要： In one embodiment, a masked monoclonal antibody (mAb) is provided, the mAb, encoded by a nucleic acid sequence or an amino acid sequence molecule comprising a signal sequence, a masking epitope sequence, a linker sequence that is cleavable by a protease specific to a target tissue; and an antibody or a functional fragment thereof. In another embodiment, a masked monoclonal antibody (mAb) is provided, which includes a therapeutic mAb and a mask, the mask comprising protein A and protein L attached by a protease cleavable linker.

公开(公告)号：US09260710B2

公开(公告)日：2016-02-16

申请号：US14754064

申请日：2015-06-29

申请人： City of Hope

发明人： John C. Williams ,  David Horne ,  Jun Xie ,  Shubbir Ahmed

IPC分类号： C12N9/96 ,  C12N9/90 ,  C07D211/60 ,  G01N33/50

CPC分类号： C12N9/90 ,  C07D211/60 ,  C12N9/93 ,  C12N9/96 ,  C12Y502/01 ,  C12Y502/01008 ,  G01N33/5023 ,  G01N2333/4703 ,  G01N2333/914

摘要： There are provided, inter alia, photolabile compounds and methods useful for the formation of dimers of biological molecules and subsequent dissociation of the dimers.

公开(公告)号：US12060435B2

公开(公告)日：2024-08-13

申请号：US16959981

申请日：2019-01-07

申请人： CITY OF HOPE

发明人： John C. Williams ,  Jeremy King

IPC分类号： C07K16/18 ,  A61K47/65 ,  A61P35/00 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  C12N5/0783

CPC分类号： C07K16/32 ,  A61K47/65 ,  A61P35/00 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/283 ,  C07K16/2866 ,  C07K16/2875 ,  C07K16/2878 ,  C07K16/2887 ,  C07K16/3092 ,  C12N5/0636 ,  C07K2317/31 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/52 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/55 ,  C07K2317/569 ,  C07K2317/64

摘要： Provided herein are, inter alia, novel peptide compositions having multi-specific binding capabilities useful for therapeutic and diagnostic purposes. The peptide compositions provided herein are polypeptide conjugates including at least two ligand binding domains able to target (bind) two or more ligands (e.g., antigens) at the same time. The peptide compositions provided herein can be produced at very high yields and are therefore easy to manufacture.