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    Methods of Nucleic acid amplification and sequencing
    1.
    发明申请
    Methods of Nucleic acid amplification and sequencing 有权
    核酸扩增和测序方法

    公开(公告)号：US20140228254A1

    公开(公告)日：2014-08-14

    申请号：US14150607

    申请日：2014-01-08

    申请人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jaques Mermod ,  Gerardo Turcatti

    发明人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jaques Mermod ,  Gerardo Turcatti

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1068 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/543

    摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

    摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，其包含待扩增或测序的核酸，其中所述核酸在5 '结束寡核苷酸序列Y，在3'端有寡核苷酸序列Z，另外核酸在5'端带有用于将核酸连接到固体支持物上的方法; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。

    Methods of nucleic acid amplification and sequencing
    2.
    发明申请
    Methods of nucleic acid amplification and sequencing 有权
    核酸扩增和测序方法

    公开(公告)号：US20080160580A1

    公开(公告)日：2008-07-03

    申请号：US11506146

    申请日：2006-08-17

    申请人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jacques Mermod ,  Gerardo Turcatti

    发明人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jacques Mermod ,  Gerardo Turcatti

    IPC分类号： C12P19/34

    CPC分类号： C12N15/1068 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/543

    摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

    摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，其包含待扩增或测序的核酸，其中所述核酸包含在5 '结束寡核苷酸序列Y，在3'端有寡核苷酸序列Z，另外核酸在5'端带有用于将核酸连接到固体支持物上的方法; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。

    Methods of nucleic acid amplification and sequencing
    3.
    发明授权
    Methods of nucleic acid amplification and sequencing 有权
    核酸扩增和测序方法

    公开(公告)号：US08652810B2

    公开(公告)日：2014-02-18

    申请号：US11506146

    申请日：2006-08-17

    申请人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jacques Mermod ,  Gerardo Turcatti

    发明人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jacques Mermod ,  Gerardo Turcatti

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12M1/34 ,  C12M3/00 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12N15/1068 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/543

    摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

    摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，其包含待扩增或测序的核酸，其中所述核酸在5 '结束寡核苷酸序列Y，在3'端有寡核苷酸序列Z，另外核酸在5'端带有用于将核酸连接到固体支持物上的方法; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。

    Methods of nucleic acid amplification and sequencing
    4.
    发明授权
    Methods of nucleic acid amplification and sequencing 有权
    核酸扩增和测序方法

    公开(公告)号：US07115400B1

    公开(公告)日：2006-10-03

    申请号：US09806531

    申请日：1999-09-30

    申请人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jacques Mermod ,  Gerardo Turcatti

    发明人： Celine Adessi ,  Eric Kawashima ,  Pascal Mayer ,  Jean-Jacques Mermod ,  Gerardo Turcatti

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12M1/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1068 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/543

    摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

    摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，所述核酸模板包含待扩增或测序的核酸，其中所述待扩增的核酸或 其中所述核酸在5'末端含有寡核苷酸序列Y，在3'端含有寡核苷酸序列Z，另外核酸在5'末端携带一个用于附着的装置 所述核酸至固体支持物; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。