Optical sensor for in situ measurement of analytes
    4.
    再颁专利
    Optical sensor for in situ measurement of analytes 有权
    用于原位测量分析物的光学传感器

    公开(公告)号:USRE38525E1

    公开(公告)日:2004-06-08

    申请号:US10277944

    申请日:2002-10-23

    IPC分类号: A61B5145

    CPC分类号: G01N33/54366 G01N33/54373

    摘要: An implantable sensor for use in the detection of quantitative measurement of an analyte in subcutaneous fluid, the sensor being biodegradable or hydrolysable in vivo. The sensor incorporates an assay for the analyte, the readout of which is a detectable or measurable optical signal which can, when the sensor is in operation in a subcutaneous location, be interrogated transcutaneously by external optical means.

    Capture of micro-organisms
    6.
    发明授权
    Capture of micro-organisms 有权
    捕获微生物

    公开(公告)号:US08603771B2

    公开(公告)日:2013-12-10

    申请号:US13813637

    申请日:2011-06-29

    IPC分类号: C12Q1/04 C12Q1/24

    摘要: Micro-organisms, including fungi, viruses and bacteria such as Mycobacteria and/or fragments of micro-organisms such as cell wall components present in an aqueous liquid are captured to a solid surface by adding to the liquid a sufficient quantity of a water soluble polymer in the presence of the solid surface to displace the micro-organisms and/or fragments from the liquid to the solid surface. The surface may be provided by a bead. The water soluble polymer may be polyethyleneglycol or polyvinylpyrrolidone.

    摘要翻译: 微生物包括真菌,病毒和细菌如分枝杆菌和/或微生物碎片如存在于水性液体中的细胞壁组分被捕获到固体表面,通过向液体中加入足量的水溶性聚合物 在固体表面的存在下将微生物和/或碎片从液体移位到固体表面。 表面可以由珠提供。 水溶性聚合物可以是聚乙二醇或聚乙烯吡咯烷酮。

    Use of nucleic acid analogues in the inhibition of nucleic acid
amplification
    10.
    发明授权
    Use of nucleic acid analogues in the inhibition of nucleic acid amplification 失效
    核酸类似物在抑制核酸扩增中的应用

    公开(公告)号:US5891625A

    公开(公告)日:1999-04-06

    申请号:US338535

    申请日:1995-03-10

    CPC分类号: C12Q1/6848 C12Q1/686

    摘要: Nucleic acid analogues such as peptide-nucleic acids (PNAs) which hybridize strongly to nucleic acids are used to inhibit nucleic acid amplification procedures such as PCR. False positives in subsequent PCR assays are prevented by hybridizing a PNA to PCR amplification products. Assays capable of discriminating between single base mutants are conducted by using a PNA hybridizing to one of the two allelic forms to inhibit a PCR amplification of that form selectively. Asymmetric PCR amplifications are carried out by starting a PCR symmetrically using like quantities of forward and reverse primers, and, once the amplification is established, disabling one primer by hybridizing a PNA thereto.

    摘要翻译: PCT No.PCT / EP93 / 01435 Sec。 371 1995年3月10日 102(e)1995年3月10日PCT PCT 1993年6月7日PCT公布。 公开号WO93 / 25706 日期1993年12月23日使用与核酸强烈杂交的核酸类似物如肽 - 核酸(PNA)来抑制核酸扩增程序如PCR。 通过将PNA与PCR扩增产物杂交来防止后续PCR测定中的假阳性。 能够区分单碱基突变体的测定通过使用与两种等位基因形式中的一种杂交的PNA进行,以选择性地抑制该形式的PCR扩增。 通过使用类似量的正向引物和反向引物对称地进行PCR来进行不对称PCR扩增,一旦扩增成功,通过与PNA杂交就能使一个引物失活。