公开(公告)号：US10336805B2

公开(公告)日：2019-07-02

申请号：US15988802

申请日：2018-05-24

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C07K14/705 ,  C12N15/85 ,  A01N1/02 ,  G01N33/50

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US10017558B2

公开(公告)日：2018-07-10

申请号：US15688942

申请日：2017-08-29

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C07K14/705 ,  G01N33/50 ,  A01N1/02 ,  C12N15/85

CPC classification number: C07K14/705 ,  A01N1/021 ,  A01N1/0284 ,  C12N5/00 ,  C12N5/067 ,  C12N5/069 ,  C12N15/85 ,  C12N2510/00 ,  G01N33/5008 ,  G01N2333/705

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US20200239540A1

公开(公告)日：2020-07-30

申请号：US16851649

申请日：2020-04-17

Applicant: CORNING INCORPORATED

Inventor： Na Li ,  Christopher Jerome Patten ,  Jie Wang

IPC: C07K14/705 ,  G05B15/02 ,  G08B13/00 ,  G08B13/196 ,  G08B25/00 ,  G08B25/08 ,  G08B29/18 ,  C12N15/85 ,  A01N1/02 ,  C12N5/00 ,  C12N5/071 ,  G01N33/50

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US10047136B2

公开(公告)日：2018-08-14

申请号：US15688983

申请日：2017-08-29

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C07K14/705 ,  G01N33/50 ,  A01N1/02 ,  C12N15/85

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US20170355745A1

公开(公告)日：2017-12-14

申请号：US15688942

申请日：2017-08-29

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C07K14/705 ,  G01N33/50 ,  C12N5/00 ,  C12N15/85 ,  A01N1/02 ,  C12N5/071

CPC classification number: C07K14/705 ,  A01N1/021 ,  A01N1/0284 ,  C12N5/00 ,  C12N5/067 ,  C12N5/069 ,  C12N15/85 ,  C12N2510/00 ,  G01N33/5008 ,  G01N2333/705

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US09822160B2

公开(公告)日：2017-11-21

申请号：US14972012

申请日：2015-12-16

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C07K14/705 ,  C12N15/85 ,  A01N1/02 ,  G01N33/50

CPC classification number: C07K14/705 ,  A01N1/021 ,  A01N1/0284 ,  C12N5/00 ,  C12N5/067 ,  C12N5/069 ,  C12N15/85 ,  C12N2510/00 ,  G01N33/5008 ,  G01N2333/705

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US20160264641A1

公开(公告)日：2016-09-15

申请号：US15163218

申请日：2016-05-24

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C07K14/705 ,  G01N33/50 ,  C12N5/00 ,  A01N1/02 ,  C12N5/071

CPC classification number: C07K14/705 ,  A01N1/021 ,  A01N1/0284 ,  C12N5/00 ,  C12N5/067 ,  C12N5/069 ,  C12N15/85 ,  C12N2510/00 ,  G01N33/5008 ,  G01N2333/705

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US20220073868A1

公开(公告)日：2022-03-10

申请号：US17422490

申请日：2020-01-10

Applicant: CORNING INCORPORATED

Inventor： Kirsten Lee Cooper ,  Jie Wang ,  Rongjun Zuo

IPC: C12N5/00 ,  C12N15/87

Abstract: A method of culturing adherent cells in suspension is provided that includes culturing adherent cells on a first substrate in a first suspension, harvesting the adherent cells from the first substrate, and transfecting the harvested adherent cells using electro-poration. The method also includes, after the step of transfecting, suspending the transfected adherent cells in a second suspension. A dissolution process for dissolving the second microcarrier particle to harvest the cells or cell products is also provided. This dissolution process includes adding a chelator, such as EDTA, to the second suspension for a predetermined time to separate the cells from the second microcarrier; and isolating the cells or cell products from a remainder of the second suspension after the predetermined time. The dissolution process is performed without enzymes such as pectinase or protease.

公开(公告)号：US20190263885A1

公开(公告)日：2019-08-29

申请号：US16407943

申请日：2019-05-09

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C07K14/705 ,  C12N5/071 ,  A01N1/02 ,  G01N33/50 ,  C12N5/00 ,  C12N15/85

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

公开(公告)号：US20180265563A1

公开(公告)日：2018-09-20

申请号：US15988819

申请日：2018-05-24

Applicant: Corning Incorporated

Inventor： Na Li ,  Jie Wang ,  Christopher J. Patten

IPC: C07K14/705 ,  C12N5/071 ,  A01N1/02 ,  C12N15/85 ,  C12N5/00 ,  G01N33/50

CPC classification number: C07K14/705 ,  A01N1/021 ,  A01N1/0284 ,  C12N5/00 ,  C12N5/067 ,  C12N5/069 ,  C12N15/85 ,  C12N2510/00 ,  G01N33/5008 ,  G01N2333/705

Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.