公开(公告)号：US20060078906A1

公开(公告)日：2006-04-13

申请号：US11142720

申请日：2005-05-31

申请人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Lao ,  Neil Straus

发明人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Lao ,  Neil Straus

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C07H21/04 ,  C12N9/00 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

公开(公告)号：US20130011840A1

公开(公告)日：2013-01-10

申请号：US13612485

申请日：2012-09-12

申请人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

发明人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C07H21/04 ,  C12N9/00 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

摘要翻译： 本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。 包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。 可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。 在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。 然后可以在多个扩增反应中解码多个查询的miRNA。

公开(公告)号：US20100112573A1

公开(公告)日：2010-05-06

申请号：US12543466

申请日：2009-08-18

申请人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

发明人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C07H21/04 ,  C12N9/00 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

摘要翻译： 本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。 包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。 可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。 在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。 然后可以在多个扩增反应中解码多个查询的miRNA。

公开(公告)号：US09068222B2

公开(公告)日：2015-06-30

申请号：US12543466

申请日：2009-08-18

申请人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

发明人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

IPC分类号： C07H21/04 ,  C12N9/00 ,  C12Q1/68

CPC分类号： C12Q1/6876 ,  C07H21/04 ,  C12N9/00 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

摘要翻译： 本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。 包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。 可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。 在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。 然后可以在多个扩增反应中解码多个查询的miRNA。

公开(公告)号：US07601495B2

公开(公告)日：2009-10-13

申请号：US11142720

申请日：2005-05-31

申请人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Q. Lao ,  Neil A. Straus

发明人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Q. Lao ,  Neil A. Straus

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C07H21/04 ,  C12N9/00 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

摘要翻译： 本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。 包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。 可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。 在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。 然后可以在多个扩增反应中解码多个查询的miRNA。

公开(公告)号：US07575863B2

公开(公告)日：2009-08-18

申请号：US10947460

申请日：2004-09-21

申请人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

发明人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C07H21/04 ,  C12N9/00 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2600/16 ,  C12Q2600/178

摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

公开(公告)号：US20140038241A1

公开(公告)日：2014-02-06

申请号：US13958553

申请日：2013-08-03

申请人： Zhaohui Zhou ,  Qun Shan

发明人： Zhaohui Zhou ,  Qun Shan

IPC分类号： C12P19/34 ,  C12N9/16

CPC分类号： C12P19/34 ,  C12N9/16 ,  C12N9/22 ,  C12Q1/6806 ,  C12Y301/21 ,  C12Q2521/301 ,  C12Q2521/543 ,  C12Q2522/101

摘要： By using engineered sequence specific DNA nuclease (“SSDN”), the composition, reagent kit and method of the present invention can cut and release a DNA sequence of interest 1×104-1×107-base pairs long from a source DNA as large as the whole genome. The SSDN further includes an affinity tag or is bound to a solid support that facilitates the isolation of the DNA sequence of interest. The SSDN can include a RecA and Ref combination, a transcription activator like effector nuclease, or a sequence specific chemical nuclease. When applied to genomic sequencing, specific region(s) of interest in the genome can be cut and isolated. Because the irrelevant part of the genome is removed from the sequencing reaction, the speed, cost, and accuracy of genomic sequencing can be improved.

摘要翻译： 通过使用工程序列特异性DNA核酸酶（“SSDN”），本发明的组合物，试剂盒和方法可以从源DNA中切下并释放出1×104-1×107碱基对长的DNA序列 作为全基因组。 SSDN还包括亲和标签或者结合固体支持物，其有利于分离所关注的DNA序列。 SSDN可以包括RecA和Ref组合，转录激活物如效应核酸酶或序列特异性化学核酸酶。 当应用于基因组测序时，可以切割和分离基因组中感兴趣的特定区域。 因为从测序反应中去除了基因组的不相关部分，因此可以提高基因组测序的速度，成本和准确性。

公开(公告)号：US08632975B2

公开(公告)日：2014-01-21

申请号：US12790760

申请日：2010-05-28

申请人： Peter B. Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett B. Rosenblum ,  Michael Phelan

发明人： Peter B. Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett B. Rosenblum ,  Michael Phelan

IPC分类号： C12Q1/68

CPC分类号： C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

摘要翻译： 本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。 该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。 该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。 标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。 所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

公开(公告)号：US20120322676A1

公开(公告)日：2012-12-20

申请号：US13495748

申请日：2012-06-13

申请人： Yongmei JI ,  Sheung-Mei ("Rita") Shih ,  Lovorka Degoricija ,  Zhaohui Zhou ,  Craig Cummings ,  Manohar Furtado ,  Pius Brzoska ,  Angela Burrell ,  Harrison Leong ,  Xingwang Fang ,  Mangkey Bounpheng ,  Rohan Shah

发明人： Yongmei JI ,  Sheung-Mei ("Rita") Shih ,  Lovorka Degoricija ,  Zhaohui Zhou ,  Craig Cummings ,  Manohar Furtado ,  Pius Brzoska ,  Angela Burrell ,  Harrison Leong ,  Xingwang Fang ,  Mangkey Bounpheng ,  Rohan Shah

IPC分类号： C12Q1/68 ,  C40B30/04 ,  G01N21/76 ,  C07H21/04 ,  G01N27/62

CPC分类号： C12Q1/689 ,  C12R1/01

摘要： Disclosed are genomic sequences for nine strains of Cronobacter spp. (C. sakazakii—696, 701, 680; C. malonaticus—507, 681; C. turicensis—564; C. muytjensii—530; C. dublinensis—582; C. genomosp1—581) and compositions, methods, and kits for detecting, identifying and distinguishing Cronobacter spp. strains from each other and from non-Cronobacter spp. strains. Some embodiments describe isolated nucleic acid compositions unique to certain Cronobacter strains as well as compositions that are specific to all Cronobacter spp. Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of Cronobacter spp. are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR data.

摘要翻译： 公开了九个克罗杆菌属菌株的基因组序列。 （C.sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组sp1-581）和组合物，方法和试剂盒 用于检测，识别和鉴别克罗杆菌属。 菌株彼此和非克罗杆菌属。 菌株。 一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。 还提供引物和探针组合物以及引物和探针的使用方法。 克隆杆菌属鉴定试剂盒 也被描述。 一些实施例涉及用于设置用于PCR数据分析的基于控制的阈值的计算机软件方法。

公开(公告)号：US10428994B2

公开(公告)日：2019-10-01

申请号：US15523564

申请日：2016-01-28

申请人： Zhaohui Zhou

发明人： Zhaohui Zhou

IPC分类号： F16L57/06 ,  B65G53/52 ,  F16L43/00 ,  E04G21/04 ,  F16L9/19

摘要： An auto-fill two-layer two-half wear resistant elbow of a concrete pump truck consists of an outer-layer protection elbow, an inner-layer heterogeneous wear resistant combined elbow and a wear resistant connecting flange. A filling space is provided between the outer-layer protection elbow and the inner-layer heterogeneous wear resistant combined elbow. The wear resistant elbow is designed as a two-layer two-half unique structure including a protection layer and a wear resistant layer. Upon using the wear resistant elbow for the first time, the concrete grout fills the reserved slit via the heterogenous combined elbow and enters the reserved filling space, allowing the outer-layer protection elbow and the inner-layer heterogeneous wear resistant combined elbow to be fixed as a whole.