公开(公告)号：US20100189706A1

公开(公告)日：2010-07-29

申请号：US12525303

申请日：2008-01-30

申请人： Cathy Chang ,  Justin T. Stege ,  Nahla Aboushadi ,  Gordana Djordjevic ,  Ellen Burke ,  Peter Luginbuhl ,  Mark Dycaico ,  Toby Richardson ,  John Poland ,  Ying Hefner ,  Stacy Marie Miles

发明人： Cathy Chang ,  Justin T. Stege ,  Nahla Aboushadi ,  Gordana Djordjevic ,  Ellen Burke ,  Peter Luginbuhl ,  Mark Dycaico ,  Toby Richardson ,  John Poland ,  Ying Hefner ,  Stacy Marie Miles

IPC分类号： A61K38/44 ,  A61K38/46 ,  C12N11/16 ,  A23L1/28 ,  C12P21/06 ,  C12P19/34 ,  C12P7/04 ,  C12P7/16 ,  C12P7/06 ,  C12N11/14 ,  C12N11/00 ,  C12N11/08 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/42 ,  C12N5/10 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N15/63 ,  C12P1/00 ,  D21C3/00 ,  C40B40/06 ,  C40B40/10 ,  C11D7/42 ,  C07K16/00 ,  C07H21/04 ,  A01K67/00 ,  A01H5/00

CPC分类号： C12P19/14 ,  C12N9/2437 ,  C12N9/2445 ,  C12P7/04 ,  C12P19/02 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a β-xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts; for example, the invention provides a multi-enzyme system that can hydrolyze polysaccharides in a bagasse component of sugarcane processed in sugar mills. The invention provides enzymes for the bioconversion of lignocellulosic residues into fermentable sugars; and these sugars can be used as a chemical feedstock for the production of ethanol and fuels, including biofuels such as bioethanol, biopropanol, biobutanol and biodiesels.

摘要翻译： 本发明提供具有木质纤维素分解活性的多肽，例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，木糖苷酶（例如，α-氧化酶），阿拉伯呋喃糖苷酶， 和/或葡萄糖氧化酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明提供了可以酶促加工（水解）甘蔗渣，即甘蔗渣降解或用于生物量加工的多肽，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 在一个实施方案中，本发明提供本发明的多肽的耐热性和耐热性形式。 本发明的多肽可用于各种药物，农业和工业环境; 例如，本发明提供了一种可以在糖厂加工的甘蔗的蔗渣组分中水解多糖的多酶体系。 本发明提供用于将木质纤维素残基生物转化成可发酵糖的酶; 并且这些糖可以用作生产乙醇和燃料的化学原料，包括生物燃料如生物乙醇，生物丙醇，生物丁醇和生物柴油。