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    Genes encoding nematode toxins
    1.
    发明授权
    Genes encoding nematode toxins 有权
    编码线虫毒素的基因

    公开(公告)号：US08404934B2

    公开(公告)日：2013-03-26

    申请号：US12638591

    申请日：2009-12-15

    申请人： Cheryl L. Peters ,  Brian Vande Berg ,  Brian Carr ,  Julia T. Daum ,  Vadim Beilinson ,  Sandra Volrath ,  Candace Poutre ,  Kimberly Sampson

    发明人： Cheryl L. Peters ,  Brian Vande Berg ,  Brian Carr ,  Julia T. Daum ,  Vadim Beilinson ,  Sandra Volrath ,  Candace Poutre ,  Kimberly Sampson

    IPC分类号： C12N1/21 ,  C12N15/32 ,  C12N15/82 ,  A01N37/18 ,  C07K14/325

    CPC分类号： C12N15/8241 ,  C12N9/0059 ,  C12N9/0071 ,  C12N15/8285 ,  C12Y110/03001 ,  C12Y114/18001 ,  Y02A40/164

    摘要： Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.

    摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀线虫活性的组合物和方法。 提供了包含杀线虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀线虫剂核酸分子。 另外，包含对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包括编码SEQ ID NO：4,5,8,9,13,14,77,48或49所示氨基酸序列的核苷酸序列，所列出的核苷酸序列 在SEQ ID NO：1,2,3,6,7,10,11，12，15，45或46中，以及其变体和片段。

    Plants with Improved Nitrogen Utilization and Stress Tolerance
    5.
    发明申请
    Plants with Improved Nitrogen Utilization and Stress Tolerance 审中-公开
    具有改善氮利用和胁迫耐受性的植物

    公开(公告)号：US20120144520A1

    公开(公告)日：2012-06-07

    申请号：US13309308

    申请日：2011-12-01

    申请人： James McLaren ,  Nicholas Duck ,  Brian Vande Berg ,  Alissa Schawalder ,  Vadim Beilinson ,  Jill Hinson

    发明人： James McLaren ,  Nicholas Duck ,  Brian Vande Berg ,  Alissa Schawalder ,  Vadim Beilinson ,  Jill Hinson

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N1/21 ,  C12N15/82 ,  A01H5/10

    CPC分类号： C12N15/8271 ,  C12N15/8216 ,  C12N15/8261 ,  Y02A40/146

    摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.

    摘要翻译： 本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。 在各种实施方案中，载体构建体包含选自SEQ ID NO： 34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

    Plants with improved nitrogen utilization and stress tolerance
    6.
    发明申请
    Plants with improved nitrogen utilization and stress tolerance 审中-公开
    具有改善氮利用和胁迫耐受性的植物

    公开(公告)号：US20100162433A1

    公开(公告)日：2010-06-24

    申请号：US11977768

    申请日：2007-10-26

    申请人： James McLaren ,  Nicholas Duck ,  Brian Vande Berg ,  Alissa Schawalder ,  Vadim Beilinson ,  Jill Hinson

    发明人： James McLaren ,  Nicholas Duck ,  Brian Vande Berg ,  Alissa Schawalder ,  Vadim Beilinson ,  Jill Hinson

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/74 ,  C07K16/00 ,  C12N1/21 ,  C12N5/10

    CPC分类号： C12N15/8271 ,  C12N15/8216 ,  C12N15/8261 ,  Y02A40/146

    摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct includes one or more nucleic acid sequences selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.

    摘要翻译： 本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。 在各种实施方案中，载体构建体包含一个或多个选自SEQ ID NO： 32,34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

    Synthetic AXMI-004 delta-endotoxin genes and methods for their use
    7.
    发明授权
    Synthetic AXMI-004 delta-endotoxin genes and methods for their use 有权
    合成AXMI-004δ-内毒素基因及其使用方法

    公开(公告)号：US08541366B2

    公开(公告)日：2013-09-24

    申请号：US12209354

    申请日：2008-09-12

    申请人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso ,  Vadim Beilinson ,  Theodore Kahn

    发明人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso ,  Vadim Beilinson ,  Theodore Kahn

    IPC分类号： A01N37/18 ,  C07K14/325

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided, particularly synthetically-derived coding sequences. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, or 18, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 6, 7, 8, 10, 12, 14, 16, or 17, as well as variants and fragments thereof.

    摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供包含δ-内毒素多肽的编码序列的组合物，特别是合成来源的编码序列。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：9,11,13,15或18所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2，4，6，7，8，10，12，14，16或17，以及其变体和片段。

    Chlamydomonas EPSPS chloroplast transit peptide (CTP) and expression cassettes and transgenic plants utilizing the CTP
    8.
    发明授权
    Chlamydomonas EPSPS chloroplast transit peptide (CTP) and expression cassettes and transgenic plants utilizing the CTP 有权
    衣原体EPSPS叶绿体转运肽（CTP）和表达盒和利用CTP的转基因植物

    公开(公告)号：US08426204B2

    公开(公告)日：2013-04-23

    申请号：US12555175

    申请日：2009-09-08

    申请人： Philip E. Hammer ,  Vadim Beilinson ,  Todd K. Hinson

    发明人： Philip E. Hammer ,  Vadim Beilinson ,  Todd K. Hinson

    IPC分类号： A01H5/00 ,  C12N5/04 ,  C12N15/82

    CPC分类号： C12N15/8279 ,  C12N15/8214 ,  C12N15/8274

    摘要： The present invention provides compositions and methods for targeting polypeptides to the chloroplasts of higher plants. Compositions include expression cassettes having a nucleotide sequence encoding a chloroplast targeting peptide (CTP) operably linked to a nucleotide sequence of interest, wherein the CTP is derived from Chlamydomonas sp. Plant transformation vectors, plants and plant cells having the CTP sequences are also encompassed, as well as variants and fragments of the CTP sequences. Methods for expressing a heterologous nucleotide sequence in a plant using the CTP sequences disclosed herein are also provided.

    摘要翻译： 本发明提供了将多肽靶向高等植物的叶绿体的组合物和方法。 组合物包括具有编码与感兴趣的核苷酸序列可操作地连接的叶绿体靶向肽（CTP）的核苷酸序列的表达盒，其中CTP衍生自衣藻属。 还包括具有CTP序列的植物转化载体，植物和植物细胞，以及CTP序列的变体和片段。 还提供了使用本文公开的CTP序列在植物中表达异源核苷酸序列的方法。

    Bacterial Glutamine Synthetases and Methods of Use
    9.
    发明申请
    Bacterial Glutamine Synthetases and Methods of Use 有权
    细菌谷氨酰胺合成酶和使用方法

    公开(公告)号：US20110136200A1

    公开(公告)日：2011-06-09

    申请号：US13027666

    申请日：2011-02-15

    申请人： Nicholas B. Duck ,  Todd K. Hinson ,  Vadim Beilinson ,  Laura Cooper Schouten ,  Daniel John Tomso

    发明人： Nicholas B. Duck ,  Todd K. Hinson ,  Vadim Beilinson ,  Laura Cooper Schouten ,  Daniel John Tomso

    IPC分类号： C12N9/00

    CPC分类号： C12N15/8277 ,  C12N9/93

    摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.

    摘要翻译： 提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。 提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。 另外，包括对应于多核苷酸的多肽。 特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

    Bacterial glutamine synthetases and methods of use
    10.
    发明授权
    Bacterial glutamine synthetases and methods of use 有权
    细菌性谷氨酰胺合成酶及其使用方法

    公开(公告)号：US08101389B2

    公开(公告)日：2012-01-24

    申请号：US13027666

    申请日：2011-02-15

    申请人： Nicholas B. Duck ,  Todd K. Hinson ,  Vadim Beilinson ,  Laura Cooper Schouten ,  Daniel John Tomso

    发明人： Nicholas B. Duck ,  Todd K. Hinson ,  Vadim Beilinson ,  Laura Cooper Schouten ,  Daniel John Tomso

    IPC分类号： C12N9/00

    CPC分类号： C12N15/8277 ,  C12N9/93

    摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.

    摘要翻译： 提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。 提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。 另外，包括对应于多核苷酸的多肽。 特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。