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公开(公告)号：US06495321B1
公开(公告)日：2002-12-17
申请号：US09445649
申请日：2000-04-06
IPC分类号： C12Q168
CPC分类号： C40B40/02 , C12N15/1027 , C12N15/1037 , C12Q1/6811 , C12Q1/6858 , C12Q2521/319
摘要： The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleofide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.
摘要翻译： 本发明涉及蛋白质功能体外进化的方法。 特别地，该方法涉及从外切核酸酶消化获得的核苷酸片段的改组。 本发明人已经显示衍生自用核酸外切酶消化的亲本多核苷酸序列的多核苷酸片段可以组合以产生编码具有所需特征的多肽的多核苷酸序列。 与母体抗体相比，该方法可有效地应用于具有修饰特征的新抗体或其部分的产生。
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2.发明授权Method for in vitro molecular evolution of protein function using exonuclease and amplification 失效使用外切核酸酶和扩增的体外分子进化蛋白质功能的方法
公开(公告)号：US6159690A
公开(公告)日：2000-12-12
申请号：US98287
申请日：1998-06-16
IPC分类号： C12N15/09 , A61K38/00 , A61K38/43 , A61K39/395 , C12N15/10 , C12P21/02 , C12Q1/68 , C40B40/02 , C12P19/34
CPC分类号： C40B40/02 , C12N15/1027 , C12N15/1037 , C12Q1/6811 , C12Q1/6858 , C12Q2521/319
摘要： The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.
摘要翻译： 本发明涉及蛋白质功能体外进化的方法。 特别地，该方法涉及从外切核酸酶消化获得的核苷酸片段的改组。 本发明人已经显示衍生自用外切核酸酶消化的亲代多核苷酸序列的多核苷酸片段可以组合以产生编码具有所需特征的多肽的多核苷酸序列。 与母体抗体相比，该方法可有效地应用于具有修饰特征的新抗体或其部分的产生。
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3.发明授权Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence 有权蛋白质功能的体外分子进化的方法涉及使用外切核酸酶和两个母体多核苷酸序列的群体公开(公告)号：US07153655B2
公开(公告)日：2006-12-26
申请号：US10321195
申请日：2002-12-17
申请人： Carl Arne Krister Borrebaeck , Ann-Christin Malmborg-Hager , Christina Furebring , Ulf Hans Eskil Soderlind , Rebecka Ingrid Camilla Ottosson
发明人： Carl Arne Krister Borrebaeck , Ann-Christin Malmborg-Hager , Christina Furebring , Ulf Hans Eskil Soderlind , Rebecka Ingrid Camilla Ottosson
CPC分类号： C12N15/1027
摘要： The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new proteins (e.g., antibodies and enzymes) or parts thereof having modified characteristics as compared to the parent protein.
摘要翻译： 本发明涉及蛋白质功能体外进化的方法。 特别地，该方法涉及从外切核酸酶消化获得的核苷酸片段的改组。 本发明人已经显示衍生自用外切核酸酶消化的亲代多核苷酸序列的多核苷酸片段可以组合以产生编码具有所需特征的多肽的多核苷酸序列。 与母体蛋白质相比，该方法可有效地应用于具有修饰特征的新蛋白质（例如抗体和酶）或其部分的产生。
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公开(公告)号：US07432083B2
公开(公告)日：2008-10-07
申请号：US11109264
申请日：2005-04-19
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
摘要： The present invention relates to a method for in vitro creation of molecular libraries evolution of protein function. Particularly, it relates to variability and modification of protein function by shuffling polynucleotide sequence segments. A protein of desired characteristics can be obtained by incorporating variant peptide regions (variant motifs) into defined peptide regions (scaffold sequence). The variant motifs can be obtained from parent DNA which has been subjected to mutagenesis to create a plurality of differently mutated derivatives thereof or they can be obtained from in vivo sequences. These variant motifs can then be incorporated into a scaffold sequence and the resulting coded protein screened for desired characteristics. This method is ideally used for obtaining antibodies with desired characteristics by isolating individual CDR DNA sequences and incorporating them into a scaffold which may, for example, be from a totally different antibody.
摘要翻译： 本发明涉及用于体外产生分子文库蛋白质功能进化的方法。 特别地，其涉及通过混洗多核苷酸序列片段的蛋白质功能的变异性和修饰。 通过将变体肽区域（变体基序）并入限定的肽区域（支架序列）中可获得所需特征的蛋白质。 变体基序可以从已经进行诱变以产生多个不同突变的衍生物的亲本DNA获得，或者可以从体内序列获得。 然后可以将这些变体基序并入支架序列中，并筛选所得到的编码蛋白质以获得所需特征。 该方法理想地用于通过分离各个CDR DNA序列并将其并入到例如来自完全不同的抗体的支架中来获得具有所需特征的抗体。
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公开(公告)号：US06989250B2
公开(公告)日：2006-01-24
申请号：US10118100
申请日：2002-04-08
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
摘要： The present invention relates to a method for in vitro creation of molecular libraries evolution of protein function. Particularly, it relates to variability and modification of protein function by shuffling polynucleotide sequence segments. A protein of desired characteristics can be obtained by incorporating variant peptide regions (variant motifs) into defined peptide regions (scaffold sequence). The variant motifs can be obtained from parent DNA which has been subjected to mutagenesis to create a plurality of differently mutated derivatives thereof or they can be obtained from in vivo sequences. These variant motifs can then be incorporated into a scaffold sequence and the resulting coded protein screened for desired characteristics. This method is ideally used for obtaining antibodies with desired characteristics by isolating individual CDR DNA sequences and incorporating them into a scaffold which may, for example, be from a totally different antibody.
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公开(公告)号：US20080108507A1
公开(公告)日：2008-05-08
申请号：US11735213
申请日：2007-04-13
IPC分类号： C40B10/00 , C07H21/00 , C07H21/04 , C12N15/63 , C12N1/00 , C12P21/00 , C40B40/08 , C07K16/00 , C40B50/06 , C40B30/00
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
摘要： The present invention relates to a method for in vitro creation of molecular libraries evolution of protein function. Particularly, it relates to variability and modification of protein function by shuffling polynucleotide sequence segments. A protein of desired characteristics can be obtained by incorporating variant peptide regions (variant motifs) into defined peptide regions (scaffold sequence). The variant motifs can be obtained from parent DNA which has been subjected to mutagenesis to create a plurality of differently mutated derivatives thereof or they can be obtained from in vivo sequences. These variant motifs can then be incorporated into a scaffold sequence and the resulting coded protein screened for desired characteristics. This method is ideally used for obtaining antibodies with desired characteristics by isolating individual CDR DNA sequences and incorporating them into a scaffold which may, for example, be from a totally different antibody.
摘要翻译： 本发明涉及用于体外产生分子文库蛋白质功能进化的方法。 特别地，其涉及通过混洗多核苷酸序列片段的蛋白质功能的变异性和修饰。 通过将变体肽区域（变体基序）并入限定的肽区域（支架序列）中可获得所需特征的蛋白质。 变体基序可以从已经进行诱变以产生多个不同突变的衍生物的亲本DNA获得，或者可以从体内序列获得。 然后可以将这些变体基序并入支架序列中，并筛选所得到的编码蛋白质以获得所需特征。 该方法理想地用于通过分离各个CDR DNA序列并将其并入到例如来自完全不同的抗体的支架中来获得具有期望特征的抗体。
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公开(公告)号：US20110287486A1
公开(公告)日：2011-11-24
申请号：US13110412
申请日：2011-05-18
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
摘要： The present invention relates to a method for in vitro creation of molecular libraries evolution of protein function. Particularly, it relates to variability and modification of protein function by shuffling polynucleotide sequence segments. A protein of desired characteristics can be obtained by incorporating variant peptide regions (variant motifs) into defined peptide regions (scaffold sequence). The variant motifs can be obtained from parent DNA which has been subjected to mutagenesis to create a plurality of differently mutated derivatives thereof or they can be obtained from in vivo sequences. These variant motifs can then be incorporated into a scaffold sequence and the resulting coded protein screened for desired characteristics. This method is ideally used for obtaining antibodies with desired characteristics by isolating individual CDR DNA sequences and incorporating them into a scaffold which may, for example, be from a totally different antibody.
摘要翻译： 本发明涉及用于体外产生分子文库蛋白质功能进化的方法。 特别地，其涉及通过混洗多核苷酸序列片段的蛋白质功能的变异性和修饰。 通过将变体肽区域（变体基序）并入限定的肽区域（支架序列）中可获得所需特征的蛋白质。 变体基序可以从已经进行诱变以产生多个不同突变的衍生物的亲本DNA获得，或者可以从体内序列获得。 然后可以将这些变体基序并入支架序列中，并筛选所得到的编码蛋白质以获得所需特征。 该方法理想地用于通过分离各个CDR DNA序列并将其并入到例如来自完全不同的抗体的支架中来获得具有期望特征的抗体。
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