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公开(公告)号：US20080160580A1
公开(公告)日：2008-07-03
申请号：US11506146
申请日：2006-08-17
IPC分类号： C12P19/34
CPC分类号： C12N15/1068 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2565/543
摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.
摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，其包含待扩增或测序的核酸，其中所述核酸包含在5 '结束寡核苷酸序列Y，在3'端有寡核苷酸序列Z，另外核酸在5'端带有用于将核酸连接到固体支持物上的方法; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。
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公开(公告)号：US08652810B2
公开(公告)日：2014-02-18
申请号：US11506146
申请日：2006-08-17
CPC分类号： C12N15/1068 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2565/543
摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.
摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，其包含待扩增或测序的核酸，其中所述核酸在5 '结束寡核苷酸序列Y，在3'端有寡核苷酸序列Z，另外核酸在5'端带有用于将核酸连接到固体支持物上的方法; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。
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公开(公告)号：US07115400B1
公开(公告)日：2006-10-03
申请号：US09806531
申请日：1999-09-30
CPC分类号： C12N15/1068 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2565/543
摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.
摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，所述核酸模板包含待扩增或测序的核酸，其中所述待扩增的核酸或 其中所述核酸在5'末端含有寡核苷酸序列Y，在3'端含有寡核苷酸序列Z，另外核酸在5'末端携带一个用于附着的装置 所述核酸至固体支持物; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。
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公开(公告)号：US20140228254A1
公开(公告)日：2014-08-14
申请号：US14150607
申请日：2014-01-08
IPC分类号： C12N15/10
CPC分类号： C12N15/1068 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2565/543
摘要： Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5′ end an oligonucleotide sequence Y and at the 3′ end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5′ end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5′ end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5′ ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.
摘要翻译： 用于扩增和测序至少一种核酸的方法，包括以下步骤：（1）形成至少一个核酸模板，其包含待扩增或测序的核酸，其中所述核酸在5 '结束寡核苷酸序列Y，在3'端有寡核苷酸序列Z，另外核酸在5'端带有用于将核酸连接到固体支持物上的方法; （2）将所述核酸模板与一个或多个菌落引物X混合，其可与寡核苷酸序列Z杂交，并在5'末端携带用于将菌落引物连接至固体支持物的手段，在存在 固体支持物，使得核酸模板和集落引物的5'末端与固体支持物结合; （3）对结合的模板进行一个或多个核酸扩增反应，从而产生核酸集落，并且任选地，进行生成的一个或多个核酸集落的至少一个序列测定步骤。 用于这些方法的固体支撑物，试剂盒和仪器。
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公开(公告)号：US20050100900A1
公开(公告)日：2005-05-12
申请号：US10449010
申请日：2003-06-02
申请人： Eric Kawashima , Laurent Farinelli , Pascal Mayer
发明人： Eric Kawashima , Laurent Farinelli , Pascal Mayer
CPC分类号： C12Q1/6837 , C12Q1/6834 , C12Q1/6869 , C12Q1/6874 , C12Q2565/543 , C12Q2565/518 , C12Q2565/537 , C12Q2535/101
摘要： A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provided further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.
摘要翻译： 核酸分子可以与合适的固定化引物退火。 然后可以延伸引物，并且分子和引物可以彼此分离。 然后将延伸的引物退火至另一个固定的引物，另一个引物可以延伸。 然后可以将两种延伸的引物彼此分离，并可用于提供进一步的延伸引物。 可以重复该过程以提供扩增的，固定化的核酸分子。 这些可用于许多不同的目的，包括测序，筛选，诊断，原位核酸合成，监测基因表达，核酸指纹图谱等。
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公开(公告)号：US6133434A
公开(公告)日：2000-10-17
申请号：US842079
申请日：1997-04-28
IPC分类号： C07K14/705 , C07H21/00 , C07K14/475
CPC分类号： C07K14/705
摘要： The present invention relates, in general, to a purinergic receptor and, in particular, to a P2X.sub.7 (also designated P2Z) receptor. The invention further relates to a nucleic acid encoding the P2X.sub.7 receptor and to a method of producing P2X.sub.7 recombinantly using same. The invention also relates to a method of screening compounds for their ability to inhibit P2X.sub.7 activity and thereby for their usefulness in treating a variety of diseases/disorders, including arthritic and respiratory disorders and neurodegenerative diseases.
摘要翻译： 本发明一般涉及嘌呤能受体，特别涉及P2X7（也称为P2Z）受体。 本发明还涉及编码P2X7受体的核酸和使用其重组的P2X7的生产方法。 本发明还涉及一种筛选化合物抑制P2X7活性的能力的方法，从而其可用于治疗各种疾病/病症，包括关节炎和呼吸障碍和神经变性疾病。
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7.发明授权公开(公告)号：US06509163B1
公开(公告)日：2003-01-21
申请号：US09638857
申请日：2000-08-15
IPC分类号： G01N3353
CPC分类号： C07K14/705
摘要： The present invention relates, in general, to a purinergic receptor and, particular, to a P2X7 (also designated P2Z) receptor. The invention further relates to a nucleic acid encoding the P2X7 receptor and to a method of producing P2X7 combinantly using same. The invention also relates to a method of screening compounds for their ability to inhibit P2X7 activity and thereby for their usefulness in treating a variety of diseases/disorders, including arthritic and respiratory disorders and neurodegenerative diseases.
摘要翻译： 本发明一般涉及嘌呤能受体，特别涉及P2X7（也称为P2Z）受体。 本发明还涉及编码P2X7受体的核酸和使用其组合生产P2X7的方法。 本发明还涉及一种筛选化合物抑制P2X7活性的能力的方法，从而其可用于治疗各种疾病/病症，包括关节炎和呼吸障碍和神经变性疾病。
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