公开(公告)号：US12168229B2

公开(公告)日：2024-12-17

申请号：US16953601

申请日：2020-11-20

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES ,  Institut National de la Sante et de la recherche Medicale

Inventor： Fabrice Casset ,  Arnaud Millet ,  Baptiste Neff

IPC: B01L3/00 ,  G01N15/14 ,  G01N15/149

Abstract: Device for locating biological cells of at least one size, including a fluid cavity (3), fluid connection means (6.1, 6.2) configured to allow the introduction of liquid and cells into the fluid cavity, a plate (4) provided with an accommodating surface (8) having properties of adhesion vis-à-vis cells, said plate (4) being suspended in the fluid cavity (3), means (10) for vibrating the plate (4) in at least one stationary mode including at least one actuator (16) intended to act on the plate (4) and a control unit (UC) configured to control the at least one actuator so as to actuate the vibrating plate in a stationary mode at a wavelength greater than the size of the cells.

公开(公告)号：US10538731B2

公开(公告)日：2020-01-21

申请号：US15046803

申请日：2016-02-18

Applicant: Commissariat a L'Energie Atomique et aux Energies Alternatives

Inventor： Fabrice Casset ,  Arnaud Millet

IPC: C12M1/00 ,  C12M3/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/26 ,  C12M1/12

Abstract: Device for sorting living cells, comprising at least one support including a surface having adherence properties with respect to said type of living cells, actuators capable of making said surface vibrate at at least one given frequency and a controller for controlling the actuators such that the surface vibrates at a frequency causing the detachment of at least one type of living cells.

公开(公告)号：US20170121661A1

公开(公告)日：2017-05-04

申请号：US15335702

申请日：2016-10-27

Applicant: Commissariat A L'Energie Atomique et aux Energies Alternatives

Inventor： Fabrice CASSET ,  Arnaud Millet

IPC: C12M1/42 ,  C12N13/00

CPC classification number: C12M35/02 ,  C12M35/00 ,  C12M35/04 ,  C12N13/00

Abstract: Device for treating at least one biological cell comprising: at least one treatment support comprising a receiving surface enabling the adhesion of the at least one biological cell, at least one actuator capable of deforming said support in order to apply a stress to said at least one cell, and means of controlling said at least one actuator such that the actuator deforms the treatment support according to a given amplitude of deformation and for a given duration so as to generate at least one transitory pore in a membrane of the biological cell.

公开(公告)号：US12179198B2

公开(公告)日：2024-12-31

申请号：US16475901

申请日：2018-01-05

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES

Inventor： Vincent Agache ,  Fabrice Casset ,  Stephane Fanget ,  Yves Fouillet ,  Arnaud Millet

IPC: C12Q1/04 ,  B01L3/00 ,  C12M1/26 ,  G01N15/01 ,  G01N15/10

Abstract: A system for handling biological cells includes a main channel having an inlet end and an outlet end, and, at least over a first portion from the inlet end, including a cross section such that a cell circulating in the portion undergoes mechanical stresses. The system further includes first means for detecting the presence of a cell at the inlet end of the main channel and at least one access zone opening into the main channel between its inlet end and its outlet end in the first portion, in order to make it possible to exert an action on the cell. The system also includes means for displacing the cell in order to control the displacement of the cell between the inlet end and the outlet end.

公开(公告)号：US10968422B2

公开(公告)日：2021-04-06

申请号：US15335702

申请日：2016-10-27

Applicant: Commissariat A L'Energie Atomique et aux Energies Alternatives ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

Inventor： Fabrice Casset ,  Arnaud Millet

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/42 ,  C12N13/00

Abstract: A device for treating at least one biological cell includes at least one treatment support comprising a receiving surface enabling the adhesion of the at least one biological cell, at least one actuator capable of deforming said support in order to apply a stress to said at least one cell, and means of controlling said at least one actuator such that the actuator deforms the treatment support according to a given amplitude of deformation and for a given duration so as to generate at least one transitory pore in a membrane of the biological cell.