Polypeptide
    5.
    发明申请
    Polypeptide 审中-公开
    多肽

    公开(公告)号:US20070141693A1

    公开(公告)日:2007-06-21

    申请号:US11483220

    申请日:2006-07-07

    IPC分类号: C12N9/32 C12N1/21

    摘要: The invention provides for a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity which may be selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

    摘要翻译: 本发明提供可衍生自具有淀粉酶活性的亲本多肽的PS4变体多肽,其可以选自: 146,157,161,178,179,223,229,272,303,307,309和334; (b)包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; (c)包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和(d)包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参考如SEQ ID NO:1所示的假单胞菌属酵母内切酶序列的位置编号,这种多肽作为食品或饲料添加剂的使用,以及编码其的核酸。