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    Variant CBH I polypeptides
    3.
    发明授权
    Variant CBH I polypeptides 有权
    变体CBH I多肽

    公开(公告)号：US09234216B2

    公开(公告)日：2016-01-12

    申请号：US13824315

    申请日：2011-10-06

    申请人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    发明人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    IPC分类号： C12P7/14 ,  C12N9/42 ,  C12P19/02 ,  C12P19/14

    CPC分类号： C12P7/14 ,  C12N9/2437 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

    摘要： In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units β-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (β-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like.

    摘要翻译： 在替代实施方案中，本发明提供具有木质纤维素分解（木质纤维素）活性的多肽，例如木质素分解和纤维素分解活性，包括例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶（cbhl）（例如，外切纤维二糖水解酶 例如具有可以逐步释放纤维二糖单元和-1,4葡萄糖 - 葡萄糖二糖的“外显子”活性，β-葡糖苷酶，木聚糖酶，甘露聚糖，木糖苷酶（例如，（β-谷氨酸） 和/或阿拉伯呋喃糖苷酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法在一个实施方案中，本发明提供了本发明的多肽的热稳定性和耐热性形式，使用本发明的多肽和核酸 在各种制药，农业和工业环境中;例如，作为生物质生物转化的酶，例如木质纤维素残基，i 在一方面，这些糖用作生产乙醇和燃料的化学原料，例如生物燃料，例如合成液体或气体燃料，包括乙醇，甲醇等。

    VARIANT CBH I POLYPEPTIDES
    5.
    发明申请
    VARIANT CBH I POLYPEPTIDES 有权
    变种CBH多糖

    公开(公告)号：US20130203128A1

    公开(公告)日：2013-08-08

    申请号：US13824315

    申请日：2011-10-06

    申请人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    发明人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    IPC分类号： C12N9/42 ,  C12P19/02

    CPC分类号： C12P7/14 ,  C12N9/2437 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

    摘要： In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units β-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (β-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like.

    摘要翻译： 在替代实施方案中，本发明提供具有木质纤维素分解（木质纤维素）活性的多肽，例如木质素分解和纤维素分解活性，包括例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶（cbhl）（例如，外切纤维二糖水解酶 例如，具有可以逐步释放纤维二糖单位β-1,4葡萄糖 - 葡萄糖二糖的“外部”活性），β-葡糖苷酶，木聚糖酶，甘露聚糖，木糖苷酶（例如（β-木糖苷酶）和/ 或阿拉伯呋喃糖苷酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法在一个实施方案中，本发明提供了本发明的多肽的耐热和耐热形式。本发明的多肽和核酸用于 各种制药，农业和工业环境;例如，作为生物质生物转化的酶，例如木质纤维素残基，转化为Fe 可再生糖，其中一方面，这些糖被用作用于生产乙醇和燃料的化学原料，例如生物燃料，例如合成液体或气体燃料，包括乙醇，甲醇等。

    Proteases, nucleic acids encoding them and methods for making and using them
    6.
    发明授权
    Proteases, nucleic acids encoding them and methods for making and using them 有权
    蛋白酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US07642079B2

    公开(公告)日：2010-01-05

    申请号：US10530643

    申请日：2003-10-10

    申请人： Michelle Cayouette ,  Connie Jo Hansen ,  Amy McClure ,  May Sun ,  Svetlana Gramatikova ,  Mark Dycaico ,  Nelson R. Barton ,  Justin T. Stege ,  Nahla M. Aboushadi

    发明人： Michelle Cayouette ,  Connie Jo Hansen ,  Amy McClure ,  May Sun ,  Svetlana Gramatikova ,  Mark Dycaico ,  Nelson R. Barton ,  Justin T. Stege ,  Nahla M. Aboushadi

    IPC分类号： C12N9/48

    CPC分类号： C12N9/52 ,  A01K2217/05

    摘要： The invention is directed to polypeptides having protease activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of diagnostic, therapeutic, and industrial contexts. The polypeptides of the invention can be used as, e.g., an additive for a detergent, for processing foods and for chemical synthesis utilizing a reverse reaction. Additionally, the polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to ethanol diodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in increasing starch yield from corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.

    摘要翻译： 本发明涉及具有蛋白酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明的多肽可用于多种诊断，治疗和工业背景。 本发明的多肽可用作例如用于洗涤剂的添加剂，用于加工食品和利用反向反应进行化学合成。 此外，本发明的多肽可用于食品加工，酿造，浴添加剂，醇生产，肽合成，对映选择性，皮革工业中的皮革制备，废物管理和动物降解，摄影工业中的银回收，医疗， 丝绸脱胶，生物膜降解，生物质转化为乙醇二聚体，抗微生物剂和消毒剂，个人护理和化妆品，生物技术试剂，用于增加玉米湿法研磨的淀粉产量，以及药物如消化助剂和抗炎（抗炎）剂。

    Polypeptides Having Endoglucanase Activity
    8.
    发明申请
    Polypeptides Having Endoglucanase Activity 审中-公开
    具有内切葡聚糖酶活性的多肽

    公开(公告)号：US20130244292A1

    公开(公告)日：2013-09-19

    申请号：US13840091

    申请日：2013-03-15

    申请人： John Poland ,  Justin T. Stege

    发明人： John Poland ,  Justin T. Stege

    IPC分类号： C12N9/24

    CPC分类号： C12P19/14 ,  C12N9/2437 ,  C12N9/244 ,  C12P19/02

    摘要： The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant.

    摘要翻译： 本发明涉及具有内切葡聚糖酶活性的家族5糖苷水解酶变体，编码家族5糖苷水解酶变体的多核苷酸，载体，包含多核苷酸的宿主细胞，以及使用5族糖苷水解酶变体的方法。