公开(公告)号：US07951334B2

公开(公告)日：2011-05-31

申请号：US12044738

申请日：2008-03-07

申请人： Chad A. Mirkin ,  Linette Demers ,  David S. Ginger

发明人： Chad A. Mirkin ,  Linette Demers ,  David S. Ginger

IPC分类号： B01L3/02

CPC分类号： G03F7/0002 ,  G01Q80/00 ,  Y10T436/2575

摘要： The use of direct-write nanolithography to generate anchored, nanoscale patterns of nucleic acid on different substrates is described, including electrically conductive and insulating substrates. Modification of nucleic acid, including oligonucleotides, with reactive groups such as thiol groups provides for patterning with use of appropriate scanning probe microscopic tips under appropriate conditions. The reactive groups provide for chemisorption or covalent bonding to the substrate surface. The resulting nucleic acid features, which exhibit good stability, can be hybridized with complementary nucleic acids and probed accordingly with use of, for example, nanoparticles functionalized with nucleic acids. Patterning can be controlled by selection of tip treatment, relative humidity, and nucleic acid structure.

摘要翻译： 描述了使用直写式纳米光刻技术在不同基底上产生锚定的纳米级核酸图案，包括导电和绝缘基底。 含有反应性基团如硫醇基团的核酸（包括寡核苷酸）的修饰使得在合适的条件下使用合适的扫描探针显微镜尖端进行图案化。 反应性基团提供化学吸附或共价结合到基底表面。 所得到的表现出良好稳定性的核酸特征可与互补核酸杂交，并使用例如用核酸官能化的纳米颗粒进行相应的探测。 可以通过选择尖端处理，相对湿度和核酸结构来控制图案化。

公开(公告)号：US07361310B1

公开(公告)日：2008-04-22

申请号：US10307515

申请日：2002-12-02

申请人： Chad A. Mirkin ,  Linette Demers ,  David S. Ginger

发明人： Chad A. Mirkin ,  Linette Demers ,  David S. Ginger

IPC分类号： B01L3/02

CPC分类号： G03F7/0002 ,  G01Q80/00 ,  Y10T436/2575

摘要： The use of direct-write nanolithography to generate anchored, nanoscale patterns of nucleic acid on different substrates is described, including electrically conductive and insulating substrates. Modification of nucleic acid, including oligonucleotides, with reactive groups such as thiol groups provides for patterning with use of appropriate scanning probe microscopic tips under appropriate conditions. The reactive groups provide for chemisorption or covalent bonding to the substrate surface. The resulting nucleic acid features, which exhibit good stability, can be hybridized with complementary nucleic acids and probed accordingly with use of, for example, nanoparticles functionalized with nucleic acids. Patterning can be controlled by selection of tip treatment, relative humidity, and nucleic acid structure.

摘要翻译： 描述了使用直写式纳米光刻技术在不同基底上产生锚定的纳米级核酸图案，包括导电和绝缘基底。 含有反应性基团如硫醇基团的核酸（包括寡核苷酸）的修饰使得在合适的条件下使用合适的扫描探针显微镜尖端进行图案化。 反应性基团提供化学吸附或共价结合到基底表面。 所得到的表现出良好稳定性的核酸特征可与互补核酸杂交，并使用例如用核酸官能化的纳米颗粒进行相应的探测。 可以通过选择尖端处理，相对湿度和核酸结构来控制图案化。

公开(公告)号：US07842344B2

公开(公告)日：2010-11-30

申请号：US10788414

申请日：2004-03-01

申请人： Chad A. Mirkin ,  Jung-Hyurk Lim ,  David S. Ginger ,  Jwa-Min Nam ,  Ki-Bum Lee ,  Linette Demers ,  Albena Ivanisevic

发明人： Chad A. Mirkin ,  Jung-Hyurk Lim ,  David S. Ginger ,  Jwa-Min Nam ,  Ki-Bum Lee ,  Linette Demers ,  Albena Ivanisevic

IPC分类号： B05D1/36

CPC分类号： G03B27/42 ,  Y10S977/857 ,  Y10S977/884 ,  Y10S977/885 ,  Y10S977/886

摘要： The present invention relates to the use of direct-write lithographic printing of proteins and peptides onto surfaces. In particular, the present invention relates to methods for creating protein and peptide arrays and compositions derived therefrom. Nanoscopic tips can be used to deposit the peptide or protein onto the surface to produce a pattern. The pattern can be dots or lines having dot diameter and line width of less than 1,000 nm. The tips and the substrate surfaces can be adapted for the peptide and protein lithography.

摘要翻译： 本发明涉及将蛋白质和肽直接写入平版印刷在表面上的用途。 特别地，本发明涉及用于产生蛋白质和肽阵列的方法和由其衍生的组合物。 可以使用纳米尖端将肽或蛋白质沉积到表面上以产生图案。 图案可以是点直径和线宽小于1,000nm的点或线。 尖端和底物表面可适用于肽和蛋白质光刻。

公开(公告)号：US20080269073A1

公开(公告)日：2008-10-30

申请号：US12044738

申请日：2008-03-07

申请人： Chad A. Mirkin ,  Linette Demers ,  David S. Ginger

发明人： Chad A. Mirkin ,  Linette Demers ,  David S. Ginger

IPC分类号： C40B40/06 ,  C40B50/18

CPC分类号： G03F7/0002 ,  G01Q80/00 ,  Y10T436/2575

摘要： The use of direct-write nanolithography to generate anchored, nanoscale patterns of nucleic acid on different substrates is described, including electrically conductive and insulating substrates. Modification of nucleic acid, including oligonucleotides, with reactive groups such as thiol groups provides for patterning with use of appropriate scanning probe microscopic tips under appropriate conditions. The reactive groups provide for chemisorption or covalent bonding to the substrate surface. The resulting nucleic acid features, which exhibit good stability, can be hybridized with complementary nucleic acids and probed accordingly with use of, for example, nanoparticles functionalized with nucleic acids. Patterning can be controlled by selection of tip treatment, relative humidity, and nucleic acid structure.

摘要翻译： 描述了使用直写式纳米光刻技术在不同基底上产生锚定的纳米级核酸图案，包括导电和绝缘基底。 含有反应性基团如硫醇基团的核酸（包括寡核苷酸）的修饰使得在合适的条件下使用合适的扫描探针显微镜尖端进行图案化。 反应性基团提供化学吸附或共价结合到基底表面。 所得到的表现出良好稳定性的核酸特征可与互补核酸杂交，并使用例如用核酸官能化的纳米颗粒进行相应的探测。 可以通过选择尖端处理，相对湿度和核酸结构来控制图案化。