公开(公告)号：US20210389315A1

公开(公告)日：2021-12-16

申请号：US17347892

申请日：2021-06-15

申请人： Case Western Reserve University

发明人： Susann Brady-Kalnay ,  Robert Deissler ,  Robert Brown

IPC分类号： G01N33/543 ,  G01N33/569

摘要： Early stage, rapid, low-cost, and accurate detection of disease components in a biological fluid is critically important. A point of care device can use functionalized magnetic beads to facilitate this detection. The device can include a sample holder with a collection region. A magnet can be used to draw the functionalized nanoparticles bound to the disease component into the collection region, where the disease component is captured. A light source can shine a light beam through the collection region; and a detector can detect the light beam after traversing the collection region to determine whether the disease component is present in the sample.

公开(公告)号：US20210100920A1

公开(公告)日：2021-04-08

申请号：US16608021

申请日：2017-05-24

申请人： CASE WESTERN RESERVE UNIVERSITY

发明人： Susann Brady-Kalnay ,  Mette L. Johansen

IPC分类号： A61K49/10 ,  A61K49/00 ,  A61K49/08 ,  A61K49/14

摘要： An agent for use in detecting, monitoring, and/or imaging cancer cells and/or cancer cell metastasis, migration, dispersal, and/or invasion, and/or for treating cancer in a subject includes a targeting peptide and at least one of a detectable moiety, therapeutic agent, or a theranostic agent that is directly or indirectly linked to the targeting peptide. The targeting peptide specifically binds to and/or complexes with a proteolytically cleaved extracellular fragment of an immunoglobulin (Ig) superfamily cell adhesion molecule that is expressed by a cancer cell or another cell in the cancer cell microenvironment.

公开(公告)号：US20160083734A1

公开(公告)日：2016-03-24

申请号：US14873528

申请日：2015-10-02

申请人： CASE WESTERN RESERVE UNIVERSITY

发明人： Susann Brady-Kalnay

IPC分类号： C12N15/113 ,  A61K47/48 ,  A61K31/7088

摘要： A method of one or more of, inhibiting motility, migration, dispersal, and metastasis of a cell that expresses an RPTP, which is proteolytically cleaved to form intracellular fragments in the cell includes administering to the cell an amount of an agent effective to inhibit one or more of the catalytic activity and function of the proteolytically cleaved intracellular domain-containing fragments of the RPTP.

摘要翻译： 一种或多种抑制表达RPTP的细胞的运动，迁移，分散和转移的方法，所述细胞在蛋白水解切割以形成细胞内的细胞内片段，包括给予细胞一定量的有效抑制一种 或更多的RPTP的蛋白水解裂解的细胞内结构域的片段的催化活性和功能。

公开(公告)号：US20220308048A1

公开(公告)日：2022-09-29

申请号：US17839831

申请日：2022-06-14

申请人： Case Western Reserve University

发明人： Susann Brady-Kalnay ,  Robert Deissler ,  Robert Brown

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/569 ,  G01N21/64 ,  G01N21/11

摘要： Early stage, rapid, low-cost detection of disease components in a biological sample is critically important. A point of care device can include a collection region and can be used to hold a sample that is combined with a fluorescent dye and a plurality of magnetic particles such that disease components in the sample are tagged with the fluorescent dye and the plurality of magnetic particles. At least one magnet can be located next to the collection region to establish a magnetic field gradient to draw the tagged disease components into the collection region from the device. A fluorescence microscope can image the small collection region based on the fluorescent dye to detect the disease components. The fluorescence microscope uses light to excite the fluorescent dye and a filter to transmit light emitted by the fluorescent dye to the fluorescence microscope, while restricting light used to excite the fluorescent dye.

公开(公告)号：US20200041595A1

公开(公告)日：2020-02-06

申请号：US16603335

申请日：2018-04-06

申请人： Case Western Reserve University

发明人： Chris Flask ,  Christian Anderson ,  Xin Yu ,  Nicole Steinmetz ,  Mark A. Griswold ,  Susann Brady-Kalnay

IPC分类号： G01R33/50 ,  A61B5/055 ,  G01R33/56 ,  G06T7/00 ,  G01R33/48

摘要： The present disclosure provides a method of DDCE-MRF. The method can include: a) introducing two or more contrast agents to a region of interest (ROI) of a subject, the two or more contrast agents having different relaxivities; b) measuring a T1 relaxation time and a T2 relaxation time for locations within the ROI using magnetic resonance fingerprinting (MRF); c) determining, using equations that relate the different relaxivities, the T1 relaxation time, the T2 relaxation time, and concentrations of the two or more contrast agents, the concentrations of the two or more contrast agents for each of the locations within the ROI; and d) producing an image depicting the ROI based, at least in part, on the concentrations of the two or more contrast agents.

公开(公告)号：US20140178299A1

公开(公告)日：2014-06-26

申请号：US14188404

申请日：2014-02-24

申请人： Case Western Reserve University

发明人： Susann Brady-Kalnay

IPC分类号： A61K49/00 ,  C07K16/18

CPC分类号： A61K49/0058 ,  A61K49/0032 ,  A61K51/088 ,  C07K16/18 ,  G01N33/57407 ,  G01N2333/70503

摘要： A molecular probe for use in detection of cancer cells expressing an Ig superfamily cell adhesion molecule that binds in a homophilic fashion in a subject includes a targeting agent that specifically binds to and/or complexes with a proteolytically cleaved extracellular fragment of the Ig superfamily cell adhesion molecule.

摘要翻译： 用于检测表达在受试者中以亲和方式结合的Ig超家族细胞粘附分子的癌细胞的分子探针包括与Ig超家族细胞粘附的蛋白水解切割的细胞外片段特异性结合和/或复合的靶向剂 分子。

公开(公告)号：US20200278350A1

公开(公告)日：2020-09-03

申请号：US16805464

申请日：2020-02-28

申请人： CASE WESTERN RESERVE UNIVERSITY

发明人： Susann Brady-Kalnay

IPC分类号： G01N33/574

摘要： A method of determining a prognosis for cancer in a subject and/or a subject with cancer includes measuring a level of proteolytically cleaved extracellular fragments of an immunoglobulin (Ig) superfamily cell adhesion molecule that is expressed by cancer cells obtained from the subject to determine at least one of prognosis for the cancer, the subject's survival, or responsiveness of the cancer to anti-cancer treatment.

公开(公告)号：US10238757B2

公开(公告)日：2019-03-26

申请号：US14188404

申请日：2014-02-24

申请人： Case Western Reserve University

发明人： Susann Brady-Kalnay

IPC分类号： A61K49/00 ,  A61K51/08 ,  G01N33/574 ,  C07K16/18

摘要： A molecular probe for use in detection of cancer cells expressing an Ig superfamily cell adhesion molecule that binds in a homophilic fashion in a subject includes a targeting agent that specifically binds to and/or complexes with a proteolytically cleaved extracellular fragment of the Ig superfamily cell adhesion molecule.

公开(公告)号：US20160349339A1

公开(公告)日：2016-12-01

申请号：US15169166

申请日：2016-05-31

申请人： Case Western Reserve University

发明人： Susann Brady-Kalnay ,  Vikas Gulani ,  Mark Griswold ,  Chris Flask

IPC分类号： G01R33/44 ,  G01R33/563 ,  G01R33/56 ,  G01R33/48 ,  G01R33/485

CPC分类号： G01R33/5614 ,  G01R33/50 ,  G01R33/56509 ,  G01R33/56563

摘要： A system and method is provided for operating a high-field magnetic resonance (MR) system includes performing a series of data acquisition modules without respiratory gating. Each data acquisition module is formed of a steady-state free precession pulse sequence. Performing the series of data acquisition modules includes varying at least one of an amplitude of an excitation pulse or a repetition time of the steady-state free precession pulse sequence between adjacent data acquisition modules in the series of data acquisition modules to acquire a series of MR data with random or pseudo-random imaging acquisition parameters. The series of MR data is compared to a dictionary of signal evolution profiles to determine a match between the series of MR data with at least one signal evolution profile in the dictionary indicating at least one quantitative parameter in the subject.

摘要翻译： 提供用于操作高场磁共振（MR）系统的系统和方法包括执行一系列无呼吸门控的数据采集模块。 每个数据采集模块由稳态自由进动脉冲序列组成。 执行一系列数据采集模块包括改变一系列数据采集模块中的相邻数据采集模块之间的稳态自由进动脉冲序列的激励脉冲的幅度或重复时间中的至少一个，以获得一系列MR 数据随机或伪随机成像采集参数。 将MR数据系列与信号进化曲线的字典进行比较，以确定该系列MR数据与字典中至少一个信号进化曲线之间的匹配，以指示对象中的至少一个定量参数。

公开(公告)号：US09508256B2

公开(公告)日：2016-11-29

申请号：US14068537

申请日：2013-10-31

申请人： Case Western Reserve University

发明人： Susann Brady-Kalnay ,  Vikas Gulani ,  Mark Griswold

IPC分类号： A61K49/06 ,  G01R33/48 ,  G01R33/56 ,  G01R33/563 ,  G01R33/58 ,  A61B5/055 ,  G08C23/06 ,  G01R33/36 ,  A61B5/00

CPC分类号： G08C23/06 ,  A61B5/0017 ,  A61B5/0073 ,  A61B5/055 ,  A61B5/4244 ,  A61K49/06 ,  G01R33/3614 ,  G01R33/3692 ,  G01R33/4822 ,  G01R33/56 ,  G01R33/5608 ,  G01R33/56366 ,  G01R33/58

摘要： Example apparatus and methods concern determining whether a target material appears in a region experiencing nuclear magnetic resonance. One method acquires a baseline value for a magnetic resonance parameter (MRP) while the region is not exposed to a molecular imaging agent that affects the MRP, acquiring a non-specific uptake value for the MRP while the sample is influenced by a non-specific molecular imaging agent and acquiring a specific uptake value for the MRP while the sample is influenced by a specific molecular imaging agent. The non-specific masking problem is solved by characterizing the region as a function of the baseline value, the non-specific uptake value, and the specific uptake value. The function relies on the similarities and differences between non-specific uptake of the non-specific molecular imaging agent, non-specific uptake of the specific molecular imaging agent, and specific uptake of the specific molecular imaging agent.

摘要翻译： 确定目标材料是否出现在经历核磁共振的区域中的示例性设备和方法。 一种方法获得磁共振参数（MRP）的基线值，而该区域不暴露于影响MRP的分子成像剂，获得MRP的非特异性吸收值，而样品受非特异性 分子显像剂，并且在样品受特定分子显像剂影响时获得MRP的特异摄取值。 通过将该区域表征为基准值，非特异性摄取值和特异摄取值的函数来解决非特异性掩蔽问题。 该功能依赖于非特异性分子显像剂的非特异性摄取，特异性分子显像剂的非特异性摄取和特异性分子成像剂的特异性吸收之间的相似性和差异性。