摘要:
Provided is a new type serum-free medium. The medium comprises: DMEM with high glucose (the content of glucose being 4.5 g/L), B27, recombinant human basic fibrolast growth factor (b-FGF), nicotinamide, N-2, vinblastine III (conophylline), non-essential amino acid (NEAA), heparin, epidermal growth factor (EGF), hepatocyte growth factor (HGF), a serum replacement (SR), an insulin-transferrin-selenium complex (ITS), and pentagastrin. Inducing differentiation of mesenchymal stem cells into insulin-secretion-like cells can be achieved in six days in one step using the medium.
摘要:
A compound additive having a biological activation function. The compound additive contains water or phosphate buffer, and multiple proteins and various factors dissolved therein. The compound additive can be added into a culture medium for cell cultivation, and can also be directly used or added into a skin repair product or a cosmetic product so as to achieve certain skin repair and cosmetic effects.
摘要:
Provided is a serum-free culture medium, the ingredients of the culture medium comprising 0.05-0.2 parts by volume of β-mercaptoethanol, 0.5-2 parts by volume of non-essential amino acid aqueous solution, 4-6 parts by volume of human mesenchymal stem cell culture supernatant concentrate, and 90-95 parts by volume of a-MEM/DMEM-F12 and recombinant human alkaline fibroblast growth factor of a final concentration of 5-5 ng/ml. The present culture medium is used for carrying out stem cell culture.
摘要:
Provided is a method for separating and extracting mesenchymal stem cells from the human umbilical cord. The method uses healthy neonatal umbilical cord tissue; after cleaning and disinfection, mechanically pulverising same, separating the Wharton's jelly, and after treating with erythrocyte lysate, carrying out suspension culture in a serum-free culture medium. Replacing the liquid every 3-5 days; after the plate adherence rate reaches 30-70%, carrying out trypsin digestion, and then collecting the cells by centrifugation for passage amplification, until the rate of confluence of the cells reaches 80-90% confluence, thereby obtaining high purity umbilical cord mesenchymal stem cells.
摘要:
A serum-free culture method for human umbilical cord mesenchymal stem cells (hUC-MSC), said method using a step-by-step method to culture hUC-MSC: first using a TME culture medium for culturing for 3-4 hours to promote hUC-MSC adherence, and then switching to a TMD culture medium for rapid amplification.
摘要:
A 3D time series vector sediment trap includes a base disposed with trap pipes which includes water flow pipes and sedimentation pipes. The water flow pipes have a horizontal water inlet at the front end, a vertical downward water outlet at the back end and the water flow pipe is internally provided with a filter screen which tilts towards the water inlet. The filter screen is internally tangential to the water flow pipe, and the sedimentation pipes are vertically fixed underneath the water flow pipes. The top end of the sedimentation pipes have an opening and bottom end thereof is closed. The opening is connected to the water flow pipe and directly faces the filter screen so that materials with a diameter greater than the screen pore diameter are intercepted by the filter screen in the water flow pipe and collected in the sedimentation pipe.
摘要:
A smart 3GDT schema has been disclosed, in detail, a method for controlling the communication of a network system has been disclosed. The network system comprises a UE, a NodeB, a RNC, a SGSN, and a GW. The UE is arranged to be in communication with the RNC via the NodeB, and the RNC is arranged to be in communication with the SGSN which in turn being arranged to be in communication with the GW for non-3GDT communication of the network system, or the RNC is arranged to be in communication with the GW for 3GDT communication of the network system. In this method, statistics of payload transferred between the UE and the GW has been monitored, and if the statistics of payload within a predetermined time period exceeds a first threshold, and the UE is in the non-3GDT communication, then switching the non-3GDT communication to the 3GDT communication. The present application has also disclosed the SGSN, GW, and the network system adaptive to perform the method.
摘要:
A plurality of web documents that include indicators corresponding to one or more selectable like objects may be obtained. A corresponding web domain associated with each of the plurality of obtained web documents may be determined. A domain total like object count of the indicators corresponding to the one or more selectable like objects may be determined for each one of the obtained plurality of web documents, for each determined corresponding web domain. A candidate group of the corresponding web domains may be determined based on a comparison of a predetermined first threshold value with one or more of the domain total like object counts. A likefarm domain confidence score may be determined for each one of a second group of the corresponding web domains based on a decision tree function that is based on a plurality of domain attributes.
摘要:
The present invention discloses a Softswitch and system for implementing the intelligent network in the next generation networks and a method for interconnecting the intelligent networks to the PSTN. The Softswitch comprises: a network adaptive device for implementing the communication between the Softswitch and other devices in said network, as well as receiving the call request; a call server for determining whether the call received by said network adaptive device is a common call or the call of the intelligent network and processing the common call; and an INAP adapter for responding the call of the intelligent network and encoding/decoding the INAP message.