公开(公告)号：US06746855B2

公开(公告)日：2004-06-08

申请号：US10337985

申请日：2003-01-08

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P1308

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US20100261233A1

公开(公告)日：2010-10-14

申请号：US12249092

申请日：2008-10-10

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P13/08

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过表达，以及制备L-赖氨酸的方法。

公开(公告)号：US20070054380A1

公开(公告)日：2007-03-08

申请号：US11425091

申请日：2006-06-19

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P13/08 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US20050074791A1

公开(公告)日：2005-04-07

申请号：US10804120

申请日：2004-03-19

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C07K14/34 ,  C12N1/21 ,  C12N15/52 ,  C12P13/08 ,  C12Q1/68

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US06200785B1

公开(公告)日：2001-03-13

申请号：US09353133

申请日：1999-07-14

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfeffere ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfeffere ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P1308

CPC分类号： C07K14/34 ,  C12N15/52 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with amplified lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are amplified and, in particular, overexpressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有扩增的lysE基因（赖氨酸出口载体基因）的棒状杆菌的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被扩增，特别是过表达，以及制备L-赖氨酸的方法。

公开(公告)号：US07094584B2

公开(公告)日：2006-08-22

申请号：US10804120

申请日：2004-03-19

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Möckel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P13/08

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US07981640B2

公开(公告)日：2011-07-19

申请号：US12249092

申请日：2008-10-10

申请人： Caroline Kruetzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Sahm Hermann ,  Miroslav Patek

发明人： Caroline Kruetzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Sahm Hermann ,  Miroslav Patek

IPC分类号： C12P13/08

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and a process for the preparation of L-lysine.

摘要翻译： 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过度表达，以及L-赖氨酸的制备方法。

公开(公告)号：US07435584B2

公开(公告)日：2008-10-14

申请号：US11425091

申请日：2006-06-19

申请人： Caroline Kruetzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Sahm Hermann ,  Miroslav Patek

发明人： Caroline Kruetzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Sahm Hermann ,  Miroslav Patek

IPC分类号： C12N1/21

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US06861246B2

公开(公告)日：2005-03-01

申请号：US09810521

申请日：2001-03-19

申请人： Caroline Kreutzer ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C07K14/34 ,  C12N15/67 ,  C12N15/77 ,  C12P13/08 ,  C12N1/20 ,  C12N9/00 ,  C12N9/12 ,  C12N9/88

CPC分类号： C07K14/34 ,  C12N15/67 ,  C12N15/77

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的pyc基因（丙酮酸羧化酶基因）的棒状杆菌L-赖氨酸生产菌株，其中选自dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因 ），lysE基因（赖氨酸输出载体基因）和dapB基因（二氢吡啶二羧酸还原酶基因），特别是dapA基因，特别是过度表达，以及制备L-赖氨酸的方法。

公开(公告)号：US07585959B2

公开(公告)日：2009-09-08

申请号：US10561906

申请日：2004-06-08

申请人： Miroslav Patek ,  Veronika Elisakova

发明人： Miroslav Patek ,  Veronika Elisakova

IPC分类号： C07H21/04 ,  C07K14/00 ,  C12N15/09

CPC分类号： C12N9/88 ,  C12N9/1022 ,  C12P13/06 ,  C12P13/08

摘要： The present invention provides nucleotide sequences coding for acetohydroxy acid synthetase (AHAS) mutants, the mutated enzymes themselves and a process for the fermentative production of branched-chain amino acids using these enzymes in specific hosts in which genes which code for the modified acetohydroxy acid synthetase (AHAS) are expressed.

摘要翻译： 本发明提供编码乙酰羟酸合成酶（AHAS）突变体的核苷酸序列，突变酶本身以及使用这些酶在特异性宿主中发酵生产支链氨基酸的方法，其中编码修饰的乙酰羟酸合成酶的基因 （AHAS）。