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公开(公告)号：US5059538A
公开(公告)日：1991-10-22
申请号：US896923
申请日：1986-08-15
申请人： Chikateru Nozaki , Hiroshi Nakatake , Yoichiro Kino , Tatsuo Eto , Keiichi Makizumi , Nobuya Ohtomo
发明人： Chikateru Nozaki , Hiroshi Nakatake , Yoichiro Kino , Tatsuo Eto , Keiichi Makizumi , Nobuya Ohtomo
IPC分类号： C12N15/09 , A61K39/00 , C07H21/04 , C07K14/035 , C12N15/00 , C12N15/38 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/865
CPC分类号： C12N15/81 , C07K14/005 , A61K39/00 , C12N2710/16622 , Y10S435/942
摘要： A novel recombinant plasmid inserted with a herpes simplex virus gene, which comprises a plasmid vector containing a yeast DNA sequence and an Escherichia coli DNA sequence and carrying a promoter region and a herpes simplex virus gN gene (HSVgB) gene) recombined thereto under control of the promoter, said HSVgB gene lacking an N-terminal portion of the gene including a signal sequence-encoding region and optionally further lacking the region downstream therefrom, such as a gB gene lacking a DNA sequence encoding the N-terminal 30 amino acids, and a gB gene lacking a DNA sequence encoding the N-terminal 83 amino acids. The recombinant plasmid is useful for the production of transformed yeast, which is useful for the production of HSVgB proteins suitable for producing HSV vaccine and diagnostic reagents for herpes simplex virus infections.
摘要翻译： 一种插入单纯疱疹病毒基因的新型重组质粒，其包含含有酵母DNA序列和大肠杆菌DNA序列并携带启动子区域和单纯疱疹病毒gN基因（HSVgB）基因）的质粒载体， 启动子，所述HSVgB基因缺失包含信号序列编码区的基因的N末端部分，并且任选地进一步缺少其下游区域，例如缺乏编码N末端30个氨基酸的DNA序列的gB基因，以及 缺乏编码N-末端83个氨基酸的DNA序列的gB基因。 重组质粒可用于生产转化酵母，其可用于生产适用于生产HSV疫苗的HSVgB蛋白和用于单纯疱疹病毒感染的诊断试剂。
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2.发明授权Cell usable in serum-free culture and suspension culture and process for producing virus for vaccine by using the cell 失效可用于无血清培养和悬浮培养的细胞以及通过使用细胞产生疫苗病毒的方法公开(公告)号：US06825036B2
公开(公告)日：2004-11-30
申请号：US10220434
申请日：2002-09-03
IPC分类号： C12N500
CPC分类号： C12N5/0686 , A61K39/12 , C12N7/00 , C12N2500/92 , C12N2510/02 , C12N2710/16634 , C12N2760/16134 , C12N2760/16234 , C12N2760/18434 , C12N2760/18734 , C12N2770/36234
摘要： A novel MDCK-derived cell line (B-702) is provided that can be grown in the absence of fetal calf serum and in suspension culture for allowing a tank culture on the production scale and needs no carrier in suspension culture, as well as a process for producing virus for vaccine by using said cell line in a low-cost, highly safe and stable manner.
摘要翻译： 提供了一种新型的MDCK衍生的细胞系（B-702），其可以在没有胎牛血清的情况下生长，并且在悬浮培养物中生长以允许罐培养在生产规模上，并且不需要载体悬浮培养，以及 通过以低成本，高度安全和稳定的方式使用所述细胞系来生产疫苗病毒的方法。
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公开(公告)号：US6114143A
公开(公告)日：2000-09-05
申请号：US513968
申请日：1995-09-11
申请人： Yasuyuki Eda , Hiroaki Maeda , Keiichi Makizumi , Kouichi Shiosaki , Kiyoshi Osatomi , Kazuhiko Kimachi , Hirofumi Higuchi , Sachio Tokiyoshi
发明人： Yasuyuki Eda , Hiroaki Maeda , Keiichi Makizumi , Kouichi Shiosaki , Kiyoshi Osatomi , Kazuhiko Kimachi , Hirofumi Higuchi , Sachio Tokiyoshi
CPC分类号： C07K16/1063 , A61K38/00 , C07K2317/24
摘要： A monoclonal antibody useful for clinical application which recognizes the conserved region of V3-PND region of glycoprotein antigen having a molecular weight of about 1.2.times.10.sup.5 daltons (gp120) on a coating membrane of human immunodeficiency virus (HIV) and which has an ability to neutralize a broad range of various HIV variants, or a fragment thereof, and the chimeric and humanized antibodies derived therefrom are provided. By using as an immunogen a plurality of peptides having PND-Tip region containing the highly conserved GPGR sequence within PND of HIV gp120, a monoclonal antibody having a neutralizing activity to many HIV variants can be prepared. By transplanting the gene fragment coding for the variable region of said monoclonal antibody or complementarity determining region (CDR) of said region to a human antibody gene, a chimeric antibody or a reshaped antibody having an anti-HIV neutralizing activity which are effective for clinical application can be obtained.
摘要翻译： PCT No.PCT / JP94 / 00371 Sec。 371 1995年9月11日第 102（e）1995年9月11日PCT PCT 1994年3月9日PCT公布。 公开号WO94 / 20632 日期1994年9月15日一种可用于临床应用的单克隆抗体，其识别在人免疫缺陷病毒（HIV）的包被膜上具有约1.2×10 5道尔顿（gp120）分子量的糖蛋白抗原的V3-PND区的保守区， 具有中和各种各样的HIV变体或其片段的能力，并且提供了由其衍生的嵌合和人源化抗体。 通过使用多种具有含有HIV gp120的PND内的高度保守的GPGR序列的PND-Tip区的肽，可以制备对许多HIV变体具有中和活性的单克隆抗体。 通过将编码所述区域的所述单克隆抗体或互补决定区（CDR）的可变区的基因片段移植到人抗体基因，嵌合抗体或具有抗HIV中和活性的重构抗体，其对临床应用有效 可以获得。
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公开(公告)号：US20060280751A1
公开(公告)日：2006-12-14
申请号：US10546293
申请日：2004-02-18
CPC分类号： C07K16/00 , A61K2039/505 , C07K16/1063 , C07K2317/34
摘要： A method for enhancing efficacy of a monoclonal antibody preparation is provided wherein antigens from patients are tested for their reactivity with said antibody. In accordance with the method of the invention, an amino acid sequence of an expressed protein is deduced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in biopsy from patients and is compared with the previously determined amino acid sequence recognized by said monoclonal antibody preparation to thereby assess fitness of patients for administration of said monoclonal antibody preparation.
摘要翻译： 提供了增强单克隆抗体制剂功效的方法，其中测试来自患者的抗原与其抗体的反应性。 根据本发明的方法，通过从患者的活组织检查中分离和分析靶分子基因而确定的核苷酸序列推导出表达蛋白质的氨基酸序列，并将其与先前确定的所述 从而评估患者适用于所述单克隆抗体制剂的施用。
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公开(公告)号：US08332159B2
公开(公告)日：2012-12-11
申请号：US10546293
申请日：2004-02-18
IPC分类号： G06F7/00
CPC分类号： C07K16/00 , A61K2039/505 , C07K16/1063 , C07K2317/34
摘要： A method of enhancing the efficacy of a monoclonal antibody preparation wherein antigens from patients are tested for their reactivity with the antibody. An amino acid sequence of an expressed protein is deuced from a nucleotide sequence determined by isolation and analysis of a target molecule gene in a biopsy from a patient. This is compared with the previously determined amino acid sequence recognized by the monoclonal antibody preparation in order to assess the fitness of patients for administration of the monoclonal antibody preparation.
摘要翻译： 一种提高单克隆抗体制剂的功效的方法，其中测试来自患者的抗原与抗体的反应性。 通过从患者的活组织检查中分离和分析靶分子基因而确定的核苷酸序列，从表达的蛋白质的氨基酸序列中去除。 将其与由单克隆抗体制剂识别的先前确定的氨基酸序列进行比较，以评估患者施用单克隆抗体制剂的适合性。
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